Limits...
Novel poxvirus in big brown bats, northwestern United States.

Emerson GL, Nordhausen R, Garner MM, Huckabee JR, Johnson S, Wohrle RD, Davidson WB, Wilkins K, Li Y, Doty JB, Gallardo-Romero NF, Metcalfe MG, Karem KL, Damon IK, Carroll DS - Emerging Infect. Dis. (2013)

Bottom Line: Wing and joint tissues were positive by PCR for poxvirus.Thin-section electron microscopy showed poxvirus particles within A-type inclusions.Phylogenetic comparison supports establishment of a new genus of Poxviridae.

View Article: PubMed Central - PubMed

Affiliation: Poxvirus Program, Centers for Disease Control and Prevention, 1600 Clifton Rd NE, Mailstop G06, Atlanta, GA 30333, USA. gemerson@cdc.gov

ABSTRACT
A wildlife hospital and rehabilitation center in northwestern United States received several big brown bats with necrosuppurative osteomyelitis in multiple joints. Wing and joint tissues were positive by PCR for poxvirus. Thin-section electron microscopy showed poxvirus particles within A-type inclusions. Phylogenetic comparison supports establishment of a new genus of Poxviridae.

Show MeSH

Related in: MedlinePlus

Maximum clade credibility tree generated by MrBayes in Geneious version 6.0.5 (www.geneious.com/) using amino acid sequences from 7 open reading frames (final chain length 240,000 at an average SD of split frequencies of zero) for poxviruses. Clade credibility values are indicated at each node.The virus isolated in this study is shown in boldface. Amsacta moorei (red hairy caterpiller) entomopoxvirus (Moyer) was used as the outgroup. Scale bar indicates amino acid substitutions per site.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3713833&req=5

Figure 2: Maximum clade credibility tree generated by MrBayes in Geneious version 6.0.5 (www.geneious.com/) using amino acid sequences from 7 open reading frames (final chain length 240,000 at an average SD of split frequencies of zero) for poxviruses. Clade credibility values are indicated at each node.The virus isolated in this study is shown in boldface. Amsacta moorei (red hairy caterpiller) entomopoxvirus (Moyer) was used as the outgroup. Scale bar indicates amino acid substitutions per site.

Mentions: Genome sequencing produced data that were used to construct a phylogenetic tree (Figure 2). Virus DNA sequence data were collected by using the Illumina platform (www.illumina.com/technology/sequencing_technology.ilmn). DNA sequences from 7 open reading frames (A7L, A10L, A24R, D1R, D5R, H4L, and J6R, according to reference sequence vaccinia virus Copenhagen) were extracted on the basis of sequence similarity. Data were deposited in GenBank under accession nos. KC181855–KC181861. Open reading frames were translated into amino acid sequences and aligned by using the ClustalW alignment option in Geneious version 6.0.5 (www.geneious.com/). The tree search was conducted by using MrBayes in Geneious 6.0.5 under default settings and Amsacta moorei (red hairy caterpillar) entomopoxvirus (Moyer) was used as the outgroup with a burn in of 10%.


Novel poxvirus in big brown bats, northwestern United States.

Emerson GL, Nordhausen R, Garner MM, Huckabee JR, Johnson S, Wohrle RD, Davidson WB, Wilkins K, Li Y, Doty JB, Gallardo-Romero NF, Metcalfe MG, Karem KL, Damon IK, Carroll DS - Emerging Infect. Dis. (2013)

Maximum clade credibility tree generated by MrBayes in Geneious version 6.0.5 (www.geneious.com/) using amino acid sequences from 7 open reading frames (final chain length 240,000 at an average SD of split frequencies of zero) for poxviruses. Clade credibility values are indicated at each node.The virus isolated in this study is shown in boldface. Amsacta moorei (red hairy caterpiller) entomopoxvirus (Moyer) was used as the outgroup. Scale bar indicates amino acid substitutions per site.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3713833&req=5

Figure 2: Maximum clade credibility tree generated by MrBayes in Geneious version 6.0.5 (www.geneious.com/) using amino acid sequences from 7 open reading frames (final chain length 240,000 at an average SD of split frequencies of zero) for poxviruses. Clade credibility values are indicated at each node.The virus isolated in this study is shown in boldface. Amsacta moorei (red hairy caterpiller) entomopoxvirus (Moyer) was used as the outgroup. Scale bar indicates amino acid substitutions per site.
Mentions: Genome sequencing produced data that were used to construct a phylogenetic tree (Figure 2). Virus DNA sequence data were collected by using the Illumina platform (www.illumina.com/technology/sequencing_technology.ilmn). DNA sequences from 7 open reading frames (A7L, A10L, A24R, D1R, D5R, H4L, and J6R, according to reference sequence vaccinia virus Copenhagen) were extracted on the basis of sequence similarity. Data were deposited in GenBank under accession nos. KC181855–KC181861. Open reading frames were translated into amino acid sequences and aligned by using the ClustalW alignment option in Geneious version 6.0.5 (www.geneious.com/). The tree search was conducted by using MrBayes in Geneious 6.0.5 under default settings and Amsacta moorei (red hairy caterpillar) entomopoxvirus (Moyer) was used as the outgroup with a burn in of 10%.

Bottom Line: Wing and joint tissues were positive by PCR for poxvirus.Thin-section electron microscopy showed poxvirus particles within A-type inclusions.Phylogenetic comparison supports establishment of a new genus of Poxviridae.

View Article: PubMed Central - PubMed

Affiliation: Poxvirus Program, Centers for Disease Control and Prevention, 1600 Clifton Rd NE, Mailstop G06, Atlanta, GA 30333, USA. gemerson@cdc.gov

ABSTRACT
A wildlife hospital and rehabilitation center in northwestern United States received several big brown bats with necrosuppurative osteomyelitis in multiple joints. Wing and joint tissues were positive by PCR for poxvirus. Thin-section electron microscopy showed poxvirus particles within A-type inclusions. Phylogenetic comparison supports establishment of a new genus of Poxviridae.

Show MeSH
Related in: MedlinePlus