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Novel poxvirus in big brown bats, northwestern United States.

Emerson GL, Nordhausen R, Garner MM, Huckabee JR, Johnson S, Wohrle RD, Davidson WB, Wilkins K, Li Y, Doty JB, Gallardo-Romero NF, Metcalfe MG, Karem KL, Damon IK, Carroll DS - Emerging Infect. Dis. (2013)

Bottom Line: Wing and joint tissues were positive by PCR for poxvirus.Thin-section electron microscopy showed poxvirus particles within A-type inclusions.Phylogenetic comparison supports establishment of a new genus of Poxviridae.

View Article: PubMed Central - PubMed

Affiliation: Poxvirus Program, Centers for Disease Control and Prevention, 1600 Clifton Rd NE, Mailstop G06, Atlanta, GA 30333, USA. gemerson@cdc.gov

ABSTRACT
A wildlife hospital and rehabilitation center in northwestern United States received several big brown bats with necrosuppurative osteomyelitis in multiple joints. Wing and joint tissues were positive by PCR for poxvirus. Thin-section electron microscopy showed poxvirus particles within A-type inclusions. Phylogenetic comparison supports establishment of a new genus of Poxviridae.

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A) Electron micrograph of poxvirus particles in synovium of a big brown bat, northwestern United States. B) Negative staining of poxvirus particles in cell culture supernatant. Scale bar = 100 nm.
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Figure 1: A) Electron micrograph of poxvirus particles in synovium of a big brown bat, northwestern United States. B) Negative staining of poxvirus particles in cell culture supernatant. Scale bar = 100 nm.

Mentions: Thin-section electron microscopy of synovial tissue extracted from a wax histoblock showed poxvirus particles in inflammatory cells (Figure 1, panel A). A 906E transmission electron microscope (Carl Zeiss, Peabody, MA, USA) at an accelerating voltage of 80 kV was used for initial imaging. Digital images were captured by using a 2K × 2K camera (Advanced Microscopy Techniques, Danvers, MA, USA). The state veterinarian and the Centers for Disease Control and Prevention (Atlanta, GA, USA) were subsequently consulted.


Novel poxvirus in big brown bats, northwestern United States.

Emerson GL, Nordhausen R, Garner MM, Huckabee JR, Johnson S, Wohrle RD, Davidson WB, Wilkins K, Li Y, Doty JB, Gallardo-Romero NF, Metcalfe MG, Karem KL, Damon IK, Carroll DS - Emerging Infect. Dis. (2013)

A) Electron micrograph of poxvirus particles in synovium of a big brown bat, northwestern United States. B) Negative staining of poxvirus particles in cell culture supernatant. Scale bar = 100 nm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3713833&req=5

Figure 1: A) Electron micrograph of poxvirus particles in synovium of a big brown bat, northwestern United States. B) Negative staining of poxvirus particles in cell culture supernatant. Scale bar = 100 nm.
Mentions: Thin-section electron microscopy of synovial tissue extracted from a wax histoblock showed poxvirus particles in inflammatory cells (Figure 1, panel A). A 906E transmission electron microscope (Carl Zeiss, Peabody, MA, USA) at an accelerating voltage of 80 kV was used for initial imaging. Digital images were captured by using a 2K × 2K camera (Advanced Microscopy Techniques, Danvers, MA, USA). The state veterinarian and the Centers for Disease Control and Prevention (Atlanta, GA, USA) were subsequently consulted.

Bottom Line: Wing and joint tissues were positive by PCR for poxvirus.Thin-section electron microscopy showed poxvirus particles within A-type inclusions.Phylogenetic comparison supports establishment of a new genus of Poxviridae.

View Article: PubMed Central - PubMed

Affiliation: Poxvirus Program, Centers for Disease Control and Prevention, 1600 Clifton Rd NE, Mailstop G06, Atlanta, GA 30333, USA. gemerson@cdc.gov

ABSTRACT
A wildlife hospital and rehabilitation center in northwestern United States received several big brown bats with necrosuppurative osteomyelitis in multiple joints. Wing and joint tissues were positive by PCR for poxvirus. Thin-section electron microscopy showed poxvirus particles within A-type inclusions. Phylogenetic comparison supports establishment of a new genus of Poxviridae.

Show MeSH
Related in: MedlinePlus