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The effect of MTAD, an endodontic irrigant, on fibroblast attachment to periodontally affected root surfaces: A SEM analysis.

Ghandi M, Houshmand B, Nekoofar MH, Tabor RK, Yadeghari Z, Dummer PM - J Indian Soc Periodontol (2013)

Bottom Line: After fixation, the samples were sputter-coated with gold and examined with a SEM.The data was analysed using the Mann-Whitney-U statistical test.There was no significant difference between the numbers of attached cells in the experimental group treated with MTAD and the control group treated with saline.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontics, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

ABSTRACT

Background: Root surface debridement (RSD) is necessary to create an environment suitable for reattachment of the periodontium. Root surface conditioning may aid the formation of a biocompatible surface suitable for cell reattachment. BioPure™ MTAD (mixture of Doxycycline, citric acid and a detergent) is an endodontic irrigant with antibacterial properties and the ability to remove smear layer. It was hypothesized that MTAD may be useful for root surface conditioning. The efficacy of MTAD as a conditioner was measured by examining fibroblast attachment to root surfaces.

Materials and methods: Thirty-two specimens of human teeth with advanced periodontal disease were used. The surfaces were root planed until smooth. Half of the specimens were treated with 0.9% saline and the other samples with Biopure MTAD. As a negative control group, five further samples were left unscaled with surface calculus. Human gingival fibroblast cells HGF1-PI1 were cultured and poured over the tooth specimens and incubated. After fixation, the samples were sputter-coated with gold and examined with a SEM. The morphology and number of attached, fixed viable cells were examined. The data was analysed using the Mann-Whitney-U statistical test.

Results: There was no significant difference between the numbers of attached cells in the experimental group treated with MTAD and the control group treated with saline. Little or no attached cells were seen in the negative control group.

Conclusion: RSD created an environment suitable for cell growth and attachment in a laboratory setting. The use of MTAD did not promote the attachment and growth of cells on the surface of human roots following RSD.

No MeSH data available.


Related in: MedlinePlus

Root surface treated with saline after root surface debridement. (a) (original magnification ×350.) An example of one of the regions randomly selected by use of the template. (b) Selected region from (a) (original magnification ×1000). Viable, flat, well-attached cells with elongated processes attached to root surface covered by a heavy smear layer can be seen
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Figure 2: Root surface treated with saline after root surface debridement. (a) (original magnification ×350.) An example of one of the regions randomly selected by use of the template. (b) Selected region from (a) (original magnification ×1000). Viable, flat, well-attached cells with elongated processes attached to root surface covered by a heavy smear layer can be seen

Mentions: The tooth samples were then placed in gluteraldehyde for 24 hrs and immersed in distilled water for five minutes. The specimens in the experimental group were then freeze-dried for 12 hrs using a Modulyo freeze-dryer (Edwards, Crawley, UK). The surfaces were then sputter-coated with gold using a Polaron Sputter Coater (Quorum Technologies, Newhaven, UK) and specimens were examined with an EBT1 (Electron Beam Technology) Scanning Electron Microscope (S.E.M. Tech Ltd, Woodbridge, UK). The SEM images of the working root surfaces at a low magnification (×4) were overlaid with a template with five specific marked points to prevent operator bias. Each point was magnified to ×350. The morphology of cells present was examined by two independent fully calibrated examiners blind to the code given to the specimens. The only cells counted by the examiners were attached, flat cells with elongated processes [Figures 2 and 3]. The cells with this specific appearance were considered to be viable before fixation. The control coverslip was also examined following similar preparation using an EBT1 (Electron Beam Technology) Scanning Electron Microscope (S.E.M. Tech Ltd, Woodbridge, UK).


The effect of MTAD, an endodontic irrigant, on fibroblast attachment to periodontally affected root surfaces: A SEM analysis.

Ghandi M, Houshmand B, Nekoofar MH, Tabor RK, Yadeghari Z, Dummer PM - J Indian Soc Periodontol (2013)

Root surface treated with saline after root surface debridement. (a) (original magnification ×350.) An example of one of the regions randomly selected by use of the template. (b) Selected region from (a) (original magnification ×1000). Viable, flat, well-attached cells with elongated processes attached to root surface covered by a heavy smear layer can be seen
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3713749&req=5

Figure 2: Root surface treated with saline after root surface debridement. (a) (original magnification ×350.) An example of one of the regions randomly selected by use of the template. (b) Selected region from (a) (original magnification ×1000). Viable, flat, well-attached cells with elongated processes attached to root surface covered by a heavy smear layer can be seen
Mentions: The tooth samples were then placed in gluteraldehyde for 24 hrs and immersed in distilled water for five minutes. The specimens in the experimental group were then freeze-dried for 12 hrs using a Modulyo freeze-dryer (Edwards, Crawley, UK). The surfaces were then sputter-coated with gold using a Polaron Sputter Coater (Quorum Technologies, Newhaven, UK) and specimens were examined with an EBT1 (Electron Beam Technology) Scanning Electron Microscope (S.E.M. Tech Ltd, Woodbridge, UK). The SEM images of the working root surfaces at a low magnification (×4) were overlaid with a template with five specific marked points to prevent operator bias. Each point was magnified to ×350. The morphology of cells present was examined by two independent fully calibrated examiners blind to the code given to the specimens. The only cells counted by the examiners were attached, flat cells with elongated processes [Figures 2 and 3]. The cells with this specific appearance were considered to be viable before fixation. The control coverslip was also examined following similar preparation using an EBT1 (Electron Beam Technology) Scanning Electron Microscope (S.E.M. Tech Ltd, Woodbridge, UK).

Bottom Line: After fixation, the samples were sputter-coated with gold and examined with a SEM.The data was analysed using the Mann-Whitney-U statistical test.There was no significant difference between the numbers of attached cells in the experimental group treated with MTAD and the control group treated with saline.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontics, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

ABSTRACT

Background: Root surface debridement (RSD) is necessary to create an environment suitable for reattachment of the periodontium. Root surface conditioning may aid the formation of a biocompatible surface suitable for cell reattachment. BioPure™ MTAD (mixture of Doxycycline, citric acid and a detergent) is an endodontic irrigant with antibacterial properties and the ability to remove smear layer. It was hypothesized that MTAD may be useful for root surface conditioning. The efficacy of MTAD as a conditioner was measured by examining fibroblast attachment to root surfaces.

Materials and methods: Thirty-two specimens of human teeth with advanced periodontal disease were used. The surfaces were root planed until smooth. Half of the specimens were treated with 0.9% saline and the other samples with Biopure MTAD. As a negative control group, five further samples were left unscaled with surface calculus. Human gingival fibroblast cells HGF1-PI1 were cultured and poured over the tooth specimens and incubated. After fixation, the samples were sputter-coated with gold and examined with a SEM. The morphology and number of attached, fixed viable cells were examined. The data was analysed using the Mann-Whitney-U statistical test.

Results: There was no significant difference between the numbers of attached cells in the experimental group treated with MTAD and the control group treated with saline. Little or no attached cells were seen in the negative control group.

Conclusion: RSD created an environment suitable for cell growth and attachment in a laboratory setting. The use of MTAD did not promote the attachment and growth of cells on the surface of human roots following RSD.

No MeSH data available.


Related in: MedlinePlus