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The effect of MTAD, an endodontic irrigant, on fibroblast attachment to periodontally affected root surfaces: A SEM analysis.

Ghandi M, Houshmand B, Nekoofar MH, Tabor RK, Yadeghari Z, Dummer PM - J Indian Soc Periodontol (2013)

Bottom Line: There was no significant difference between the numbers of attached cells in the experimental group treated with MTAD and the control group treated with saline.RSD created an environment suitable for cell growth and attachment in a laboratory setting.The use of MTAD did not promote the attachment and growth of cells on the surface of human roots following RSD.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontics, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

ABSTRACT

Background: Root surface debridement (RSD) is necessary to create an environment suitable for reattachment of the periodontium. Root surface conditioning may aid the formation of a biocompatible surface suitable for cell reattachment. BioPureā„¢ MTAD (mixture of Doxycycline, citric acid and a detergent) is an endodontic irrigant with antibacterial properties and the ability to remove smear layer. It was hypothesized that MTAD may be useful for root surface conditioning. The efficacy of MTAD as a conditioner was measured by examining fibroblast attachment to root surfaces.

Materials and methods: Thirty-two specimens of human teeth with advanced periodontal disease were used. The surfaces were root planed until smooth. Half of the specimens were treated with 0.9% saline and the other samples with Biopure MTAD. As a negative control group, five further samples were left unscaled with surface calculus. Human gingival fibroblast cells HGF1-PI1 were cultured and poured over the tooth specimens and incubated. After fixation, the samples were sputter-coated with gold and examined with a SEM. The morphology and number of attached, fixed viable cells were examined. The data was analysed using the Mann-Whitney-U statistical test.

Results: There was no significant difference between the numbers of attached cells in the experimental group treated with MTAD and the control group treated with saline. Little or no attached cells were seen in the negative control group.

Conclusion: RSD created an environment suitable for cell growth and attachment in a laboratory setting. The use of MTAD did not promote the attachment and growth of cells on the surface of human roots following RSD.

No MeSH data available.


Related in: MedlinePlus

The addition of the liquid component of MTAD, including citric acid, to powder to form active MTAD
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Figure 1: The addition of the liquid component of MTAD, including citric acid, to powder to form active MTAD

Mentions: Biopure MTAD (Dentsply Tulsa Dental, Tulsa, OK, USA) [Figure 1] is a material originally developed for use during endodontic treatment as a final irrigant to remove the smear layer from the root canal walls prior to root canal filling.[19] It is a mixture of Doxycycline (a tetracycline isomer), citric acid and Polysorbate 80 (a detergent).[20] The efficacy of MTAD as a conditioner of root surfaces in periodontal treatment has not been investigated before. This ex vivo study aims to evaluate the effect of MTAD after RSD on the adherence of cultured fibroblasts, compared with RSD with saline treatment.


The effect of MTAD, an endodontic irrigant, on fibroblast attachment to periodontally affected root surfaces: A SEM analysis.

Ghandi M, Houshmand B, Nekoofar MH, Tabor RK, Yadeghari Z, Dummer PM - J Indian Soc Periodontol (2013)

The addition of the liquid component of MTAD, including citric acid, to powder to form active MTAD
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3713749&req=5

Figure 1: The addition of the liquid component of MTAD, including citric acid, to powder to form active MTAD
Mentions: Biopure MTAD (Dentsply Tulsa Dental, Tulsa, OK, USA) [Figure 1] is a material originally developed for use during endodontic treatment as a final irrigant to remove the smear layer from the root canal walls prior to root canal filling.[19] It is a mixture of Doxycycline (a tetracycline isomer), citric acid and Polysorbate 80 (a detergent).[20] The efficacy of MTAD as a conditioner of root surfaces in periodontal treatment has not been investigated before. This ex vivo study aims to evaluate the effect of MTAD after RSD on the adherence of cultured fibroblasts, compared with RSD with saline treatment.

Bottom Line: There was no significant difference between the numbers of attached cells in the experimental group treated with MTAD and the control group treated with saline.RSD created an environment suitable for cell growth and attachment in a laboratory setting.The use of MTAD did not promote the attachment and growth of cells on the surface of human roots following RSD.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontics, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

ABSTRACT

Background: Root surface debridement (RSD) is necessary to create an environment suitable for reattachment of the periodontium. Root surface conditioning may aid the formation of a biocompatible surface suitable for cell reattachment. BioPureā„¢ MTAD (mixture of Doxycycline, citric acid and a detergent) is an endodontic irrigant with antibacterial properties and the ability to remove smear layer. It was hypothesized that MTAD may be useful for root surface conditioning. The efficacy of MTAD as a conditioner was measured by examining fibroblast attachment to root surfaces.

Materials and methods: Thirty-two specimens of human teeth with advanced periodontal disease were used. The surfaces were root planed until smooth. Half of the specimens were treated with 0.9% saline and the other samples with Biopure MTAD. As a negative control group, five further samples were left unscaled with surface calculus. Human gingival fibroblast cells HGF1-PI1 were cultured and poured over the tooth specimens and incubated. After fixation, the samples were sputter-coated with gold and examined with a SEM. The morphology and number of attached, fixed viable cells were examined. The data was analysed using the Mann-Whitney-U statistical test.

Results: There was no significant difference between the numbers of attached cells in the experimental group treated with MTAD and the control group treated with saline. Little or no attached cells were seen in the negative control group.

Conclusion: RSD created an environment suitable for cell growth and attachment in a laboratory setting. The use of MTAD did not promote the attachment and growth of cells on the surface of human roots following RSD.

No MeSH data available.


Related in: MedlinePlus