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Evaluation of Nonradiative Clinical Imaging Techniques for the Longitudinal Assessment of Tumour Growth in Murine CT26 Colon Carcinoma.

Seguin J, Doan BT, Latorre Ossa H, Jugé L, Gennisson JL, Tanter M, Scherman D, Chabot GG, Mignet N - Int J Mol Imaging (2013)

Bottom Line: Methods.Monitoring of tumour growth with US could be accomplished as early as 5 days after implantation with a shorter acquisition time (<5 min) compared to MRI.Conclusion.

View Article: PubMed Central - PubMed

Affiliation: Chemical, Genetic and Imaging Pharmacology Laboratory, Faculty of Pharmacy, Chimie ParisTech, Paris Descartes University, Sorbonne Paris Cité, INSERM U1022, CNRS UMR8151, 4 Avenue de l'Observatoire, 75006 Paris, France.

ABSTRACT
Background and Objectives. To determine the most appropriate technique for tumour followup in experimental therapeutics, we compared ultrasound (US) and magnetic resonance imaging (MRI) to characterize ectopic and orthotopic colon carcinoma models. Methods. CT26 tumours were implanted subcutaneously (s.c.) in Balb/c mice for the ectopic model or into the caecum for the orthotopic model. Tumours were evaluated by histology, spectrofluorescence, MRI, and US. Results. Histology of CT26 tumour showed homogeneously dispersed cancer cells and blood vessels. The visualization of the vascular network using labelled albumin showed that CT26 tumours were highly vascularized and disorganized. MRI allowed high-resolution and accurate 3D tumour measurements and provided additional anatomical and functional information. Noninvasive US imaging allowed good delineation of tumours despite an hypoechogenic signal. Monitoring of tumour growth with US could be accomplished as early as 5 days after implantation with a shorter acquisition time (<5 min) compared to MRI. Conclusion. MRI and US afforded excellent noninvasive imaging techniques to accurately follow tumour growth of ectopic and orthotopic CT26 tumours. These two techniques can be appropriately used for tumour treatment followup, with a preference for US imaging, due to its short acquisition time and simplicity of use.

No MeSH data available.


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T2* weighted images showing the ectopic and orthotopic CT26 tumour. T2* weighted imaging provides anatomical axial images of ectopic (a) and orthotopic model (b) at days 5, 11, and 15. White arrows indicate the tumour localisation. Black arrows show the hyposignal regions. (c) Evaluation of tumour volume with images quantification for ectopic and orthotopic model as a function of time.
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fig4: T2* weighted images showing the ectopic and orthotopic CT26 tumour. T2* weighted imaging provides anatomical axial images of ectopic (a) and orthotopic model (b) at days 5, 11, and 15. White arrows indicate the tumour localisation. Black arrows show the hyposignal regions. (c) Evaluation of tumour volume with images quantification for ectopic and orthotopic model as a function of time.

Mentions: We next explored the characterization of colon carcinoma CT26 models using MRI. Coronal image was obtained from T2*-weighted sequences in which axial slices were positioned in the tumour area (see Figure 1 in the supplementary material available online at http://dx.doi.org/10.1155/2013/983534). In these axial slices, tumour burden was easily detected without the use of a contrast agent. The tumour mass appeared in grey and the outline was relatively well defined as shown in Figures 4(a) and 4(b) (white arrows). From these images, it was possible to calculate the tumour volume by the addition of the surface areas of the consecutive axial slices taken in the tumour mass. Small tumours, ranging from 15 to 20 mm3 measured at day 5, to large tumours up to 3000–3400 mm3 measured at day 18, are shown on Figure 4(c). Tumour growth obtained by MRI followed a similar profile for both models with a significant difference on days 11 and 15.


Evaluation of Nonradiative Clinical Imaging Techniques for the Longitudinal Assessment of Tumour Growth in Murine CT26 Colon Carcinoma.

Seguin J, Doan BT, Latorre Ossa H, Jugé L, Gennisson JL, Tanter M, Scherman D, Chabot GG, Mignet N - Int J Mol Imaging (2013)

T2* weighted images showing the ectopic and orthotopic CT26 tumour. T2* weighted imaging provides anatomical axial images of ectopic (a) and orthotopic model (b) at days 5, 11, and 15. White arrows indicate the tumour localisation. Black arrows show the hyposignal regions. (c) Evaluation of tumour volume with images quantification for ectopic and orthotopic model as a function of time.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3713650&req=5

fig4: T2* weighted images showing the ectopic and orthotopic CT26 tumour. T2* weighted imaging provides anatomical axial images of ectopic (a) and orthotopic model (b) at days 5, 11, and 15. White arrows indicate the tumour localisation. Black arrows show the hyposignal regions. (c) Evaluation of tumour volume with images quantification for ectopic and orthotopic model as a function of time.
Mentions: We next explored the characterization of colon carcinoma CT26 models using MRI. Coronal image was obtained from T2*-weighted sequences in which axial slices were positioned in the tumour area (see Figure 1 in the supplementary material available online at http://dx.doi.org/10.1155/2013/983534). In these axial slices, tumour burden was easily detected without the use of a contrast agent. The tumour mass appeared in grey and the outline was relatively well defined as shown in Figures 4(a) and 4(b) (white arrows). From these images, it was possible to calculate the tumour volume by the addition of the surface areas of the consecutive axial slices taken in the tumour mass. Small tumours, ranging from 15 to 20 mm3 measured at day 5, to large tumours up to 3000–3400 mm3 measured at day 18, are shown on Figure 4(c). Tumour growth obtained by MRI followed a similar profile for both models with a significant difference on days 11 and 15.

Bottom Line: Methods.Monitoring of tumour growth with US could be accomplished as early as 5 days after implantation with a shorter acquisition time (<5 min) compared to MRI.Conclusion.

View Article: PubMed Central - PubMed

Affiliation: Chemical, Genetic and Imaging Pharmacology Laboratory, Faculty of Pharmacy, Chimie ParisTech, Paris Descartes University, Sorbonne Paris Cité, INSERM U1022, CNRS UMR8151, 4 Avenue de l'Observatoire, 75006 Paris, France.

ABSTRACT
Background and Objectives. To determine the most appropriate technique for tumour followup in experimental therapeutics, we compared ultrasound (US) and magnetic resonance imaging (MRI) to characterize ectopic and orthotopic colon carcinoma models. Methods. CT26 tumours were implanted subcutaneously (s.c.) in Balb/c mice for the ectopic model or into the caecum for the orthotopic model. Tumours were evaluated by histology, spectrofluorescence, MRI, and US. Results. Histology of CT26 tumour showed homogeneously dispersed cancer cells and blood vessels. The visualization of the vascular network using labelled albumin showed that CT26 tumours were highly vascularized and disorganized. MRI allowed high-resolution and accurate 3D tumour measurements and provided additional anatomical and functional information. Noninvasive US imaging allowed good delineation of tumours despite an hypoechogenic signal. Monitoring of tumour growth with US could be accomplished as early as 5 days after implantation with a shorter acquisition time (<5 min) compared to MRI. Conclusion. MRI and US afforded excellent noninvasive imaging techniques to accurately follow tumour growth of ectopic and orthotopic CT26 tumours. These two techniques can be appropriately used for tumour treatment followup, with a preference for US imaging, due to its short acquisition time and simplicity of use.

No MeSH data available.


Related in: MedlinePlus