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Evaluation of Nonradiative Clinical Imaging Techniques for the Longitudinal Assessment of Tumour Growth in Murine CT26 Colon Carcinoma.

Seguin J, Doan BT, Latorre Ossa H, Jugé L, Gennisson JL, Tanter M, Scherman D, Chabot GG, Mignet N - Int J Mol Imaging (2013)

Bottom Line: Methods.Monitoring of tumour growth with US could be accomplished as early as 5 days after implantation with a shorter acquisition time (<5 min) compared to MRI.Conclusion.

View Article: PubMed Central - PubMed

Affiliation: Chemical, Genetic and Imaging Pharmacology Laboratory, Faculty of Pharmacy, Chimie ParisTech, Paris Descartes University, Sorbonne Paris Cité, INSERM U1022, CNRS UMR8151, 4 Avenue de l'Observatoire, 75006 Paris, France.

ABSTRACT
Background and Objectives. To determine the most appropriate technique for tumour followup in experimental therapeutics, we compared ultrasound (US) and magnetic resonance imaging (MRI) to characterize ectopic and orthotopic colon carcinoma models. Methods. CT26 tumours were implanted subcutaneously (s.c.) in Balb/c mice for the ectopic model or into the caecum for the orthotopic model. Tumours were evaluated by histology, spectrofluorescence, MRI, and US. Results. Histology of CT26 tumour showed homogeneously dispersed cancer cells and blood vessels. The visualization of the vascular network using labelled albumin showed that CT26 tumours were highly vascularized and disorganized. MRI allowed high-resolution and accurate 3D tumour measurements and provided additional anatomical and functional information. Noninvasive US imaging allowed good delineation of tumours despite an hypoechogenic signal. Monitoring of tumour growth with US could be accomplished as early as 5 days after implantation with a shorter acquisition time (<5 min) compared to MRI. Conclusion. MRI and US afforded excellent noninvasive imaging techniques to accurately follow tumour growth of ectopic and orthotopic CT26 tumours. These two techniques can be appropriately used for tumour treatment followup, with a preference for US imaging, due to its short acquisition time and simplicity of use.

No MeSH data available.


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Histology of CT26 tumours implanted into the intestinal mucosa. Histological images were obtained using haematoxylin counterstain for healthy caecum without tumour (a), ectopically implanted CT26 tumours (b), and orthotopic CT26 tumour (c). Tumours were harvested 21 days after tumour implantation. Original magnification 100x, scale bar 200 μm). Lu, lumen; Ml, muscularis; Mu, mucosa; Se, serosa; T, tumour.
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fig2: Histology of CT26 tumours implanted into the intestinal mucosa. Histological images were obtained using haematoxylin counterstain for healthy caecum without tumour (a), ectopically implanted CT26 tumours (b), and orthotopic CT26 tumour (c). Tumours were harvested 21 days after tumour implantation. Original magnification 100x, scale bar 200 μm). Lu, lumen; Ml, muscularis; Mu, mucosa; Se, serosa; T, tumour.

Mentions: We next verified the correct implantation of the orthotopic tumour into the intestinal mucosa. Figure 2 presents a healthy caecum without tumour (Figure 2(a)) and an ectopically implanted CT26 tumour (Figure 2(b)) where the tumour cells can be seen. In Figure 2(c), the histological examination of orthotopic slices clearly shows the invasion of the caecal serosa and the muscularis by the tumour cells.


Evaluation of Nonradiative Clinical Imaging Techniques for the Longitudinal Assessment of Tumour Growth in Murine CT26 Colon Carcinoma.

Seguin J, Doan BT, Latorre Ossa H, Jugé L, Gennisson JL, Tanter M, Scherman D, Chabot GG, Mignet N - Int J Mol Imaging (2013)

Histology of CT26 tumours implanted into the intestinal mucosa. Histological images were obtained using haematoxylin counterstain for healthy caecum without tumour (a), ectopically implanted CT26 tumours (b), and orthotopic CT26 tumour (c). Tumours were harvested 21 days after tumour implantation. Original magnification 100x, scale bar 200 μm). Lu, lumen; Ml, muscularis; Mu, mucosa; Se, serosa; T, tumour.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3713650&req=5

fig2: Histology of CT26 tumours implanted into the intestinal mucosa. Histological images were obtained using haematoxylin counterstain for healthy caecum without tumour (a), ectopically implanted CT26 tumours (b), and orthotopic CT26 tumour (c). Tumours were harvested 21 days after tumour implantation. Original magnification 100x, scale bar 200 μm). Lu, lumen; Ml, muscularis; Mu, mucosa; Se, serosa; T, tumour.
Mentions: We next verified the correct implantation of the orthotopic tumour into the intestinal mucosa. Figure 2 presents a healthy caecum without tumour (Figure 2(a)) and an ectopically implanted CT26 tumour (Figure 2(b)) where the tumour cells can be seen. In Figure 2(c), the histological examination of orthotopic slices clearly shows the invasion of the caecal serosa and the muscularis by the tumour cells.

Bottom Line: Methods.Monitoring of tumour growth with US could be accomplished as early as 5 days after implantation with a shorter acquisition time (<5 min) compared to MRI.Conclusion.

View Article: PubMed Central - PubMed

Affiliation: Chemical, Genetic and Imaging Pharmacology Laboratory, Faculty of Pharmacy, Chimie ParisTech, Paris Descartes University, Sorbonne Paris Cité, INSERM U1022, CNRS UMR8151, 4 Avenue de l'Observatoire, 75006 Paris, France.

ABSTRACT
Background and Objectives. To determine the most appropriate technique for tumour followup in experimental therapeutics, we compared ultrasound (US) and magnetic resonance imaging (MRI) to characterize ectopic and orthotopic colon carcinoma models. Methods. CT26 tumours were implanted subcutaneously (s.c.) in Balb/c mice for the ectopic model or into the caecum for the orthotopic model. Tumours were evaluated by histology, spectrofluorescence, MRI, and US. Results. Histology of CT26 tumour showed homogeneously dispersed cancer cells and blood vessels. The visualization of the vascular network using labelled albumin showed that CT26 tumours were highly vascularized and disorganized. MRI allowed high-resolution and accurate 3D tumour measurements and provided additional anatomical and functional information. Noninvasive US imaging allowed good delineation of tumours despite an hypoechogenic signal. Monitoring of tumour growth with US could be accomplished as early as 5 days after implantation with a shorter acquisition time (<5 min) compared to MRI. Conclusion. MRI and US afforded excellent noninvasive imaging techniques to accurately follow tumour growth of ectopic and orthotopic CT26 tumours. These two techniques can be appropriately used for tumour treatment followup, with a preference for US imaging, due to its short acquisition time and simplicity of use.

No MeSH data available.


Related in: MedlinePlus