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A mouse model of interstitial pneumonitis induced by murine cytomegalovirus infection after allogeneic skin transplantation.

Ni D, Yu H, Zhang W, Gan L, Zhao J, Wang M, Chen J - Biomed Res Int (2013)

Bottom Line: Flow cytometry showed that the number of CD4(+) and CD8(+) cells and the level of IFN- γ decreased significantly in the groups treated with cyclosporin A.Transmission electronic microscopy demonstrated the presence of herpes virus particles.MCMV DNA and glycoprotein B were demonstrated in the epithelial cells of the lung tissue in those animals by in situ hybridization and immunohistochemistry, respectively.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Anhui Medical University, Hefei, Anhui 230032, China.

ABSTRACT
We investigated the effect of murine cytomegalovirus (MCMV) on interstitial pneumonia in transplant recipients in an experimental skin allograft model. Skin transplantation between C57BL/6J and BALB/c mice was performed in the presence or absence of cyclosporin A treatment. Flow cytometry showed that the number of CD4(+) and CD8(+) cells and the level of IFN- γ decreased significantly in the groups treated with cyclosporin A. We either mock-infected or infected the mice with MCMV by intranasal administration and monitored pathophysiological behavior and body weight. The infected mice were sacrificed at different days postinfection for histology, immunohistochemistry, and molecular biological evaluations. Interstitial pneumonitis was observed in positive control groups as well as in experimental group that received cyclosporin A, a skin transplant, and infected with the highest dose of virus (10(5) PFU). Transmission electronic microscopy demonstrated the presence of herpes virus particles. MCMV DNA and glycoprotein B were demonstrated in the epithelial cells of the lung tissue in those animals by in situ hybridization and immunohistochemistry, respectively. Our data demonstrated the establishment of a mouse model of interstitial pneumonitis via MCMV infection after allogeneic skin transplantation.

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Herpes-like virus particles in the alveoli of lung tissues as observed by TEM. (a) Herpes virus-like nucleocapsids (arrows) were present in an epithelial cell of the alveoli of a mouse of Group C 21 days postinfection (×50,000). (b) An inclusion body was found in the cytoplasm of an epithelial cell of the alveoli of a mouse in Group D 21 days postinfection (×30,000).
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fig6: Herpes-like virus particles in the alveoli of lung tissues as observed by TEM. (a) Herpes virus-like nucleocapsids (arrows) were present in an epithelial cell of the alveoli of a mouse of Group C 21 days postinfection (×50,000). (b) An inclusion body was found in the cytoplasm of an epithelial cell of the alveoli of a mouse in Group D 21 days postinfection (×30,000).

Mentions: TEM was used to visualize the viral particles of MCMV in the lung tissue of infected mice. Herpes-like virus particles and inclusion bodies were imaged by TEM in the epithelium of the lung tissue in mice of Control AV, Control BV, Control CV, Group C, and Group D (Figure 6). None of the virus particles or inclusion bodies were found by TEM in the lung tissue of other mice groups.


A mouse model of interstitial pneumonitis induced by murine cytomegalovirus infection after allogeneic skin transplantation.

Ni D, Yu H, Zhang W, Gan L, Zhao J, Wang M, Chen J - Biomed Res Int (2013)

Herpes-like virus particles in the alveoli of lung tissues as observed by TEM. (a) Herpes virus-like nucleocapsids (arrows) were present in an epithelial cell of the alveoli of a mouse of Group C 21 days postinfection (×50,000). (b) An inclusion body was found in the cytoplasm of an epithelial cell of the alveoli of a mouse in Group D 21 days postinfection (×30,000).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3713605&req=5

fig6: Herpes-like virus particles in the alveoli of lung tissues as observed by TEM. (a) Herpes virus-like nucleocapsids (arrows) were present in an epithelial cell of the alveoli of a mouse of Group C 21 days postinfection (×50,000). (b) An inclusion body was found in the cytoplasm of an epithelial cell of the alveoli of a mouse in Group D 21 days postinfection (×30,000).
Mentions: TEM was used to visualize the viral particles of MCMV in the lung tissue of infected mice. Herpes-like virus particles and inclusion bodies were imaged by TEM in the epithelium of the lung tissue in mice of Control AV, Control BV, Control CV, Group C, and Group D (Figure 6). None of the virus particles or inclusion bodies were found by TEM in the lung tissue of other mice groups.

Bottom Line: Flow cytometry showed that the number of CD4(+) and CD8(+) cells and the level of IFN- γ decreased significantly in the groups treated with cyclosporin A.Transmission electronic microscopy demonstrated the presence of herpes virus particles.MCMV DNA and glycoprotein B were demonstrated in the epithelial cells of the lung tissue in those animals by in situ hybridization and immunohistochemistry, respectively.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Anhui Medical University, Hefei, Anhui 230032, China.

ABSTRACT
We investigated the effect of murine cytomegalovirus (MCMV) on interstitial pneumonia in transplant recipients in an experimental skin allograft model. Skin transplantation between C57BL/6J and BALB/c mice was performed in the presence or absence of cyclosporin A treatment. Flow cytometry showed that the number of CD4(+) and CD8(+) cells and the level of IFN- γ decreased significantly in the groups treated with cyclosporin A. We either mock-infected or infected the mice with MCMV by intranasal administration and monitored pathophysiological behavior and body weight. The infected mice were sacrificed at different days postinfection for histology, immunohistochemistry, and molecular biological evaluations. Interstitial pneumonitis was observed in positive control groups as well as in experimental group that received cyclosporin A, a skin transplant, and infected with the highest dose of virus (10(5) PFU). Transmission electronic microscopy demonstrated the presence of herpes virus particles. MCMV DNA and glycoprotein B were demonstrated in the epithelial cells of the lung tissue in those animals by in situ hybridization and immunohistochemistry, respectively. Our data demonstrated the establishment of a mouse model of interstitial pneumonitis via MCMV infection after allogeneic skin transplantation.

Show MeSH
Related in: MedlinePlus