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A mouse model of interstitial pneumonitis induced by murine cytomegalovirus infection after allogeneic skin transplantation.

Ni D, Yu H, Zhang W, Gan L, Zhao J, Wang M, Chen J - Biomed Res Int (2013)

Bottom Line: Flow cytometry showed that the number of CD4(+) and CD8(+) cells and the level of IFN- γ decreased significantly in the groups treated with cyclosporin A.Transmission electronic microscopy demonstrated the presence of herpes virus particles.MCMV DNA and glycoprotein B were demonstrated in the epithelial cells of the lung tissue in those animals by in situ hybridization and immunohistochemistry, respectively.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Anhui Medical University, Hefei, Anhui 230032, China.

ABSTRACT
We investigated the effect of murine cytomegalovirus (MCMV) on interstitial pneumonia in transplant recipients in an experimental skin allograft model. Skin transplantation between C57BL/6J and BALB/c mice was performed in the presence or absence of cyclosporin A treatment. Flow cytometry showed that the number of CD4(+) and CD8(+) cells and the level of IFN- γ decreased significantly in the groups treated with cyclosporin A. We either mock-infected or infected the mice with MCMV by intranasal administration and monitored pathophysiological behavior and body weight. The infected mice were sacrificed at different days postinfection for histology, immunohistochemistry, and molecular biological evaluations. Interstitial pneumonitis was observed in positive control groups as well as in experimental group that received cyclosporin A, a skin transplant, and infected with the highest dose of virus (10(5) PFU). Transmission electronic microscopy demonstrated the presence of herpes virus particles. MCMV DNA and glycoprotein B were demonstrated in the epithelial cells of the lung tissue in those animals by in situ hybridization and immunohistochemistry, respectively. Our data demonstrated the establishment of a mouse model of interstitial pneumonitis via MCMV infection after allogeneic skin transplantation.

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(a) The body weight of mice of the control groups (Controls A, B, C, AV, BV, and CV) at different days postinfection. (b) The body weight of mice of the experimental groups (Groups A–E) at different times after MCMV infection.
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fig2: (a) The body weight of mice of the control groups (Controls A, B, C, AV, BV, and CV) at different days postinfection. (b) The body weight of mice of the experimental groups (Groups A–E) at different times after MCMV infection.

Mentions: Furthermore, the growth of MCMV-infected mice (Controls AV, BV, and CV), as measured by body weight, was slower than those of the corresponding control groups (Controls A, B, and C) (Figure 2(a)) (F = 9.213, P < 0.05). The growth of mice in group D was also slower than the growth of the uninfected mice of Group E (F = 10.492, P < 0.01) (Figure 2(b)).


A mouse model of interstitial pneumonitis induced by murine cytomegalovirus infection after allogeneic skin transplantation.

Ni D, Yu H, Zhang W, Gan L, Zhao J, Wang M, Chen J - Biomed Res Int (2013)

(a) The body weight of mice of the control groups (Controls A, B, C, AV, BV, and CV) at different days postinfection. (b) The body weight of mice of the experimental groups (Groups A–E) at different times after MCMV infection.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3713605&req=5

fig2: (a) The body weight of mice of the control groups (Controls A, B, C, AV, BV, and CV) at different days postinfection. (b) The body weight of mice of the experimental groups (Groups A–E) at different times after MCMV infection.
Mentions: Furthermore, the growth of MCMV-infected mice (Controls AV, BV, and CV), as measured by body weight, was slower than those of the corresponding control groups (Controls A, B, and C) (Figure 2(a)) (F = 9.213, P < 0.05). The growth of mice in group D was also slower than the growth of the uninfected mice of Group E (F = 10.492, P < 0.01) (Figure 2(b)).

Bottom Line: Flow cytometry showed that the number of CD4(+) and CD8(+) cells and the level of IFN- γ decreased significantly in the groups treated with cyclosporin A.Transmission electronic microscopy demonstrated the presence of herpes virus particles.MCMV DNA and glycoprotein B were demonstrated in the epithelial cells of the lung tissue in those animals by in situ hybridization and immunohistochemistry, respectively.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Anhui Medical University, Hefei, Anhui 230032, China.

ABSTRACT
We investigated the effect of murine cytomegalovirus (MCMV) on interstitial pneumonia in transplant recipients in an experimental skin allograft model. Skin transplantation between C57BL/6J and BALB/c mice was performed in the presence or absence of cyclosporin A treatment. Flow cytometry showed that the number of CD4(+) and CD8(+) cells and the level of IFN- γ decreased significantly in the groups treated with cyclosporin A. We either mock-infected or infected the mice with MCMV by intranasal administration and monitored pathophysiological behavior and body weight. The infected mice were sacrificed at different days postinfection for histology, immunohistochemistry, and molecular biological evaluations. Interstitial pneumonitis was observed in positive control groups as well as in experimental group that received cyclosporin A, a skin transplant, and infected with the highest dose of virus (10(5) PFU). Transmission electronic microscopy demonstrated the presence of herpes virus particles. MCMV DNA and glycoprotein B were demonstrated in the epithelial cells of the lung tissue in those animals by in situ hybridization and immunohistochemistry, respectively. Our data demonstrated the establishment of a mouse model of interstitial pneumonitis via MCMV infection after allogeneic skin transplantation.

Show MeSH
Related in: MedlinePlus