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A mouse model of interstitial pneumonitis induced by murine cytomegalovirus infection after allogeneic skin transplantation.

Ni D, Yu H, Zhang W, Gan L, Zhao J, Wang M, Chen J - Biomed Res Int (2013)

Bottom Line: Flow cytometry showed that the number of CD4(+) and CD8(+) cells and the level of IFN- γ decreased significantly in the groups treated with cyclosporin A.Transmission electronic microscopy demonstrated the presence of herpes virus particles.MCMV DNA and glycoprotein B were demonstrated in the epithelial cells of the lung tissue in those animals by in situ hybridization and immunohistochemistry, respectively.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Anhui Medical University, Hefei, Anhui 230032, China.

ABSTRACT
We investigated the effect of murine cytomegalovirus (MCMV) on interstitial pneumonia in transplant recipients in an experimental skin allograft model. Skin transplantation between C57BL/6J and BALB/c mice was performed in the presence or absence of cyclosporin A treatment. Flow cytometry showed that the number of CD4(+) and CD8(+) cells and the level of IFN- γ decreased significantly in the groups treated with cyclosporin A. We either mock-infected or infected the mice with MCMV by intranasal administration and monitored pathophysiological behavior and body weight. The infected mice were sacrificed at different days postinfection for histology, immunohistochemistry, and molecular biological evaluations. Interstitial pneumonitis was observed in positive control groups as well as in experimental group that received cyclosporin A, a skin transplant, and infected with the highest dose of virus (10(5) PFU). Transmission electronic microscopy demonstrated the presence of herpes virus particles. MCMV DNA and glycoprotein B were demonstrated in the epithelial cells of the lung tissue in those animals by in situ hybridization and immunohistochemistry, respectively. Our data demonstrated the establishment of a mouse model of interstitial pneumonitis via MCMV infection after allogeneic skin transplantation.

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Skin transplantation from C57BL/6J mouse to BALB/c mice. (a) Shaved C57BL/6J donor mouse before transplantation. (b) BALB/c recipient mice before transplantation. (c) Recipient mouse at 0.5 d after transplantation. (d) Recipient mouse at 4 d after transplantation.
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fig1: Skin transplantation from C57BL/6J mouse to BALB/c mice. (a) Shaved C57BL/6J donor mouse before transplantation. (b) BALB/c recipient mice before transplantation. (c) Recipient mouse at 0.5 d after transplantation. (d) Recipient mouse at 4 d after transplantation.

Mentions: Skin grafts from 20 C57BL/6J mice were successfully transplanted to 112 BALB/c mice (Figure 1). Around 8-9 days after transplantation, skin grafts on the recipient BALB/c mice began to scab off. In the Control B and Control BV groups, most of the skin grafts showed necrosis because of graft rejection in the absence of CsA. Of the mice in experimental Groups A–E, in which CsA was administered continuously for 14 days, subcutaneous blood vessels under the skin grafts were found in 72 (90%) of the transplanted mice. Additionally, 18 (22.5%) mice showed growen hair on the skin grafts.


A mouse model of interstitial pneumonitis induced by murine cytomegalovirus infection after allogeneic skin transplantation.

Ni D, Yu H, Zhang W, Gan L, Zhao J, Wang M, Chen J - Biomed Res Int (2013)

Skin transplantation from C57BL/6J mouse to BALB/c mice. (a) Shaved C57BL/6J donor mouse before transplantation. (b) BALB/c recipient mice before transplantation. (c) Recipient mouse at 0.5 d after transplantation. (d) Recipient mouse at 4 d after transplantation.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3713605&req=5

fig1: Skin transplantation from C57BL/6J mouse to BALB/c mice. (a) Shaved C57BL/6J donor mouse before transplantation. (b) BALB/c recipient mice before transplantation. (c) Recipient mouse at 0.5 d after transplantation. (d) Recipient mouse at 4 d after transplantation.
Mentions: Skin grafts from 20 C57BL/6J mice were successfully transplanted to 112 BALB/c mice (Figure 1). Around 8-9 days after transplantation, skin grafts on the recipient BALB/c mice began to scab off. In the Control B and Control BV groups, most of the skin grafts showed necrosis because of graft rejection in the absence of CsA. Of the mice in experimental Groups A–E, in which CsA was administered continuously for 14 days, subcutaneous blood vessels under the skin grafts were found in 72 (90%) of the transplanted mice. Additionally, 18 (22.5%) mice showed growen hair on the skin grafts.

Bottom Line: Flow cytometry showed that the number of CD4(+) and CD8(+) cells and the level of IFN- γ decreased significantly in the groups treated with cyclosporin A.Transmission electronic microscopy demonstrated the presence of herpes virus particles.MCMV DNA and glycoprotein B were demonstrated in the epithelial cells of the lung tissue in those animals by in situ hybridization and immunohistochemistry, respectively.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Anhui Medical University, Hefei, Anhui 230032, China.

ABSTRACT
We investigated the effect of murine cytomegalovirus (MCMV) on interstitial pneumonia in transplant recipients in an experimental skin allograft model. Skin transplantation between C57BL/6J and BALB/c mice was performed in the presence or absence of cyclosporin A treatment. Flow cytometry showed that the number of CD4(+) and CD8(+) cells and the level of IFN- γ decreased significantly in the groups treated with cyclosporin A. We either mock-infected or infected the mice with MCMV by intranasal administration and monitored pathophysiological behavior and body weight. The infected mice were sacrificed at different days postinfection for histology, immunohistochemistry, and molecular biological evaluations. Interstitial pneumonitis was observed in positive control groups as well as in experimental group that received cyclosporin A, a skin transplant, and infected with the highest dose of virus (10(5) PFU). Transmission electronic microscopy demonstrated the presence of herpes virus particles. MCMV DNA and glycoprotein B were demonstrated in the epithelial cells of the lung tissue in those animals by in situ hybridization and immunohistochemistry, respectively. Our data demonstrated the establishment of a mouse model of interstitial pneumonitis via MCMV infection after allogeneic skin transplantation.

Show MeSH
Related in: MedlinePlus