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Ovulation and rate of implantation following unilateral ovariectomy in mice.

Bhattacharya K - J Hum Reprod Sci (2013)

Bottom Line: Ovulation and number of implantation were decreased in proportion to single contralateral ovary if the animals were mated within 36 hrs of ULO.Following ULO, the remaining ovary started to compensate within 5(th) to 6(th) day of ULO even during pregnancy.Ovarian histology also showed increased formation of CL which is a marker of rate of ovulation.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Vidyasagar College for Women, University of Calcutta, Kolkata, India.

ABSTRACT

Objectives: Removal of an ovary of any side of the body is called the unilateral ovariectomy (ULO). ULO models are used to analyze the existence of functional and physiological asymmetries between the ovaries, including the ovum implantation.

Materials and methods: ULO was done in mice without any specification on their stages of the estrous cycle. The animals were mated between 3(rd) and 15(th) day after ULO to observe the consequence of ULO on ovulation and ovum implantation.

Results: Ovulation and number of implantation were decreased in proportion to single contralateral ovary if the animals were mated within 36 hrs of ULO. It resulted in increased weight of contralateral ovary due to hypertrophy, and also in compensatory ovulation, formation of corpus luteum (CL) that reached a peak after 14(th) and 15(th) day of ULO (sacrificed on 5(th) day of pregnancy i.e., 19(th) and 20(th) day of ULO) compared to animals undergone sham-surgery. Numbers of implantations were also increased progressively on the contralateral ovarian side and get to a peak after similar days of compensation.

Discussion and conclusion: Following ULO, the remaining ovary started to compensate within 5(th) to 6(th) day of ULO even during pregnancy. Ovarian histology also showed increased formation of CL which is a marker of rate of ovulation.

No MeSH data available.


Related in: MedlinePlus

Implantation sites after unilateral ovariectomy (a) showing two implantation sites, 3 days after ULO, (b) showing six implantation sites 4, 5, and 6 days after ULO, (c) showing 6 implantation sites 4, 5, and 6 days after ULO, (d) showing eight implantation sites 14 days after ULO, (e) showing nine implantation sites 15 days after ULO, (f) showing 10 implantation sites of sham operated mice
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Figure 2: Implantation sites after unilateral ovariectomy (a) showing two implantation sites, 3 days after ULO, (b) showing six implantation sites 4, 5, and 6 days after ULO, (c) showing 6 implantation sites 4, 5, and 6 days after ULO, (d) showing eight implantation sites 14 days after ULO, (e) showing nine implantation sites 15 days after ULO, (f) showing 10 implantation sites of sham operated mice

Mentions: The contralateral uterine horn of unilaterally ovariectomized mice contains blastocysts after getting pregnant, having the increase in length during the early stages of implantation. At the same time, the implantation swellings of blastocysts were visible macroscopically before the implantation, after the administration of pontamine blue injection [Figure 2]. It is concluded that growth in length of the uterus occurs during the implantation reaction and this may assist in the accommodation of blastocysts along the length of the uterus.[10] A single horn of the mice uterus is provided for the development of as many as 12 young pups, after 3 weeks[11] and the single remaining ovary in unilaterally ovariectomized animals (assuming the number of corpora lutea to be an index of ovulation) produces as many ova as both ovaries in normal females.


Ovulation and rate of implantation following unilateral ovariectomy in mice.

Bhattacharya K - J Hum Reprod Sci (2013)

Implantation sites after unilateral ovariectomy (a) showing two implantation sites, 3 days after ULO, (b) showing six implantation sites 4, 5, and 6 days after ULO, (c) showing 6 implantation sites 4, 5, and 6 days after ULO, (d) showing eight implantation sites 14 days after ULO, (e) showing nine implantation sites 15 days after ULO, (f) showing 10 implantation sites of sham operated mice
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3713577&req=5

Figure 2: Implantation sites after unilateral ovariectomy (a) showing two implantation sites, 3 days after ULO, (b) showing six implantation sites 4, 5, and 6 days after ULO, (c) showing 6 implantation sites 4, 5, and 6 days after ULO, (d) showing eight implantation sites 14 days after ULO, (e) showing nine implantation sites 15 days after ULO, (f) showing 10 implantation sites of sham operated mice
Mentions: The contralateral uterine horn of unilaterally ovariectomized mice contains blastocysts after getting pregnant, having the increase in length during the early stages of implantation. At the same time, the implantation swellings of blastocysts were visible macroscopically before the implantation, after the administration of pontamine blue injection [Figure 2]. It is concluded that growth in length of the uterus occurs during the implantation reaction and this may assist in the accommodation of blastocysts along the length of the uterus.[10] A single horn of the mice uterus is provided for the development of as many as 12 young pups, after 3 weeks[11] and the single remaining ovary in unilaterally ovariectomized animals (assuming the number of corpora lutea to be an index of ovulation) produces as many ova as both ovaries in normal females.

Bottom Line: Ovulation and number of implantation were decreased in proportion to single contralateral ovary if the animals were mated within 36 hrs of ULO.Following ULO, the remaining ovary started to compensate within 5(th) to 6(th) day of ULO even during pregnancy.Ovarian histology also showed increased formation of CL which is a marker of rate of ovulation.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Vidyasagar College for Women, University of Calcutta, Kolkata, India.

ABSTRACT

Objectives: Removal of an ovary of any side of the body is called the unilateral ovariectomy (ULO). ULO models are used to analyze the existence of functional and physiological asymmetries between the ovaries, including the ovum implantation.

Materials and methods: ULO was done in mice without any specification on their stages of the estrous cycle. The animals were mated between 3(rd) and 15(th) day after ULO to observe the consequence of ULO on ovulation and ovum implantation.

Results: Ovulation and number of implantation were decreased in proportion to single contralateral ovary if the animals were mated within 36 hrs of ULO. It resulted in increased weight of contralateral ovary due to hypertrophy, and also in compensatory ovulation, formation of corpus luteum (CL) that reached a peak after 14(th) and 15(th) day of ULO (sacrificed on 5(th) day of pregnancy i.e., 19(th) and 20(th) day of ULO) compared to animals undergone sham-surgery. Numbers of implantations were also increased progressively on the contralateral ovarian side and get to a peak after similar days of compensation.

Discussion and conclusion: Following ULO, the remaining ovary started to compensate within 5(th) to 6(th) day of ULO even during pregnancy. Ovarian histology also showed increased formation of CL which is a marker of rate of ovulation.

No MeSH data available.


Related in: MedlinePlus