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Nicotine alters male reproductive hormones in male albino rats: The role of cessation.

Oyeyipo IP, Raji Y, Bolarinwa AF - J Hum Reprod Sci (2013)

Bottom Line: Results showed that nicotine administration significantly decreased (P < 0.05) testosterone in the low and high treated groups and FSH in the high dose treated group when compared with the control group.There was a significant increase (P < 0.05) in mean LH and prolactin level in the high dose treated group when compared with the control.It is plausible that the decreased testosterone level is associated with testicular dysfunction rather than a pituitary disorder.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, College of Health Sciences, Osun State University, Osogbo, Osun State, Nigeria ; Laboratory for Reproductive Physiology and Developmental Programming, Department of Physiology, College of Medicine, University of Ibadan, Ibadan, Oyo State, Nigeria.

ABSTRACT

Objectives: The use of nicotine through smoking remains a serious health problem. It has been associated with reduced fertility, although the mechanism responsible is still unclear. The present study was designed to investigate whether nicotine-induced infertility is associated with altered male reproductive hormones in male albino rats.

Materials and methods: Forty male rats were divided equally into five groups and treated orally for thirty days. Group I, which served as the control received 0.2 ml/kg normal saline, Group II and III received 0.5 mg/kg (low dose) and 1.0 mg/kg (high dose) body weight of nicotine, respectively. The fourth and fifth groups were gavaged with 0.5 mg/kg and 1.0 mg/kg body weight of nicotine but were left untreated for another 30 days. These groups served as the recovery groups. Serum was analyzed for testosterone, luteinizing hormone (LH), follicle stimulating hormones (FSH), and prolactin using radioimmunoassay.

Results: Results showed that nicotine administration significantly decreased (P < 0.05) testosterone in the low and high treated groups and FSH in the high dose treated group when compared with the control group. There was a significant increase (P < 0.05) in mean LH and prolactin level in the high dose treated group when compared with the control. However, the values of the recovery groups were comparable with the control.

Conclusion: The findings in this study suggest that nicotine administration is associated with distorted reproductive hormones in male rats although ameliorated by nicotine cessation. It is plausible that the decreased testosterone level is associated with testicular dysfunction rather than a pituitary disorder.

No MeSH data available.


Related in: MedlinePlus

Effect of nicotine on serum follicle stimulating hormone level in male rats. Values are expressed as means ± S.E.M of eight rats per group. Bar carrying the asterisk sign on each parameter are significantly different at P < 0.05
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Figure 3: Effect of nicotine on serum follicle stimulating hormone level in male rats. Values are expressed as means ± S.E.M of eight rats per group. Bar carrying the asterisk sign on each parameter are significantly different at P < 0.05

Mentions: The mean FSH level of rats that received 1.0 mg/kg BW nicotine daily for four weeks was significantly decreased (P < 0.05) when compared with the control group. However, 0.5 mg/kg BW and the recovery groups showed an insignificant decrease (P > 0.05) in the mean serum FSH level when compared with their control counterpart as shown in Figure 3.


Nicotine alters male reproductive hormones in male albino rats: The role of cessation.

Oyeyipo IP, Raji Y, Bolarinwa AF - J Hum Reprod Sci (2013)

Effect of nicotine on serum follicle stimulating hormone level in male rats. Values are expressed as means ± S.E.M of eight rats per group. Bar carrying the asterisk sign on each parameter are significantly different at P < 0.05
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3713576&req=5

Figure 3: Effect of nicotine on serum follicle stimulating hormone level in male rats. Values are expressed as means ± S.E.M of eight rats per group. Bar carrying the asterisk sign on each parameter are significantly different at P < 0.05
Mentions: The mean FSH level of rats that received 1.0 mg/kg BW nicotine daily for four weeks was significantly decreased (P < 0.05) when compared with the control group. However, 0.5 mg/kg BW and the recovery groups showed an insignificant decrease (P > 0.05) in the mean serum FSH level when compared with their control counterpart as shown in Figure 3.

Bottom Line: Results showed that nicotine administration significantly decreased (P < 0.05) testosterone in the low and high treated groups and FSH in the high dose treated group when compared with the control group.There was a significant increase (P < 0.05) in mean LH and prolactin level in the high dose treated group when compared with the control.It is plausible that the decreased testosterone level is associated with testicular dysfunction rather than a pituitary disorder.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, College of Health Sciences, Osun State University, Osogbo, Osun State, Nigeria ; Laboratory for Reproductive Physiology and Developmental Programming, Department of Physiology, College of Medicine, University of Ibadan, Ibadan, Oyo State, Nigeria.

ABSTRACT

Objectives: The use of nicotine through smoking remains a serious health problem. It has been associated with reduced fertility, although the mechanism responsible is still unclear. The present study was designed to investigate whether nicotine-induced infertility is associated with altered male reproductive hormones in male albino rats.

Materials and methods: Forty male rats were divided equally into five groups and treated orally for thirty days. Group I, which served as the control received 0.2 ml/kg normal saline, Group II and III received 0.5 mg/kg (low dose) and 1.0 mg/kg (high dose) body weight of nicotine, respectively. The fourth and fifth groups were gavaged with 0.5 mg/kg and 1.0 mg/kg body weight of nicotine but were left untreated for another 30 days. These groups served as the recovery groups. Serum was analyzed for testosterone, luteinizing hormone (LH), follicle stimulating hormones (FSH), and prolactin using radioimmunoassay.

Results: Results showed that nicotine administration significantly decreased (P < 0.05) testosterone in the low and high treated groups and FSH in the high dose treated group when compared with the control group. There was a significant increase (P < 0.05) in mean LH and prolactin level in the high dose treated group when compared with the control. However, the values of the recovery groups were comparable with the control.

Conclusion: The findings in this study suggest that nicotine administration is associated with distorted reproductive hormones in male rats although ameliorated by nicotine cessation. It is plausible that the decreased testosterone level is associated with testicular dysfunction rather than a pituitary disorder.

No MeSH data available.


Related in: MedlinePlus