Limits...
Does centrifugation and semen processing with swim up at 37°C yield sperm with better DNA integrity compared to centrifugation and processing at room temperature?

Repalle D, Chittawar PB, Bhandari S, Joshi G, Paranjape M, Joshi C - J Hum Reprod Sci (2013)

Bottom Line: Prospective pilot study.Normozoospermic men (n = 50) undergoing diagnostic semen analysis.Paired t-test was used for calculating the sperm DNA integrity difference between post wash at RT and 37°C.

View Article: PubMed Central - PubMed

Affiliation: Department of Reproductive Medicine, Mohak Hi-tech Speciality Laparoscopy and Infertility Centre, Sri Aurobindo Institute of Medical Sciences, Indore, Madhya Pradesh, India.

ABSTRACT

Aim: To evaluate whether semen processing at 37°C yield sperm with better DNA integrity compared to centrifugation and processing at room temperature (RT) by swim-up method.

Settings: This study was done at tertiary care center attached to Reproductive Medicine Unit and Medical College.

Design: Prospective pilot study.

Patients: Normozoospermic men (n = 50) undergoing diagnostic semen analysis.

Materials and methods: Normozoospermic samples (World Health Organization, 2010 criteria) after analysis was divided into two aliquots (0.5 mL each); one was processed at 37°C and the other at RT by swim-up method. DNA fragmentation of both samples post wash was calculated by acridine orange method.

Statistical analysis used: The values of sperm DNA fragmentation were represented as mean and standard error (mean ± SEM) of the mean. Paired t-test was used for calculating the sperm DNA integrity difference between post wash at RT and 37°C.

Results: Statistically significant difference was not observed in post wash sperm DNA fragmentation values at 37°C compared to RT.

Conclusion: Our data represents that there was no significant difference in sperm DNA fragmentation values of samples processed at 37°C and at RT. Hence, sperm processing at 37°C does not yield sperm with better DNA integrity compared to centrifugation and processing at RT.

No MeSH data available.


Related in: MedlinePlus

Effect of temperature on sperm DNA fragmentation in post wash at room temperature and 37°C
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3713571&req=5

Figure 1: Effect of temperature on sperm DNA fragmentation in post wash at room temperature and 37°C

Mentions: In our study, statistically significant difference was not observed in sperm DNA fragmentation values in postwash at 37°C compared to RT (t = 1.6151, P = 0.1127) [Figure 1].


Does centrifugation and semen processing with swim up at 37°C yield sperm with better DNA integrity compared to centrifugation and processing at room temperature?

Repalle D, Chittawar PB, Bhandari S, Joshi G, Paranjape M, Joshi C - J Hum Reprod Sci (2013)

Effect of temperature on sperm DNA fragmentation in post wash at room temperature and 37°C
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3713571&req=5

Figure 1: Effect of temperature on sperm DNA fragmentation in post wash at room temperature and 37°C
Mentions: In our study, statistically significant difference was not observed in sperm DNA fragmentation values in postwash at 37°C compared to RT (t = 1.6151, P = 0.1127) [Figure 1].

Bottom Line: Prospective pilot study.Normozoospermic men (n = 50) undergoing diagnostic semen analysis.Paired t-test was used for calculating the sperm DNA integrity difference between post wash at RT and 37°C.

View Article: PubMed Central - PubMed

Affiliation: Department of Reproductive Medicine, Mohak Hi-tech Speciality Laparoscopy and Infertility Centre, Sri Aurobindo Institute of Medical Sciences, Indore, Madhya Pradesh, India.

ABSTRACT

Aim: To evaluate whether semen processing at 37°C yield sperm with better DNA integrity compared to centrifugation and processing at room temperature (RT) by swim-up method.

Settings: This study was done at tertiary care center attached to Reproductive Medicine Unit and Medical College.

Design: Prospective pilot study.

Patients: Normozoospermic men (n = 50) undergoing diagnostic semen analysis.

Materials and methods: Normozoospermic samples (World Health Organization, 2010 criteria) after analysis was divided into two aliquots (0.5 mL each); one was processed at 37°C and the other at RT by swim-up method. DNA fragmentation of both samples post wash was calculated by acridine orange method.

Statistical analysis used: The values of sperm DNA fragmentation were represented as mean and standard error (mean ± SEM) of the mean. Paired t-test was used for calculating the sperm DNA integrity difference between post wash at RT and 37°C.

Results: Statistically significant difference was not observed in post wash sperm DNA fragmentation values at 37°C compared to RT.

Conclusion: Our data represents that there was no significant difference in sperm DNA fragmentation values of samples processed at 37°C and at RT. Hence, sperm processing at 37°C does not yield sperm with better DNA integrity compared to centrifugation and processing at RT.

No MeSH data available.


Related in: MedlinePlus