Limits...
Structure-based discovery of fiber-binding compounds that reduce the cytotoxicity of amyloid beta.

Jiang L, Liu C, Leibly D, Landau M, Zhao M, Hughes MP, Eisenberg DS - Elife (2013)

Bottom Line: While structure-based discovery of compounds has been effective in combating numerous infectious and metabolic diseases, ignorance of amyloid structure has hindered similar approaches to amyloid disease.Although these compounds bind to Aβ fibers, they do not reduce fiber formation of Aβ.Structure-activity relationship studies of the fiber-binding compounds and their derivatives suggest that compound binding increases fiber stability and decreases fiber toxicity, perhaps by shifting the equilibrium of Aβ from oligomers to fibers.

View Article: PubMed Central - PubMed

Affiliation: Departments of Chemistry and Biochemistry and Biological Chemistry , Howard Hughes Medical Institute, UCLA-DOE Institute for Genomics and Proteomics, University of California, Los Angeles , Los Angeles , United States.

ABSTRACT
Amyloid protein aggregates are associated with dozens of devastating diseases including Alzheimer's, Parkinson's, ALS, and diabetes type 2. While structure-based discovery of compounds has been effective in combating numerous infectious and metabolic diseases, ignorance of amyloid structure has hindered similar approaches to amyloid disease. Here we show that knowledge of the atomic structure of one of the adhesive, steric-zipper segments of the amyloid-beta (Aβ) protein of Alzheimer's disease, when coupled with computational methods, identifies eight diverse but mainly flat compounds and three compound derivatives that reduce Aβ cytotoxicity against mammalian cells by up to 90%. Although these compounds bind to Aβ fibers, they do not reduce fiber formation of Aβ. Structure-activity relationship studies of the fiber-binding compounds and their derivatives suggest that compound binding increases fiber stability and decreases fiber toxicity, perhaps by shifting the equilibrium of Aβ from oligomers to fibers. DOI:http://dx.doi.org/10.7554/eLife.00857.001.

Show MeSH

Related in: MedlinePlus

Diversified chemical structures of 8 active BAF compounds that reduce Aβ toxicity.Orange G in an orange box is also displayed for comparison.DOI:http://dx.doi.org/10.7554/eLife.00857.017
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3713518&req=5

fig4: Diversified chemical structures of 8 active BAF compounds that reduce Aβ toxicity.Orange G in an orange box is also displayed for comparison.DOI:http://dx.doi.org/10.7554/eLife.00857.017

Mentions: Having identified compounds that bind Aβ fibers, by a structure-based procedure, we tested their effects on the cytotoxicity of Aβ1–42 fiber against two mammalian cell lines: PC12 and HeLa (Figure 3). Five BAFs—1,4,8,11, and 12—in the initial cycle and three additional BAFs—26, 30, and 31—from the second cycle, with diversified chemical structures shown in Figure 4, significantly increased both PC12 and HeLa cell survival after 24 hr incubation with Aβ1–42 (0.5 µM) at concentration of 2.5 µM, while the BAFs alone had little or no effect on cell survival (Figure 3—figure supplement 1). Three BAFs—11, 26, and 31—showed clear dose-response profiles in their protection of both PC12 and HeLa cells (Figure 3B). Among them, the two best BAFs—26 and 31—were tested and did not affect the cytotoxicity of amyloid fibers other than Aβ (Figure 3—figure supplement 2). Although all of these BAFs provide protection against Aβ toxicity, none diminish the amount of Aβ fibers in electron micrographs (Figure 3C).10.7554/eLife.00857.013Figure 3.Experimental characterization of compounds that bind to amyloid fibers.


Structure-based discovery of fiber-binding compounds that reduce the cytotoxicity of amyloid beta.

Jiang L, Liu C, Leibly D, Landau M, Zhao M, Hughes MP, Eisenberg DS - Elife (2013)

Diversified chemical structures of 8 active BAF compounds that reduce Aβ toxicity.Orange G in an orange box is also displayed for comparison.DOI:http://dx.doi.org/10.7554/eLife.00857.017
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3713518&req=5

fig4: Diversified chemical structures of 8 active BAF compounds that reduce Aβ toxicity.Orange G in an orange box is also displayed for comparison.DOI:http://dx.doi.org/10.7554/eLife.00857.017
Mentions: Having identified compounds that bind Aβ fibers, by a structure-based procedure, we tested their effects on the cytotoxicity of Aβ1–42 fiber against two mammalian cell lines: PC12 and HeLa (Figure 3). Five BAFs—1,4,8,11, and 12—in the initial cycle and three additional BAFs—26, 30, and 31—from the second cycle, with diversified chemical structures shown in Figure 4, significantly increased both PC12 and HeLa cell survival after 24 hr incubation with Aβ1–42 (0.5 µM) at concentration of 2.5 µM, while the BAFs alone had little or no effect on cell survival (Figure 3—figure supplement 1). Three BAFs—11, 26, and 31—showed clear dose-response profiles in their protection of both PC12 and HeLa cells (Figure 3B). Among them, the two best BAFs—26 and 31—were tested and did not affect the cytotoxicity of amyloid fibers other than Aβ (Figure 3—figure supplement 2). Although all of these BAFs provide protection against Aβ toxicity, none diminish the amount of Aβ fibers in electron micrographs (Figure 3C).10.7554/eLife.00857.013Figure 3.Experimental characterization of compounds that bind to amyloid fibers.

Bottom Line: While structure-based discovery of compounds has been effective in combating numerous infectious and metabolic diseases, ignorance of amyloid structure has hindered similar approaches to amyloid disease.Although these compounds bind to Aβ fibers, they do not reduce fiber formation of Aβ.Structure-activity relationship studies of the fiber-binding compounds and their derivatives suggest that compound binding increases fiber stability and decreases fiber toxicity, perhaps by shifting the equilibrium of Aβ from oligomers to fibers.

View Article: PubMed Central - PubMed

Affiliation: Departments of Chemistry and Biochemistry and Biological Chemistry , Howard Hughes Medical Institute, UCLA-DOE Institute for Genomics and Proteomics, University of California, Los Angeles , Los Angeles , United States.

ABSTRACT
Amyloid protein aggregates are associated with dozens of devastating diseases including Alzheimer's, Parkinson's, ALS, and diabetes type 2. While structure-based discovery of compounds has been effective in combating numerous infectious and metabolic diseases, ignorance of amyloid structure has hindered similar approaches to amyloid disease. Here we show that knowledge of the atomic structure of one of the adhesive, steric-zipper segments of the amyloid-beta (Aβ) protein of Alzheimer's disease, when coupled with computational methods, identifies eight diverse but mainly flat compounds and three compound derivatives that reduce Aβ cytotoxicity against mammalian cells by up to 90%. Although these compounds bind to Aβ fibers, they do not reduce fiber formation of Aβ. Structure-activity relationship studies of the fiber-binding compounds and their derivatives suggest that compound binding increases fiber stability and decreases fiber toxicity, perhaps by shifting the equilibrium of Aβ from oligomers to fibers. DOI:http://dx.doi.org/10.7554/eLife.00857.001.

Show MeSH
Related in: MedlinePlus