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Dynamic changes in glycosylation and glycan composition of serum FSH and LH during natural ovarian stimulation.

Wide L, Eriksson K - Ups. J. Med. Sci. (2013)

Bottom Line: The mean numbers of SA and SU residues per molecule of FSH and LH in serum showed four different patterns during the cycle, all with highly significant (P < 0.0001) differences between levels at different phases of the cycle.The pattern of SA residues on FSH was 'M'-shaped, and that of SU on LH 'V'-shaped.This new information on the FSH and LH molecular structures may lead to more successful mono-ovulatory treatment regimens for ovulation induction in anovulatory women.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Sciences, Uppsala University, Clinical Chemistry, University Hospital, SE 751 85 Uppsala, Sweden. leif.wide@medsci.uu.se

ABSTRACT

Background: Glycosylation and glycan composition are of fundamental importance for the biological properties of FSH and LH. The aim of this study was to determine the glycosylation, sialylation, and sulfonation of serum FSH and LH throughout the normal menstrual cycle.

Methods: Serum samples were collected from 79 healthy women with regular menstrual cycles. The mean numbers of anionic monosaccharide (AMS), sialic acid (SA), and sulfonated N-acetylgalactosamine (SU) residues per FSH and LH molecule were estimated for all sera with methods based on electrophoreses, neuraminidase treatments, and fluoroimmunoassays of the gonadotrophins.

Results: Di-glycosylated glycoforms (FSHdi, LHdi) were detected in serum in addition to tetra-glycosylated FSH (FSHtetra) and tri-glycosylated LH (LHtri). FSHdi exhibited two peaks: one on day 5 to 7 and one, more pronounced, at midcycle. FSHtetra plateaued at a high concentration from day 5 to 15, without a midcycle peak. There were lower concentrations of LHdi than LHtri, except at midcycle when the opposite occurred. The mean numbers of SA and SU residues per molecule of FSH and LH in serum showed four different patterns during the cycle, all with highly significant (P < 0.0001) differences between levels at different phases of the cycle. The pattern of SA residues on FSH was 'M'-shaped, and that of SU on LH 'V'-shaped.

Conclusion: Serum FSH and LH governing the natural ovarian stimulation process exhibited dynamic changes of glycosylation and glycan composition. This new information on the FSH and LH molecular structures may lead to more successful mono-ovulatory treatment regimens for ovulation induction in anovulatory women.

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Related in: MedlinePlus

A: Concentrations of FSH and LH in serum of 79 women with a normal menstrual cycle, plotted as geometric mean ± SEM values. B: The number of anionic monosaccharide (AMS) residues per molecule of FSH and LH in the same serum samples, plotted as three-day mean ± SEM values. Data in this figure and in Figures 2,3,4,5 have been plotted as three-day moving mean values starting during the last days of the previous cycle and with the first day of the cycle indicated by a vertical hatched bar.
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Figure 1: A: Concentrations of FSH and LH in serum of 79 women with a normal menstrual cycle, plotted as geometric mean ± SEM values. B: The number of anionic monosaccharide (AMS) residues per molecule of FSH and LH in the same serum samples, plotted as three-day mean ± SEM values. Data in this figure and in Figures 2,3,4,5 have been plotted as three-day moving mean values starting during the last days of the previous cycle and with the first day of the cycle indicated by a vertical hatched bar.

Mentions: There was a rise of serum FSH during the early follicular phase, from day 27 in the previous cycle to day 5 (Figure 1, left panel). The midcycle LH and FSH surges coincided and occurred around day 14. Before that the serum LH concentration increased gradually from day 8 to day 12. During the same period the FSH concentration remained at a slightly decreased plateau level. The midcycle surges of FSH and LH were followed by rapid decreases of the serum concentrations of both gonadotrophins.


Dynamic changes in glycosylation and glycan composition of serum FSH and LH during natural ovarian stimulation.

Wide L, Eriksson K - Ups. J. Med. Sci. (2013)

A: Concentrations of FSH and LH in serum of 79 women with a normal menstrual cycle, plotted as geometric mean ± SEM values. B: The number of anionic monosaccharide (AMS) residues per molecule of FSH and LH in the same serum samples, plotted as three-day mean ± SEM values. Data in this figure and in Figures 2,3,4,5 have been plotted as three-day moving mean values starting during the last days of the previous cycle and with the first day of the cycle indicated by a vertical hatched bar.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3713380&req=5

Figure 1: A: Concentrations of FSH and LH in serum of 79 women with a normal menstrual cycle, plotted as geometric mean ± SEM values. B: The number of anionic monosaccharide (AMS) residues per molecule of FSH and LH in the same serum samples, plotted as three-day mean ± SEM values. Data in this figure and in Figures 2,3,4,5 have been plotted as three-day moving mean values starting during the last days of the previous cycle and with the first day of the cycle indicated by a vertical hatched bar.
Mentions: There was a rise of serum FSH during the early follicular phase, from day 27 in the previous cycle to day 5 (Figure 1, left panel). The midcycle LH and FSH surges coincided and occurred around day 14. Before that the serum LH concentration increased gradually from day 8 to day 12. During the same period the FSH concentration remained at a slightly decreased plateau level. The midcycle surges of FSH and LH were followed by rapid decreases of the serum concentrations of both gonadotrophins.

Bottom Line: The mean numbers of SA and SU residues per molecule of FSH and LH in serum showed four different patterns during the cycle, all with highly significant (P < 0.0001) differences between levels at different phases of the cycle.The pattern of SA residues on FSH was 'M'-shaped, and that of SU on LH 'V'-shaped.This new information on the FSH and LH molecular structures may lead to more successful mono-ovulatory treatment regimens for ovulation induction in anovulatory women.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Sciences, Uppsala University, Clinical Chemistry, University Hospital, SE 751 85 Uppsala, Sweden. leif.wide@medsci.uu.se

ABSTRACT

Background: Glycosylation and glycan composition are of fundamental importance for the biological properties of FSH and LH. The aim of this study was to determine the glycosylation, sialylation, and sulfonation of serum FSH and LH throughout the normal menstrual cycle.

Methods: Serum samples were collected from 79 healthy women with regular menstrual cycles. The mean numbers of anionic monosaccharide (AMS), sialic acid (SA), and sulfonated N-acetylgalactosamine (SU) residues per FSH and LH molecule were estimated for all sera with methods based on electrophoreses, neuraminidase treatments, and fluoroimmunoassays of the gonadotrophins.

Results: Di-glycosylated glycoforms (FSHdi, LHdi) were detected in serum in addition to tetra-glycosylated FSH (FSHtetra) and tri-glycosylated LH (LHtri). FSHdi exhibited two peaks: one on day 5 to 7 and one, more pronounced, at midcycle. FSHtetra plateaued at a high concentration from day 5 to 15, without a midcycle peak. There were lower concentrations of LHdi than LHtri, except at midcycle when the opposite occurred. The mean numbers of SA and SU residues per molecule of FSH and LH in serum showed four different patterns during the cycle, all with highly significant (P < 0.0001) differences between levels at different phases of the cycle. The pattern of SA residues on FSH was 'M'-shaped, and that of SU on LH 'V'-shaped.

Conclusion: Serum FSH and LH governing the natural ovarian stimulation process exhibited dynamic changes of glycosylation and glycan composition. This new information on the FSH and LH molecular structures may lead to more successful mono-ovulatory treatment regimens for ovulation induction in anovulatory women.

Show MeSH
Related in: MedlinePlus