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Effects of Moxibustion Temperature on Blood Cholesterol Level in a Mice Model of Acute Hyperlipidemia: Role of TRPV1.

Wang GY, Wang LL, Xu B, Zhang JB, Jiang JF - Evid Based Complement Alternat Med (2013)

Bottom Line: Results.In WT mice, moxibustion caused a decrease in blood cholesterol level and upregulation of TRPV1 at the mRNA level, which was significantly greater in the 46°C group.Conclusions.

View Article: PubMed Central - PubMed

Affiliation: The Second Clinical Medical College, Nanjing University of Traditional Chinese Medicine, Nanjing 210046, China ; Department of Acupuncture and Moxibustion, Zhongda Hospital Affiliated to Southeast University, Nanjing 210029, China.

ABSTRACT
Objectives. To compare the effects of moxibustion at two different temperatures (38°C and 46°C) on the blood cholesterol level in a mice model of acute hyperlipidemia, to detect the different expression levels of transient receptor potential vanilloid subfamily 1 (TRPV1) in the dorsal root ganglions of the wild mice, and to explore the correlation between TRPV1 and moxibustion's cholesterol-lowering effects. Method. Two different mice models were used: C57BL/6J wild type (WT) and TRPV1 gene knockout (TRPV1-/-). Each model was randomly divided into control group and model group with three subgroups after acute hyperlipidemia was established: model control group, 38°C moxibustion group, and 46°C moxibustion group. The mice in 38°C group and 46°C group were subject to moxibustion. After the therapy, the cholesterol concentration in serum was measured, and the expression of TRPV1 was quantified. Results. In WT mice, moxibustion caused a decrease in blood cholesterol level and upregulation of TRPV1 at the mRNA level, which was significantly greater in the 46°C group. In contrast, in TRPV1-/- mice, the differences of cholesterol-lowering effects of moxibustion were lost. Conclusions. Temperature is one of the important factors affecting the effects of moxibustion, and the cholesterol -lowering effect of moxibustion is related to the activation of TRPV1.

No MeSH data available.


Related in: MedlinePlus

Electrophoretograms of four PCR experiments on WT mice and TRPV1−/− mice.
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fig6: Electrophoretograms of four PCR experiments on WT mice and TRPV1−/− mice.

Mentions: Figure 6 proved again that TRPV1 in the TRPV1−/− mice has been successfully knocked out.


Effects of Moxibustion Temperature on Blood Cholesterol Level in a Mice Model of Acute Hyperlipidemia: Role of TRPV1.

Wang GY, Wang LL, Xu B, Zhang JB, Jiang JF - Evid Based Complement Alternat Med (2013)

Electrophoretograms of four PCR experiments on WT mice and TRPV1−/− mice.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3713370&req=5

fig6: Electrophoretograms of four PCR experiments on WT mice and TRPV1−/− mice.
Mentions: Figure 6 proved again that TRPV1 in the TRPV1−/− mice has been successfully knocked out.

Bottom Line: Results.In WT mice, moxibustion caused a decrease in blood cholesterol level and upregulation of TRPV1 at the mRNA level, which was significantly greater in the 46°C group.Conclusions.

View Article: PubMed Central - PubMed

Affiliation: The Second Clinical Medical College, Nanjing University of Traditional Chinese Medicine, Nanjing 210046, China ; Department of Acupuncture and Moxibustion, Zhongda Hospital Affiliated to Southeast University, Nanjing 210029, China.

ABSTRACT
Objectives. To compare the effects of moxibustion at two different temperatures (38°C and 46°C) on the blood cholesterol level in a mice model of acute hyperlipidemia, to detect the different expression levels of transient receptor potential vanilloid subfamily 1 (TRPV1) in the dorsal root ganglions of the wild mice, and to explore the correlation between TRPV1 and moxibustion's cholesterol-lowering effects. Method. Two different mice models were used: C57BL/6J wild type (WT) and TRPV1 gene knockout (TRPV1-/-). Each model was randomly divided into control group and model group with three subgroups after acute hyperlipidemia was established: model control group, 38°C moxibustion group, and 46°C moxibustion group. The mice in 38°C group and 46°C group were subject to moxibustion. After the therapy, the cholesterol concentration in serum was measured, and the expression of TRPV1 was quantified. Results. In WT mice, moxibustion caused a decrease in blood cholesterol level and upregulation of TRPV1 at the mRNA level, which was significantly greater in the 46°C group. In contrast, in TRPV1-/- mice, the differences of cholesterol-lowering effects of moxibustion were lost. Conclusions. Temperature is one of the important factors affecting the effects of moxibustion, and the cholesterol -lowering effect of moxibustion is related to the activation of TRPV1.

No MeSH data available.


Related in: MedlinePlus