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Isoflavonoid-Rich Flemingia macrophylla Extract Attenuates UVB-Induced Skin Damage by Scavenging Reactive Oxygen Species and Inhibiting MAP Kinase and MMP Expression.

Chiang HM, Chiu HH, Liao ST, Chen YT, Chang HC, Wen KC - Evid Based Complement Alternat Med (2013)

Bottom Line: The IC50 values were 2.1  μ g/mL for DPPH radical scavenging ability, 366.8  μ g/mL for superoxide anion scavenging ability, 178.9  μ g/mL for hydrogen peroxide scavenging ability, and 230.9  μ g/mL for hydroxyl radical scavenging ability.In human fibroblasts, FME at 10  μ g/mL was shown to be a potent scavenger of UV-induced reactive oxygen species (ROS).The antioxidant and anti-photoaging properties of FME make it an ideal anti-intrinsic aging and anti-photoaging agent.

View Article: PubMed Central - PubMed

Affiliation: Department of Cosmeceutics, China Medical University, Taichung 404, Taiwan.

ABSTRACT
In this study, we investigated the antioxidant activity and anti-photoaging properties of an extract of Flemingia macrophylla, a plant rich in isoflavonoid content. Pretreatment of fibroblasts with Flemingia macrophylla extract (FME) inhibited elastase activity, promoted the protein expression of type I procollagen, and attenuated the phosphorylation of mitogen-activated protein (MAP) kinase and the protein expression of matrix-metalloproteinase- (MMP-) 1, 3, and 9. The IC50 values were 2.1  μ g/mL for DPPH radical scavenging ability, 366.8  μ g/mL for superoxide anion scavenging ability, 178.9  μ g/mL for hydrogen peroxide scavenging ability, and 230.9  μ g/mL for hydroxyl radical scavenging ability. Also, exposure of erythrocytes to various concentrations of FME (50-500  μ g/mL) resulted in a dose- and time-dependent inhibition of AAPH-induced hemolysis. In human fibroblasts, FME at 10  μ g/mL was shown to be a potent scavenger of UV-induced reactive oxygen species (ROS). The antioxidant and anti-photoaging properties of FME make it an ideal anti-intrinsic aging and anti-photoaging agent.

No MeSH data available.


Related in: MedlinePlus

Effect of FME on the UV-induced (a) type I procollagen expression in human fibroblasts; (b) MMP-1, MMP-3, and MMP-9 expression in human fibroblasts; (c) MMP-9 by gelatin zymography in the culture medium of human fibroblasts; and (d) MAP kinases expression in human fibroblasts.
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Related In: Results  -  Collection


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fig6: Effect of FME on the UV-induced (a) type I procollagen expression in human fibroblasts; (b) MMP-1, MMP-3, and MMP-9 expression in human fibroblasts; (c) MMP-9 by gelatin zymography in the culture medium of human fibroblasts; and (d) MAP kinases expression in human fibroblasts.

Mentions: Fibroblasts were treated with FME (5–50 μg/mL) for 24 h after exposure to UVB (80 mJ/cm2) and the levels of type I procollagen were measured using Western blot. EGCG was used as the positive control. FME treatment (≥10 μg/mL) resulted in a significant increase in the production of type I procollagen relative to that of control cells (Figure 6(a)).


Isoflavonoid-Rich Flemingia macrophylla Extract Attenuates UVB-Induced Skin Damage by Scavenging Reactive Oxygen Species and Inhibiting MAP Kinase and MMP Expression.

Chiang HM, Chiu HH, Liao ST, Chen YT, Chang HC, Wen KC - Evid Based Complement Alternat Med (2013)

Effect of FME on the UV-induced (a) type I procollagen expression in human fibroblasts; (b) MMP-1, MMP-3, and MMP-9 expression in human fibroblasts; (c) MMP-9 by gelatin zymography in the culture medium of human fibroblasts; and (d) MAP kinases expression in human fibroblasts.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3713360&req=5

fig6: Effect of FME on the UV-induced (a) type I procollagen expression in human fibroblasts; (b) MMP-1, MMP-3, and MMP-9 expression in human fibroblasts; (c) MMP-9 by gelatin zymography in the culture medium of human fibroblasts; and (d) MAP kinases expression in human fibroblasts.
Mentions: Fibroblasts were treated with FME (5–50 μg/mL) for 24 h after exposure to UVB (80 mJ/cm2) and the levels of type I procollagen were measured using Western blot. EGCG was used as the positive control. FME treatment (≥10 μg/mL) resulted in a significant increase in the production of type I procollagen relative to that of control cells (Figure 6(a)).

Bottom Line: The IC50 values were 2.1  μ g/mL for DPPH radical scavenging ability, 366.8  μ g/mL for superoxide anion scavenging ability, 178.9  μ g/mL for hydrogen peroxide scavenging ability, and 230.9  μ g/mL for hydroxyl radical scavenging ability.In human fibroblasts, FME at 10  μ g/mL was shown to be a potent scavenger of UV-induced reactive oxygen species (ROS).The antioxidant and anti-photoaging properties of FME make it an ideal anti-intrinsic aging and anti-photoaging agent.

View Article: PubMed Central - PubMed

Affiliation: Department of Cosmeceutics, China Medical University, Taichung 404, Taiwan.

ABSTRACT
In this study, we investigated the antioxidant activity and anti-photoaging properties of an extract of Flemingia macrophylla, a plant rich in isoflavonoid content. Pretreatment of fibroblasts with Flemingia macrophylla extract (FME) inhibited elastase activity, promoted the protein expression of type I procollagen, and attenuated the phosphorylation of mitogen-activated protein (MAP) kinase and the protein expression of matrix-metalloproteinase- (MMP-) 1, 3, and 9. The IC50 values were 2.1  μ g/mL for DPPH radical scavenging ability, 366.8  μ g/mL for superoxide anion scavenging ability, 178.9  μ g/mL for hydrogen peroxide scavenging ability, and 230.9  μ g/mL for hydroxyl radical scavenging ability. Also, exposure of erythrocytes to various concentrations of FME (50-500  μ g/mL) resulted in a dose- and time-dependent inhibition of AAPH-induced hemolysis. In human fibroblasts, FME at 10  μ g/mL was shown to be a potent scavenger of UV-induced reactive oxygen species (ROS). The antioxidant and anti-photoaging properties of FME make it an ideal anti-intrinsic aging and anti-photoaging agent.

No MeSH data available.


Related in: MedlinePlus