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Mutations in HISTONE ACETYLTRANSFERASE1 affect sugar response and gene expression in Arabidopsis.

Heisel TJ, Li CY, Grey KM, Gibson SI - Front Plant Sci (2013)

Bottom Line: Histone acetyltransferases increase transcription of specific genes by acetylating histones associated with those genes.SnRK1 complexes have been shown to function as central regulators of plant nutrient and energy status.Involvement of a histone acetyltransferase in sugar response provides a possible mechanism whereby nutritional status could exert long-term effects on plant development and metabolism.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Biology, Microbial and Plant Genomics Institute, University of Minnesota Saint Paul, MN, USA.

ABSTRACT
Nutrient response networks are likely to have been among the first response networks to evolve, as the ability to sense and respond to the levels of available nutrients is critical for all organisms. Although several forward genetic screens have been successful in identifying components of plant sugar-response networks, many components remain to be identified. Toward this end, a reverse genetic screen was conducted in Arabidopsis thaliana to identify additional components of sugar-response networks. This screen was based on the rationale that some of the genes involved in sugar-response networks are likely to be themselves sugar regulated at the steady-state mRNA level and to encode proteins with activities commonly associated with response networks. This rationale was validated by the identification of hac1 mutants that are defective in sugar response. HAC1 encodes a histone acetyltransferase. Histone acetyltransferases increase transcription of specific genes by acetylating histones associated with those genes. Mutations in HAC1 also cause reduced fertility, a moderate degree of resistance to paclobutrazol and altered transcript levels of specific genes. Previous research has shown that hac1 mutants exhibit delayed flowering. The sugar-response and fertility defects of hac1 mutants may be partially explained by decreased expression of AtPV42a and AtPV42b, which are putative components of plant SnRK1 complexes. SnRK1 complexes have been shown to function as central regulators of plant nutrient and energy status. Involvement of a histone acetyltransferase in sugar response provides a possible mechanism whereby nutritional status could exert long-term effects on plant development and metabolism.

No MeSH data available.


Related in: MedlinePlus

Mutations in HAC1 reduce seed production. Mutant and wild-type plants were grown to maturity. The numbers of siliques on each plant were counted. Siliques were then collected from each plant and the total number of seeds produced per silique and per plant determined. Error bars indicate standard deviations. Mutant and wild-type results differed with: *p < 0.1; **p < 0.05; or ***p < 0.01, according to a Student's t-test. N = 2 (hac1-3, wild-type, WT) or 3 (hac1-2, hac1-6).
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Figure 5: Mutations in HAC1 reduce seed production. Mutant and wild-type plants were grown to maturity. The numbers of siliques on each plant were counted. Siliques were then collected from each plant and the total number of seeds produced per silique and per plant determined. Error bars indicate standard deviations. Mutant and wild-type results differed with: *p < 0.1; **p < 0.05; or ***p < 0.01, according to a Student's t-test. N = 2 (hac1-3, wild-type, WT) or 3 (hac1-2, hac1-6).

Mentions: Adult hac1 plants were observed to produce many siliques that were extremely stunted and which contained few or no seeds. To characterize this phenotype the numbers of siliques produced per plant, seeds produced per plant, and seeds produced per silique were determined for hac1 and wild-type plants. Although the average number of siliques produced by hac1 plants was greater than the average number produced by wild-type plants, these differences in silique number were not statistically significant between the samples analyzed (Figure 5). However, analysis of the number of seeds produced per plant revealed that hac1 plants produce significantly fewer seeds than do wild-type plants (Figure 5). As the reduced seed production of hac1 plants does not appear to be due to a reduction in the number of siliques per plant, the numbers of seeds per silique were analyzed. Siliques produced by hac1 plants were found to contain significantly fewer seeds on average than siliques produced by wild-type plants (Figure 5). Thus, the reduced seed production of hac1 plants appears to be due to a decrease in the number of seeds per silique, rather than in the number of siliques per plant.


Mutations in HISTONE ACETYLTRANSFERASE1 affect sugar response and gene expression in Arabidopsis.

Heisel TJ, Li CY, Grey KM, Gibson SI - Front Plant Sci (2013)

Mutations in HAC1 reduce seed production. Mutant and wild-type plants were grown to maturity. The numbers of siliques on each plant were counted. Siliques were then collected from each plant and the total number of seeds produced per silique and per plant determined. Error bars indicate standard deviations. Mutant and wild-type results differed with: *p < 0.1; **p < 0.05; or ***p < 0.01, according to a Student's t-test. N = 2 (hac1-3, wild-type, WT) or 3 (hac1-2, hac1-6).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3713338&req=5

Figure 5: Mutations in HAC1 reduce seed production. Mutant and wild-type plants were grown to maturity. The numbers of siliques on each plant were counted. Siliques were then collected from each plant and the total number of seeds produced per silique and per plant determined. Error bars indicate standard deviations. Mutant and wild-type results differed with: *p < 0.1; **p < 0.05; or ***p < 0.01, according to a Student's t-test. N = 2 (hac1-3, wild-type, WT) or 3 (hac1-2, hac1-6).
Mentions: Adult hac1 plants were observed to produce many siliques that were extremely stunted and which contained few or no seeds. To characterize this phenotype the numbers of siliques produced per plant, seeds produced per plant, and seeds produced per silique were determined for hac1 and wild-type plants. Although the average number of siliques produced by hac1 plants was greater than the average number produced by wild-type plants, these differences in silique number were not statistically significant between the samples analyzed (Figure 5). However, analysis of the number of seeds produced per plant revealed that hac1 plants produce significantly fewer seeds than do wild-type plants (Figure 5). As the reduced seed production of hac1 plants does not appear to be due to a reduction in the number of siliques per plant, the numbers of seeds per silique were analyzed. Siliques produced by hac1 plants were found to contain significantly fewer seeds on average than siliques produced by wild-type plants (Figure 5). Thus, the reduced seed production of hac1 plants appears to be due to a decrease in the number of seeds per silique, rather than in the number of siliques per plant.

Bottom Line: Histone acetyltransferases increase transcription of specific genes by acetylating histones associated with those genes.SnRK1 complexes have been shown to function as central regulators of plant nutrient and energy status.Involvement of a histone acetyltransferase in sugar response provides a possible mechanism whereby nutritional status could exert long-term effects on plant development and metabolism.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Biology, Microbial and Plant Genomics Institute, University of Minnesota Saint Paul, MN, USA.

ABSTRACT
Nutrient response networks are likely to have been among the first response networks to evolve, as the ability to sense and respond to the levels of available nutrients is critical for all organisms. Although several forward genetic screens have been successful in identifying components of plant sugar-response networks, many components remain to be identified. Toward this end, a reverse genetic screen was conducted in Arabidopsis thaliana to identify additional components of sugar-response networks. This screen was based on the rationale that some of the genes involved in sugar-response networks are likely to be themselves sugar regulated at the steady-state mRNA level and to encode proteins with activities commonly associated with response networks. This rationale was validated by the identification of hac1 mutants that are defective in sugar response. HAC1 encodes a histone acetyltransferase. Histone acetyltransferases increase transcription of specific genes by acetylating histones associated with those genes. Mutations in HAC1 also cause reduced fertility, a moderate degree of resistance to paclobutrazol and altered transcript levels of specific genes. Previous research has shown that hac1 mutants exhibit delayed flowering. The sugar-response and fertility defects of hac1 mutants may be partially explained by decreased expression of AtPV42a and AtPV42b, which are putative components of plant SnRK1 complexes. SnRK1 complexes have been shown to function as central regulators of plant nutrient and energy status. Involvement of a histone acetyltransferase in sugar response provides a possible mechanism whereby nutritional status could exert long-term effects on plant development and metabolism.

No MeSH data available.


Related in: MedlinePlus