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The effect of continuous epidural electrical stimulation on neuronal proliferation in cerebral ischemic rats.

Kang C, Yang CY, Kim JH, Moon SK, Lee S, Park SA, Han EH, Zhang LQ - Ann Rehabil Med (2013)

Bottom Line: On the SPRT, the motor function in paralytic forelimbs of the ES group was significantly improved.In the ES group, NeuN+ cells in the ischemic hemisphere and DCX+ cells and BrdU+ cells in the opposite hemisphere tended to increase compared to those in the contralateral.The continuous epidural ES of the ischemic sensorimotor cortex induced a significant improvement in the motor function and tended to increase neural cell proliferation in the ischemic hemisphere and the neural regeneration in the opposite hemisphere.

View Article: PubMed Central - PubMed

Affiliation: Department of Physical Medicine and Rehabilitation & Regional Cerebro-Cardiovascular Center, Institute of Wonkwang Medical Science, Wonkwang University School of Medicine, Iksan, Korea.

ABSTRACT

Objective: To investigate the effect of electrical stimulation (ES) on the recovery of motor skill and neuronal cell proliferation.

Methods: The male Sprague-Dawley rats were implanted with an epidural electrode over the peri-ischemic area after photothrombotic stroke in the dominant sensorimotor cortex. All rats were randomly assigned into the ES group and control group. The behavioral test of a single pellet reaching task (SPRT) and neurological examinations including the Schabitz's photothrombotic neurological score and the Menzies test were conducted for 2 weeks. After 14 days, coronal sections were obtained and immunostained for neuronal cell differentiation markers including bromodeoxyuridine (BrdU), neuron-specific nuclear protein (NeuN), and doublecortin (DCX).

Results: On the SPRT, the motor function in paralytic forelimbs of the ES group was significantly improved. There were no significant differences in neurological examinations and neuronal cell differentiation markers except for the significantly increased number of DCX+ cells in the corpus callosum of the ES group (p<0.05). But in the ES group, the number of NeuN+ cells in the ischemic cortex and the number of NeuN+ cells and DCX+ cells in the ischemic striatum tended to increase. In the ES group, NeuN+ cells in the ischemic hemisphere and DCX+ cells and BrdU+ cells in the opposite hemisphere tended to increase compared to those in the contralateral.

Conclusion: The continuous epidural ES of the ischemic sensorimotor cortex induced a significant improvement in the motor function and tended to increase neural cell proliferation in the ischemic hemisphere and the neural regeneration in the opposite hemisphere.

No MeSH data available.


Related in: MedlinePlus

The neurological examinations including Schabitz's photothrombotic neurological score and Menzies test were performed for neurological evaluation of behavioral recovery. There were significant improvements in each neurological test for 2 weeks after photothrombotic stroke (p<0.05), but not between two groups at each test. ES, electrical stimulation; Cont, the operation control group.
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Figure 3: The neurological examinations including Schabitz's photothrombotic neurological score and Menzies test were performed for neurological evaluation of behavioral recovery. There were significant improvements in each neurological test for 2 weeks after photothrombotic stroke (p<0.05), but not between two groups at each test. ES, electrical stimulation; Cont, the operation control group.

Mentions: Both the Schabitz test and Menzies test demonstrated a significant recovery from neurologic damage in the two groups with the passage of time after the surgery for induction of cerebral ischemia (Schabitz test: F=51.780, p<0.001; Menzies test: F=19.819, p<0.001) (Fig. 3). More specifically, Schabitz test demonstrated a significantly increased neurologic recovery on the 5th, 8th, 11th, and 13th day compared to that on the 2nd day after induction of cerebral ischemia, whereas Menzies test demonstrated a significantly increased neurologic recovery on the 11th and 13th day compared to that on the 2nd day after induction of cerebral ischemia (Bonferroni p<0.05 in both groups). These two neurologic tests did not show any significant difference between the two groups (Schabitz test: F=0.283, p=0.602; Menzies test: F=0.076, p=0.787) (Fig. 3).


The effect of continuous epidural electrical stimulation on neuronal proliferation in cerebral ischemic rats.

Kang C, Yang CY, Kim JH, Moon SK, Lee S, Park SA, Han EH, Zhang LQ - Ann Rehabil Med (2013)

The neurological examinations including Schabitz's photothrombotic neurological score and Menzies test were performed for neurological evaluation of behavioral recovery. There were significant improvements in each neurological test for 2 weeks after photothrombotic stroke (p<0.05), but not between two groups at each test. ES, electrical stimulation; Cont, the operation control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3713286&req=5

Figure 3: The neurological examinations including Schabitz's photothrombotic neurological score and Menzies test were performed for neurological evaluation of behavioral recovery. There were significant improvements in each neurological test for 2 weeks after photothrombotic stroke (p<0.05), but not between two groups at each test. ES, electrical stimulation; Cont, the operation control group.
Mentions: Both the Schabitz test and Menzies test demonstrated a significant recovery from neurologic damage in the two groups with the passage of time after the surgery for induction of cerebral ischemia (Schabitz test: F=51.780, p<0.001; Menzies test: F=19.819, p<0.001) (Fig. 3). More specifically, Schabitz test demonstrated a significantly increased neurologic recovery on the 5th, 8th, 11th, and 13th day compared to that on the 2nd day after induction of cerebral ischemia, whereas Menzies test demonstrated a significantly increased neurologic recovery on the 11th and 13th day compared to that on the 2nd day after induction of cerebral ischemia (Bonferroni p<0.05 in both groups). These two neurologic tests did not show any significant difference between the two groups (Schabitz test: F=0.283, p=0.602; Menzies test: F=0.076, p=0.787) (Fig. 3).

Bottom Line: On the SPRT, the motor function in paralytic forelimbs of the ES group was significantly improved.In the ES group, NeuN+ cells in the ischemic hemisphere and DCX+ cells and BrdU+ cells in the opposite hemisphere tended to increase compared to those in the contralateral.The continuous epidural ES of the ischemic sensorimotor cortex induced a significant improvement in the motor function and tended to increase neural cell proliferation in the ischemic hemisphere and the neural regeneration in the opposite hemisphere.

View Article: PubMed Central - PubMed

Affiliation: Department of Physical Medicine and Rehabilitation & Regional Cerebro-Cardiovascular Center, Institute of Wonkwang Medical Science, Wonkwang University School of Medicine, Iksan, Korea.

ABSTRACT

Objective: To investigate the effect of electrical stimulation (ES) on the recovery of motor skill and neuronal cell proliferation.

Methods: The male Sprague-Dawley rats were implanted with an epidural electrode over the peri-ischemic area after photothrombotic stroke in the dominant sensorimotor cortex. All rats were randomly assigned into the ES group and control group. The behavioral test of a single pellet reaching task (SPRT) and neurological examinations including the Schabitz's photothrombotic neurological score and the Menzies test were conducted for 2 weeks. After 14 days, coronal sections were obtained and immunostained for neuronal cell differentiation markers including bromodeoxyuridine (BrdU), neuron-specific nuclear protein (NeuN), and doublecortin (DCX).

Results: On the SPRT, the motor function in paralytic forelimbs of the ES group was significantly improved. There were no significant differences in neurological examinations and neuronal cell differentiation markers except for the significantly increased number of DCX+ cells in the corpus callosum of the ES group (p<0.05). But in the ES group, the number of NeuN+ cells in the ischemic cortex and the number of NeuN+ cells and DCX+ cells in the ischemic striatum tended to increase. In the ES group, NeuN+ cells in the ischemic hemisphere and DCX+ cells and BrdU+ cells in the opposite hemisphere tended to increase compared to those in the contralateral.

Conclusion: The continuous epidural ES of the ischemic sensorimotor cortex induced a significant improvement in the motor function and tended to increase neural cell proliferation in the ischemic hemisphere and the neural regeneration in the opposite hemisphere.

No MeSH data available.


Related in: MedlinePlus