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Expression of surface markers and myogenic potential of rat bone marrow- and adipose-derived stem cells: a comparative study.

Bayati V, Hashemitabar M, Gazor R, Nejatbakhsh R, Bijannejad D - Anat Cell Biol (2013)

Bottom Line: The expression of CD44, CD45, CD73, and CD90 on bone marrow- and adipose-derived MSCs was characterized using flow cytometry.Our results reveal that the pattern of CD marker expression differs between these 2 types of MSCs to some extent, whereas no significant difference was observed with respect to their myogenic differentiation potential.Therefore, we concluded that despite the differences observed in the biological features of these 2 types of MSCs, their myogenic potential appears to be similar, and that adipose-derived stem cells may be useful in skeletal muscle tissue engineering, due to their easy isolation and capacity for rapid expansion in a short time span.

View Article: PubMed Central - PubMed

Affiliation: Cellular and Molecular Research Center, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. ; Department of Anatomical Science, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

ABSTRACT
In recent years, examination and comparison of the biological characteristics of bone marrow- and adipose-derived mesenchymal stem cells (MSCs) from various perspectives have come into the focus of stem cell research, as these cells should be well characterized in order to utilize them in future cellular therapies. Therefore, in the present study, surface protein markers and the skeletal myogenic differentiation potential of rat bone marrow- and adipose-derived MSCs were examined. The expression of CD44, CD45, CD73, and CD90 on bone marrow- and adipose-derived MSCs was characterized using flow cytometry. Subsequently, the stem cells were differentiated into myogenic lineages, and the expression of the skeletal myogenic markers MyoD1, Myog, and Myh2 was studied in cells using real time polymerase chain reaction and immunofluorescence. Our results reveal that the pattern of CD marker expression differs between these 2 types of MSCs to some extent, whereas no significant difference was observed with respect to their myogenic differentiation potential. Therefore, we concluded that despite the differences observed in the biological features of these 2 types of MSCs, their myogenic potential appears to be similar, and that adipose-derived stem cells may be useful in skeletal muscle tissue engineering, due to their easy isolation and capacity for rapid expansion in a short time span.

No MeSH data available.


Related in: MedlinePlus

Expression and the intracellular position of myogenic markers MyoD1, Myog, and myosin (fast skeletal) in bone marrow-derived mesenchymal stem cells (BMSCs) and adipose-derived stem cells (ASCs) in the absence or presence of chemical growth factors. MyoD1 (A) and Myog (B), as skeletal muscle transcription factors, were mostly concentrated in the nuclei (arrows). (C) Myosin (fast skeletal). Undifferentiated stem cells were taken as a negative control, and the cell line L6 was considered as positive control (A-C, ×630).
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Figure 5: Expression and the intracellular position of myogenic markers MyoD1, Myog, and myosin (fast skeletal) in bone marrow-derived mesenchymal stem cells (BMSCs) and adipose-derived stem cells (ASCs) in the absence or presence of chemical growth factors. MyoD1 (A) and Myog (B), as skeletal muscle transcription factors, were mostly concentrated in the nuclei (arrows). (C) Myosin (fast skeletal). Undifferentiated stem cells were taken as a negative control, and the cell line L6 was considered as positive control (A-C, ×630).

Mentions: Myogenic differentiation analysis by immunofluorescence demonstrated that neither BMSCs nor ASCs in the control groups were positive for MyoD1, Myog, or fast skeletal myosin. Additionally, there was no substantial qualitative difference between myogenically differentiated BMSCs and ASCs when they were examined by fluorescence microscopy, or when their images were further examined using Adobe Photoshop software (Fig. 5A-C). The expression levels of MyoD1, Myog, and Myh2 mRNA in BMSCs and ASCs were examined by RT-PCR (Fig. 6). The expression of Myog and Myh2 did not differ significantly between the BMSCs and ASCs. However, MyoD1 expression tended to be significantly higher in the BMSCs than in the ASCs (P<0.05).


Expression of surface markers and myogenic potential of rat bone marrow- and adipose-derived stem cells: a comparative study.

Bayati V, Hashemitabar M, Gazor R, Nejatbakhsh R, Bijannejad D - Anat Cell Biol (2013)

Expression and the intracellular position of myogenic markers MyoD1, Myog, and myosin (fast skeletal) in bone marrow-derived mesenchymal stem cells (BMSCs) and adipose-derived stem cells (ASCs) in the absence or presence of chemical growth factors. MyoD1 (A) and Myog (B), as skeletal muscle transcription factors, were mostly concentrated in the nuclei (arrows). (C) Myosin (fast skeletal). Undifferentiated stem cells were taken as a negative control, and the cell line L6 was considered as positive control (A-C, ×630).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3713275&req=5

Figure 5: Expression and the intracellular position of myogenic markers MyoD1, Myog, and myosin (fast skeletal) in bone marrow-derived mesenchymal stem cells (BMSCs) and adipose-derived stem cells (ASCs) in the absence or presence of chemical growth factors. MyoD1 (A) and Myog (B), as skeletal muscle transcription factors, were mostly concentrated in the nuclei (arrows). (C) Myosin (fast skeletal). Undifferentiated stem cells were taken as a negative control, and the cell line L6 was considered as positive control (A-C, ×630).
Mentions: Myogenic differentiation analysis by immunofluorescence demonstrated that neither BMSCs nor ASCs in the control groups were positive for MyoD1, Myog, or fast skeletal myosin. Additionally, there was no substantial qualitative difference between myogenically differentiated BMSCs and ASCs when they were examined by fluorescence microscopy, or when their images were further examined using Adobe Photoshop software (Fig. 5A-C). The expression levels of MyoD1, Myog, and Myh2 mRNA in BMSCs and ASCs were examined by RT-PCR (Fig. 6). The expression of Myog and Myh2 did not differ significantly between the BMSCs and ASCs. However, MyoD1 expression tended to be significantly higher in the BMSCs than in the ASCs (P<0.05).

Bottom Line: The expression of CD44, CD45, CD73, and CD90 on bone marrow- and adipose-derived MSCs was characterized using flow cytometry.Our results reveal that the pattern of CD marker expression differs between these 2 types of MSCs to some extent, whereas no significant difference was observed with respect to their myogenic differentiation potential.Therefore, we concluded that despite the differences observed in the biological features of these 2 types of MSCs, their myogenic potential appears to be similar, and that adipose-derived stem cells may be useful in skeletal muscle tissue engineering, due to their easy isolation and capacity for rapid expansion in a short time span.

View Article: PubMed Central - PubMed

Affiliation: Cellular and Molecular Research Center, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. ; Department of Anatomical Science, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

ABSTRACT
In recent years, examination and comparison of the biological characteristics of bone marrow- and adipose-derived mesenchymal stem cells (MSCs) from various perspectives have come into the focus of stem cell research, as these cells should be well characterized in order to utilize them in future cellular therapies. Therefore, in the present study, surface protein markers and the skeletal myogenic differentiation potential of rat bone marrow- and adipose-derived MSCs were examined. The expression of CD44, CD45, CD73, and CD90 on bone marrow- and adipose-derived MSCs was characterized using flow cytometry. Subsequently, the stem cells were differentiated into myogenic lineages, and the expression of the skeletal myogenic markers MyoD1, Myog, and Myh2 was studied in cells using real time polymerase chain reaction and immunofluorescence. Our results reveal that the pattern of CD marker expression differs between these 2 types of MSCs to some extent, whereas no significant difference was observed with respect to their myogenic differentiation potential. Therefore, we concluded that despite the differences observed in the biological features of these 2 types of MSCs, their myogenic potential appears to be similar, and that adipose-derived stem cells may be useful in skeletal muscle tissue engineering, due to their easy isolation and capacity for rapid expansion in a short time span.

No MeSH data available.


Related in: MedlinePlus