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Heterogeneity of glandular cells in the human salivary glands: an immunohistochemical study using elderly adult and fetal specimens.

Katori Y, Hayashi S, Takanashi Y, Kim JH, Abe S, Murakami G, Kawase T - Anat Cell Biol (2013)

Bottom Line: In both adults and fetuses, a mosaic expression pattern was usually evident for each of the examined proteins.A difference in immunoreactivity for the nerve markers GFAP and S100 was observed between the major and minor glands.A mosaic expression pattern suggested that the immunoreactivity against nerve protein markers in myoepithelial cells could not be due to the persistence of neural crest remnants or the physiological status of the gland, such as age-related degeneration.

View Article: PubMed Central - PubMed

Affiliation: Division of Otorhinolaryngology, Sendai Municipal Hospital, Sendai, Japan.

ABSTRACT
Using immunohistochemical staining for alpha-smooth muscle actin (α-SMA), glial fibrillary acidic protein (GFAP), S100 protein (S100), p63, cytokeratin 14 (CK14), and cytokeratin 19 (CK19), we studied acinar and myoepithelial cells of major and minor salivary glands obtained from 14 donated cadavers (78-92 years old) and 5 donated fetuses (aborted at 15-16 weeks of gestation). CK and p63 expression was investigated only in the adult specimens. SMA was detected in all adult glands as well as in fetal sublingual and pharyngeal glands. GFAP expression was seen in a limited number of cells in adult glands, but was highly expressed in fetal pharyngeal glands. S100-positive myoepithelial-like cells were present in adult minor glands as well as in fetal sublingual and pharyngeal glands. Expression of p63 was evident in the ducts of adult glands. CK14 immunoreactivity was observed in a limited number of glandular cells in adults, in contrast to consistent expression of CK19. In both adults and fetuses, a mosaic expression pattern was usually evident for each of the examined proteins. A difference in immunoreactivity for the nerve markers GFAP and S100 was observed between the major and minor glands. Thus, in the present histologic study, we distinguished between the specific gland types on the basis of their immunohistochemical staining. A mosaic expression pattern suggested that the immunoreactivity against nerve protein markers in myoepithelial cells could not be due to the persistence of neural crest remnants or the physiological status of the gland, such as age-related degeneration.

No MeSH data available.


Related in: MedlinePlus

Immunohistochemistry of a small palatal gland at the soft palate (88-year-old man). (A) p63, (B) alpha-smooth muscle actin (α-SMA), (C) glial fibrillary acidic protein (GFAP), (D) S100 protein (S100), (E) cytokeratin 19 (CK19), (F) cytokeratin 14 (CK14). (A) p63 is weakly expressed in some thick ducts (arrows). (B) SMA is expressed in a limited number of myoepithelial-like cells (arrows). (C) GFAP is weakly expressed in some ducts (arrows). (D) S100 expression is seen in myoepithelial-like cells (arrows) as well as in nerves. (E) CK19 is expressed in all the ducts. (F) CK14 reactivity is seen in the debris in the thick ducts (stars) as well as in some myoepithelial-like cells (arrows). All micrographs were taken at the same magnification. Scale bar in (A)=0.1 mm (A-F).
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Figure 5: Immunohistochemistry of a small palatal gland at the soft palate (88-year-old man). (A) p63, (B) alpha-smooth muscle actin (α-SMA), (C) glial fibrillary acidic protein (GFAP), (D) S100 protein (S100), (E) cytokeratin 19 (CK19), (F) cytokeratin 14 (CK14). (A) p63 is weakly expressed in some thick ducts (arrows). (B) SMA is expressed in a limited number of myoepithelial-like cells (arrows). (C) GFAP is weakly expressed in some ducts (arrows). (D) S100 expression is seen in myoepithelial-like cells (arrows) as well as in nerves. (E) CK19 is expressed in all the ducts. (F) CK14 reactivity is seen in the debris in the thick ducts (stars) as well as in some myoepithelial-like cells (arrows). All micrographs were taken at the same magnification. Scale bar in (A)=0.1 mm (A-F).

Mentions: Among the minor glands, the small lingual gland in the dorsal surface of the tongue (Fig. 3) was characterized by strong expression of S100 in most of the myoepithelial-like cells; however, α-SMA and GFAP both showed a mosaic pattern of expression. In contrast, the small pharyngeal gland near the tonsillar fossa (Fig. 4) displayed a mosaic pattern of S100 immunoexpression, whereas α-SMA was strongly expressed in all myoepithelial-like cells. Unlike the lingual and pharyngeal glands, the small palatal gland in the soft palate showed individual differences in the numbers of GFAP- and α-SMA-positive myoepithelial-like cells (Figs. 5, 6), constituting less than 50% of the cells in the acinus of 10 specimens (Fig. 5B, C) in contrast to almost 100% in the acinus of the 4 remaining specimens (Fig. 6A, B). In the palatal gland, S100 expression was observed in some acinus-like and most of the myoepithelial-like cells. Expression patterns for CK19, CK14, and p63 were similar in the minor and major salivary glands. The results of the immunohistochemical analysis of the adult specimens are summarized in Table 1.


Heterogeneity of glandular cells in the human salivary glands: an immunohistochemical study using elderly adult and fetal specimens.

Katori Y, Hayashi S, Takanashi Y, Kim JH, Abe S, Murakami G, Kawase T - Anat Cell Biol (2013)

Immunohistochemistry of a small palatal gland at the soft palate (88-year-old man). (A) p63, (B) alpha-smooth muscle actin (α-SMA), (C) glial fibrillary acidic protein (GFAP), (D) S100 protein (S100), (E) cytokeratin 19 (CK19), (F) cytokeratin 14 (CK14). (A) p63 is weakly expressed in some thick ducts (arrows). (B) SMA is expressed in a limited number of myoepithelial-like cells (arrows). (C) GFAP is weakly expressed in some ducts (arrows). (D) S100 expression is seen in myoepithelial-like cells (arrows) as well as in nerves. (E) CK19 is expressed in all the ducts. (F) CK14 reactivity is seen in the debris in the thick ducts (stars) as well as in some myoepithelial-like cells (arrows). All micrographs were taken at the same magnification. Scale bar in (A)=0.1 mm (A-F).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3713274&req=5

Figure 5: Immunohistochemistry of a small palatal gland at the soft palate (88-year-old man). (A) p63, (B) alpha-smooth muscle actin (α-SMA), (C) glial fibrillary acidic protein (GFAP), (D) S100 protein (S100), (E) cytokeratin 19 (CK19), (F) cytokeratin 14 (CK14). (A) p63 is weakly expressed in some thick ducts (arrows). (B) SMA is expressed in a limited number of myoepithelial-like cells (arrows). (C) GFAP is weakly expressed in some ducts (arrows). (D) S100 expression is seen in myoepithelial-like cells (arrows) as well as in nerves. (E) CK19 is expressed in all the ducts. (F) CK14 reactivity is seen in the debris in the thick ducts (stars) as well as in some myoepithelial-like cells (arrows). All micrographs were taken at the same magnification. Scale bar in (A)=0.1 mm (A-F).
Mentions: Among the minor glands, the small lingual gland in the dorsal surface of the tongue (Fig. 3) was characterized by strong expression of S100 in most of the myoepithelial-like cells; however, α-SMA and GFAP both showed a mosaic pattern of expression. In contrast, the small pharyngeal gland near the tonsillar fossa (Fig. 4) displayed a mosaic pattern of S100 immunoexpression, whereas α-SMA was strongly expressed in all myoepithelial-like cells. Unlike the lingual and pharyngeal glands, the small palatal gland in the soft palate showed individual differences in the numbers of GFAP- and α-SMA-positive myoepithelial-like cells (Figs. 5, 6), constituting less than 50% of the cells in the acinus of 10 specimens (Fig. 5B, C) in contrast to almost 100% in the acinus of the 4 remaining specimens (Fig. 6A, B). In the palatal gland, S100 expression was observed in some acinus-like and most of the myoepithelial-like cells. Expression patterns for CK19, CK14, and p63 were similar in the minor and major salivary glands. The results of the immunohistochemical analysis of the adult specimens are summarized in Table 1.

Bottom Line: In both adults and fetuses, a mosaic expression pattern was usually evident for each of the examined proteins.A difference in immunoreactivity for the nerve markers GFAP and S100 was observed between the major and minor glands.A mosaic expression pattern suggested that the immunoreactivity against nerve protein markers in myoepithelial cells could not be due to the persistence of neural crest remnants or the physiological status of the gland, such as age-related degeneration.

View Article: PubMed Central - PubMed

Affiliation: Division of Otorhinolaryngology, Sendai Municipal Hospital, Sendai, Japan.

ABSTRACT
Using immunohistochemical staining for alpha-smooth muscle actin (α-SMA), glial fibrillary acidic protein (GFAP), S100 protein (S100), p63, cytokeratin 14 (CK14), and cytokeratin 19 (CK19), we studied acinar and myoepithelial cells of major and minor salivary glands obtained from 14 donated cadavers (78-92 years old) and 5 donated fetuses (aborted at 15-16 weeks of gestation). CK and p63 expression was investigated only in the adult specimens. SMA was detected in all adult glands as well as in fetal sublingual and pharyngeal glands. GFAP expression was seen in a limited number of cells in adult glands, but was highly expressed in fetal pharyngeal glands. S100-positive myoepithelial-like cells were present in adult minor glands as well as in fetal sublingual and pharyngeal glands. Expression of p63 was evident in the ducts of adult glands. CK14 immunoreactivity was observed in a limited number of glandular cells in adults, in contrast to consistent expression of CK19. In both adults and fetuses, a mosaic expression pattern was usually evident for each of the examined proteins. A difference in immunoreactivity for the nerve markers GFAP and S100 was observed between the major and minor glands. Thus, in the present histologic study, we distinguished between the specific gland types on the basis of their immunohistochemical staining. A mosaic expression pattern suggested that the immunoreactivity against nerve protein markers in myoepithelial cells could not be due to the persistence of neural crest remnants or the physiological status of the gland, such as age-related degeneration.

No MeSH data available.


Related in: MedlinePlus