Limits...
Development of somatic hybrids Solanum × michoacanum Bitter. (Rydb.) (+) S. tuberosum L. and autofused 4x S. × michoacanum plants as potential sources of late blight resistance for potato breeding.

Smyda P, Jakuczun H, Dębski K, Sliwka J, Thieme R, Nachtigall M, Wasilewicz-Flis I, Zimnoch-Guzowska E - Plant Cell Rep. (2013)

Bottom Line: Solanum × michoacanum (Bitter.) Rydb. (mch) is a wild diploid (2n = 2x = 24) potato species derived from spontaneous cross of S. bulbocastanum and S. pinnatisectum.In total 18,775 calli were obtained from postfusion products from which 1,482 formed shoots.Resistant forms have adequate pollen stainability for use in crossing programme and are a promising material useful for introgression resistance from mch into the cultivated potato background.

View Article: PubMed Central - PubMed

Affiliation: Plant Breeding and Acclimatization Institute-National Research Institute (IHAR-PIB), Młochów Research Centre, Platanowa 19, 05831, Młochów, Poland. p.smyda@ihar.edu.pl

ABSTRACT

Key message: Phytophthora infestans resistant somatic hybrids of S. × michoacanum (+) S. tuberosum and autofused 4 x S. × michoacanum were obtained. Our material is promising to introgress resistance from S. × michoacanum into cultivated potato background. Solanum × michoacanum (Bitter.) Rydb. (mch) is a wild diploid (2n = 2x = 24) potato species derived from spontaneous cross of S. bulbocastanum and S. pinnatisectum. This hybrid is a 1 EBN (endosperm balance number) species and can cross effectively only with other 1 EBN species. Plants of mch are resistant to Phytophthora infestans (Mont) de Bary. To introgress late blight resistance genes from mch into S. tuberosum (tbr), genepool somatic hybridization between mch and susceptible diploid potato clones (2n = 2x = 24) or potato cultivar Rywal (2n = 4x = 48) was performed. In total 18,775 calli were obtained from postfusion products from which 1,482 formed shoots. The Simple Sequence Repeat (SSR), Cleaved Amplified Polymorphic Sequences (CAPS) and Random Amplified Polymorphic DNA (RAPD) analyses confirmed hybrid nature of 228 plants and 116 autofused 4x mch. After evaluation of morphological features, flowering, pollen stainability, tuberization and ploidy level, 118 somatic hybrids and 116 autofused 4x mch were tested for late blight resistance using the detached leaf assay. After two seasons of testing three somatic hybrids and 109 4x mch were resistant. Resistant forms have adequate pollen stainability for use in crossing programme and are a promising material useful for introgression resistance from mch into the cultivated potato background.

Show MeSH

Related in: MedlinePlus

Exemplary morphology of a tubers and b leaves of somatic hybrid and its parents: mch/39, mch/39 (+) dH Bard and dH Bard (from left to right, respectively)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3713256&req=5

Fig3: Exemplary morphology of a tubers and b leaves of somatic hybrid and its parents: mch/39, mch/39 (+) dH Bard and dH Bard (from left to right, respectively)

Mentions: Morphological observations indicated two distinct groups of plants: one with intermediate phenotype, between parental forms and a second one similar to mch parent. Among plants with intermediate phenotype, some deformations of whole plants, leaves and tubers or various colours of flowers were observed; however, plants and tubers without these deformations were often observed (Fig. 3). Plants similar to mch parents were more luxuriant and had bigger leaves with widen leaflets in comparison to wild forms. Within set of 228 confirmed hybrids, 160 hybrids were tetraploids and 68 were with ploidy level higher than 4x (6x or 8x) (Table 1), what was confirmed by counting of chloroplasts in the guard cells or by flow cytometry. Among 118 tuberizing hybrids, 103 ones were 4x and the ploidy level of 15 forms were more than 4x (they originated from fusion of mch to 4x cv. Rywal). The separate set was represented by 116 autofused 4xmch, which was evidenced by morphological traits and molecular markers. Majority of somatic hybrids and 4xmch flowered intensively. Among them, 85 of 4x somatic hybrids and 113 of autofused 4xmch produced pollen grains which were successfully stained. Stainability of pollen grains of resistant somatic hybrids and 4xmch was between 40–60 and 40–70 %, respectively.Fig. 3


Development of somatic hybrids Solanum × michoacanum Bitter. (Rydb.) (+) S. tuberosum L. and autofused 4x S. × michoacanum plants as potential sources of late blight resistance for potato breeding.

Smyda P, Jakuczun H, Dębski K, Sliwka J, Thieme R, Nachtigall M, Wasilewicz-Flis I, Zimnoch-Guzowska E - Plant Cell Rep. (2013)

Exemplary morphology of a tubers and b leaves of somatic hybrid and its parents: mch/39, mch/39 (+) dH Bard and dH Bard (from left to right, respectively)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3713256&req=5

Fig3: Exemplary morphology of a tubers and b leaves of somatic hybrid and its parents: mch/39, mch/39 (+) dH Bard and dH Bard (from left to right, respectively)
Mentions: Morphological observations indicated two distinct groups of plants: one with intermediate phenotype, between parental forms and a second one similar to mch parent. Among plants with intermediate phenotype, some deformations of whole plants, leaves and tubers or various colours of flowers were observed; however, plants and tubers without these deformations were often observed (Fig. 3). Plants similar to mch parents were more luxuriant and had bigger leaves with widen leaflets in comparison to wild forms. Within set of 228 confirmed hybrids, 160 hybrids were tetraploids and 68 were with ploidy level higher than 4x (6x or 8x) (Table 1), what was confirmed by counting of chloroplasts in the guard cells or by flow cytometry. Among 118 tuberizing hybrids, 103 ones were 4x and the ploidy level of 15 forms were more than 4x (they originated from fusion of mch to 4x cv. Rywal). The separate set was represented by 116 autofused 4xmch, which was evidenced by morphological traits and molecular markers. Majority of somatic hybrids and 4xmch flowered intensively. Among them, 85 of 4x somatic hybrids and 113 of autofused 4xmch produced pollen grains which were successfully stained. Stainability of pollen grains of resistant somatic hybrids and 4xmch was between 40–60 and 40–70 %, respectively.Fig. 3

Bottom Line: Solanum × michoacanum (Bitter.) Rydb. (mch) is a wild diploid (2n = 2x = 24) potato species derived from spontaneous cross of S. bulbocastanum and S. pinnatisectum.In total 18,775 calli were obtained from postfusion products from which 1,482 formed shoots.Resistant forms have adequate pollen stainability for use in crossing programme and are a promising material useful for introgression resistance from mch into the cultivated potato background.

View Article: PubMed Central - PubMed

Affiliation: Plant Breeding and Acclimatization Institute-National Research Institute (IHAR-PIB), Młochów Research Centre, Platanowa 19, 05831, Młochów, Poland. p.smyda@ihar.edu.pl

ABSTRACT

Key message: Phytophthora infestans resistant somatic hybrids of S. × michoacanum (+) S. tuberosum and autofused 4 x S. × michoacanum were obtained. Our material is promising to introgress resistance from S. × michoacanum into cultivated potato background. Solanum × michoacanum (Bitter.) Rydb. (mch) is a wild diploid (2n = 2x = 24) potato species derived from spontaneous cross of S. bulbocastanum and S. pinnatisectum. This hybrid is a 1 EBN (endosperm balance number) species and can cross effectively only with other 1 EBN species. Plants of mch are resistant to Phytophthora infestans (Mont) de Bary. To introgress late blight resistance genes from mch into S. tuberosum (tbr), genepool somatic hybridization between mch and susceptible diploid potato clones (2n = 2x = 24) or potato cultivar Rywal (2n = 4x = 48) was performed. In total 18,775 calli were obtained from postfusion products from which 1,482 formed shoots. The Simple Sequence Repeat (SSR), Cleaved Amplified Polymorphic Sequences (CAPS) and Random Amplified Polymorphic DNA (RAPD) analyses confirmed hybrid nature of 228 plants and 116 autofused 4x mch. After evaluation of morphological features, flowering, pollen stainability, tuberization and ploidy level, 118 somatic hybrids and 116 autofused 4x mch were tested for late blight resistance using the detached leaf assay. After two seasons of testing three somatic hybrids and 109 4x mch were resistant. Resistant forms have adequate pollen stainability for use in crossing programme and are a promising material useful for introgression resistance from mch into the cultivated potato background.

Show MeSH
Related in: MedlinePlus