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Optimization of ultrasonic-assisted extraction and radical-scavenging capacity of phenols and flavonoids from Clerodendrum cyrtophyllum Turcz leaves.

Zhou J, Zheng X, Yang Q, Liang Z, Li D, Yang X, Xu J - PLoS ONE (2013)

Bottom Line: Optimized extraction conditions for UAE from C. cyrtophyllum leaves were as follows: 60.9% ethanol, 85.4 min, and 63.3°C for maximal TPC extraction (16.8 ± 0.2 mg GAE/g DW); 67.7% ethanol, 82.9 min, and 63.0 °C for maximal TFC extraction (49.3 ± 0.4 mg RT/g DW); 48.8% ethanol, 85.1 min, and 63.9 °C for maximal DPPH radical-scavenging capacity (86.8 ± 0.2%); and 50.6% ethanol, 81.3 min, and 63.4 °C for maximal ABTS radical-scavenging capacity (92.9 ± 0.5%).Ethanol concentration was the most important factor in the extraction process.Our work offers optimal extraction conditions for C. cyrtophyllum as a potential source of natural antioxidants.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Protection and Development Utilization of Tropical Crop Germplasm Resources, Ministry of Education, College of Material and Chemical Engineering, Hainan University, Haikou, PR China.

ABSTRACT
Ultrasonic-assisted extraction (UAE) was developed to extract phenolic and flavonoid antioxidants from Clerodendrum cyrtophyllum Turcz leaves. The optimal experimental parameters for antioxidant extraction from C. cyrtophyllum leaves were measured using single-factor experimentation combined with response surface methodology (RSM). Total phenolic content (TPC) and total flavonoid content (TFC) assays were used to quantify antioxidant compounds. Next, antioxidant radical scavenging capacity was measured using 2,2'-diphenyl-1-picrylhydrazyl (DPPH) and 2,2' -azino-bis(3-ethylbenzothiazoline-6-sulphonicacid) (ABTS) radicals. Optimized extraction conditions for UAE from C. cyrtophyllum leaves were as follows: 60.9% ethanol, 85.4 min, and 63.3°C for maximal TPC extraction (16.8 ± 0.2 mg GAE/g DW); 67.7% ethanol, 82.9 min, and 63.0 °C for maximal TFC extraction (49.3 ± 0.4 mg RT/g DW); 48.8% ethanol, 85.1 min, and 63.9 °C for maximal DPPH radical-scavenging capacity (86.8 ± 0.2%); and 50.6% ethanol, 81.3 min, and 63.4 °C for maximal ABTS radical-scavenging capacity (92.9 ± 0.5%). Ethanol concentration was the most important factor in the extraction process. Our work offers optimal extraction conditions for C. cyrtophyllum as a potential source of natural antioxidants.

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Effect of (A) ethanol concentration (extraction time 60 min, extraction temperature 60°C), (B) extraction time (40% ethanol, extraction temperature 60°C), (C) extraction temperature (40% ethanol, extraction time 80 min) on TPC, TFC, DPPH and ABTS radical scavenging capacity from C.cyrtophyllum leaf extracts.
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pone-0068392-g001: Effect of (A) ethanol concentration (extraction time 60 min, extraction temperature 60°C), (B) extraction time (40% ethanol, extraction temperature 60°C), (C) extraction temperature (40% ethanol, extraction time 80 min) on TPC, TFC, DPPH and ABTS radical scavenging capacity from C.cyrtophyllum leaf extracts.

Mentions: Ethanol concentrations of 0%, 20%, 40%, 60%, 80% and 100% have been used extraction. Fig. 1A depicts of phenolic yield extracted (TPC and TFC) and the antioxidant capacities of those agents (against ABTS and DPPH radicals), and shows that both were greatly influenced by ethanol concentration. TPC and TFC recovery was parabolic with a maximum value at 60% ethanol. This was followed by a considerable decline with greater concentrations of ethanol. ABTS radical-scavenging capacities initially increased and peaked at 40% ethanol, then decreased considerably. DPPH radical-scavenging capacity declined with further increases in ethanol after the maximum values, 40% and 80% ethanol, respectively, were reached.


Optimization of ultrasonic-assisted extraction and radical-scavenging capacity of phenols and flavonoids from Clerodendrum cyrtophyllum Turcz leaves.

Zhou J, Zheng X, Yang Q, Liang Z, Li D, Yang X, Xu J - PLoS ONE (2013)

Effect of (A) ethanol concentration (extraction time 60 min, extraction temperature 60°C), (B) extraction time (40% ethanol, extraction temperature 60°C), (C) extraction temperature (40% ethanol, extraction time 80 min) on TPC, TFC, DPPH and ABTS radical scavenging capacity from C.cyrtophyllum leaf extracts.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3713038&req=5

pone-0068392-g001: Effect of (A) ethanol concentration (extraction time 60 min, extraction temperature 60°C), (B) extraction time (40% ethanol, extraction temperature 60°C), (C) extraction temperature (40% ethanol, extraction time 80 min) on TPC, TFC, DPPH and ABTS radical scavenging capacity from C.cyrtophyllum leaf extracts.
Mentions: Ethanol concentrations of 0%, 20%, 40%, 60%, 80% and 100% have been used extraction. Fig. 1A depicts of phenolic yield extracted (TPC and TFC) and the antioxidant capacities of those agents (against ABTS and DPPH radicals), and shows that both were greatly influenced by ethanol concentration. TPC and TFC recovery was parabolic with a maximum value at 60% ethanol. This was followed by a considerable decline with greater concentrations of ethanol. ABTS radical-scavenging capacities initially increased and peaked at 40% ethanol, then decreased considerably. DPPH radical-scavenging capacity declined with further increases in ethanol after the maximum values, 40% and 80% ethanol, respectively, were reached.

Bottom Line: Optimized extraction conditions for UAE from C. cyrtophyllum leaves were as follows: 60.9% ethanol, 85.4 min, and 63.3°C for maximal TPC extraction (16.8 ± 0.2 mg GAE/g DW); 67.7% ethanol, 82.9 min, and 63.0 °C for maximal TFC extraction (49.3 ± 0.4 mg RT/g DW); 48.8% ethanol, 85.1 min, and 63.9 °C for maximal DPPH radical-scavenging capacity (86.8 ± 0.2%); and 50.6% ethanol, 81.3 min, and 63.4 °C for maximal ABTS radical-scavenging capacity (92.9 ± 0.5%).Ethanol concentration was the most important factor in the extraction process.Our work offers optimal extraction conditions for C. cyrtophyllum as a potential source of natural antioxidants.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Protection and Development Utilization of Tropical Crop Germplasm Resources, Ministry of Education, College of Material and Chemical Engineering, Hainan University, Haikou, PR China.

ABSTRACT
Ultrasonic-assisted extraction (UAE) was developed to extract phenolic and flavonoid antioxidants from Clerodendrum cyrtophyllum Turcz leaves. The optimal experimental parameters for antioxidant extraction from C. cyrtophyllum leaves were measured using single-factor experimentation combined with response surface methodology (RSM). Total phenolic content (TPC) and total flavonoid content (TFC) assays were used to quantify antioxidant compounds. Next, antioxidant radical scavenging capacity was measured using 2,2'-diphenyl-1-picrylhydrazyl (DPPH) and 2,2' -azino-bis(3-ethylbenzothiazoline-6-sulphonicacid) (ABTS) radicals. Optimized extraction conditions for UAE from C. cyrtophyllum leaves were as follows: 60.9% ethanol, 85.4 min, and 63.3°C for maximal TPC extraction (16.8 ± 0.2 mg GAE/g DW); 67.7% ethanol, 82.9 min, and 63.0 °C for maximal TFC extraction (49.3 ± 0.4 mg RT/g DW); 48.8% ethanol, 85.1 min, and 63.9 °C for maximal DPPH radical-scavenging capacity (86.8 ± 0.2%); and 50.6% ethanol, 81.3 min, and 63.4 °C for maximal ABTS radical-scavenging capacity (92.9 ± 0.5%). Ethanol concentration was the most important factor in the extraction process. Our work offers optimal extraction conditions for C. cyrtophyllum as a potential source of natural antioxidants.

Show MeSH