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Identification and characterization of two novel viruses in ocular infections in reindeer.

Smits SL, Schapendonk CM, van Leeuwen M, Kuiken T, Bodewes R, Stalin Raj V, Haagmans BL, das Neves CG, Tryland M, Osterhaus AD - PLoS ONE (2013)

Bottom Line: A thorough understanding of virus diversity in wildlife provides epidemiological baseline information about pathogens.In this study, eye swab samples were obtained from semi-domesticated reindeer (Rangifertarandus tarandus) in Norway during an outbreak of infectious eye disease, possibly a very early stage of infectious keratoconjunctivitis (IKC).Whether either virus was responsible for causing the clinical signs or in any respect was associated to the disease condition remains to be determined.

View Article: PubMed Central - PubMed

Affiliation: ViroClinics BioSciences BV, Rotterdam, The Netherlands.

ABSTRACT
A thorough understanding of virus diversity in wildlife provides epidemiological baseline information about pathogens. In this study, eye swab samples were obtained from semi-domesticated reindeer (Rangifertarandus tarandus) in Norway during an outbreak of infectious eye disease, possibly a very early stage of infectious keratoconjunctivitis (IKC). Large scale molecular virus screening, based on host nucleic acid depletion, sequence-independent amplification and next-generation sequencing of partially purified viral nucleic acid, revealed the presence of a new papillomavirus in 2 out of 8 eye swab samples and a new betaherpesvirus in 3 out of 8 eye swab samples collected from animals with clinical signs and not in similar samples in 9 animals without clinical signs. Whether either virus was responsible for causing the clinical signs or in any respect was associated to the disease condition remains to be determined.

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Phylogram of nucleotide sequence data for a ~400-bp genome fragment from cervid herpesvirus 3 (corresponding to nt 61499-61904 of human herpesvirus strain RK; GenBank AF037218) compared with data for the orthologous regions of selected representative mammalian herpesviruses.The phylogenetic tree was generated with MEGA5, with the neighbor joining method and the p-distance model. Bar, evolutionary distance of 0.05. Bootstrap values (1000 reiterations) are shown. The different herpesvirus genera are indicated. Human herpesvirus 5, NC_006273; Panine herpesvirus 2, AF480884; Aotine herpesvirus 1, FJ483970; Tupaia herpesvirus 2, AF281817; Murid herpesvirus 1, HE610456:.Caviid herpesvirus 2, AB592928; Human herpesvirus 6A, X83413; Human herpesvirus 6B, AF157706; Human herpesvirus 7, AF037218; Elephantid herpesvirus 1, AAF322977; Cervid herpesvirus 3, KC810014.
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pone-0069711-g003: Phylogram of nucleotide sequence data for a ~400-bp genome fragment from cervid herpesvirus 3 (corresponding to nt 61499-61904 of human herpesvirus strain RK; GenBank AF037218) compared with data for the orthologous regions of selected representative mammalian herpesviruses.The phylogenetic tree was generated with MEGA5, with the neighbor joining method and the p-distance model. Bar, evolutionary distance of 0.05. Bootstrap values (1000 reiterations) are shown. The different herpesvirus genera are indicated. Human herpesvirus 5, NC_006273; Panine herpesvirus 2, AF480884; Aotine herpesvirus 1, FJ483970; Tupaia herpesvirus 2, AF281817; Murid herpesvirus 1, HE610456:.Caviid herpesvirus 2, AB592928; Human herpesvirus 6A, X83413; Human herpesvirus 6B, AF157706; Human herpesvirus 7, AF037218; Elephantid herpesvirus 1, AAF322977; Cervid herpesvirus 3, KC810014.

Mentions: Using large-scale molecular virus screening, herpesvirus-like sequences were detected in an eye swab of clinically affected reindeer 2 (Table 1). Using BLASTx, these sequences showed homology to human herpesvirus 7 (HHV7), which belongs to the subfamily Betaherpesvirinae. Thus, this reindeer herpesvirus was different from the previously identified cervid alphaherpesviruses 1 and 2 from deer and reindeer, respectively [16] and was designated cervid herpesvirus 3 (CvHV3). A phylogenetic tree was constructed based on a ~400 bp sequence fragment of an eye swab from reindeer 2 with ~65% identity to nucleotides 61499-61904 of HHV7 strain RK (AF037218; gene U40), encoding a subunit of terminase (herpesvirus core gene UL28 family; similar to HHV5 UL56) and the corresponding sequences of representative betaherpesviruses (Figure 3). The phylogenetic tree showed that CvHV3 was most closely related to HHV7 and belonged to the genus Roseolovirus.


Identification and characterization of two novel viruses in ocular infections in reindeer.

Smits SL, Schapendonk CM, van Leeuwen M, Kuiken T, Bodewes R, Stalin Raj V, Haagmans BL, das Neves CG, Tryland M, Osterhaus AD - PLoS ONE (2013)

Phylogram of nucleotide sequence data for a ~400-bp genome fragment from cervid herpesvirus 3 (corresponding to nt 61499-61904 of human herpesvirus strain RK; GenBank AF037218) compared with data for the orthologous regions of selected representative mammalian herpesviruses.The phylogenetic tree was generated with MEGA5, with the neighbor joining method and the p-distance model. Bar, evolutionary distance of 0.05. Bootstrap values (1000 reiterations) are shown. The different herpesvirus genera are indicated. Human herpesvirus 5, NC_006273; Panine herpesvirus 2, AF480884; Aotine herpesvirus 1, FJ483970; Tupaia herpesvirus 2, AF281817; Murid herpesvirus 1, HE610456:.Caviid herpesvirus 2, AB592928; Human herpesvirus 6A, X83413; Human herpesvirus 6B, AF157706; Human herpesvirus 7, AF037218; Elephantid herpesvirus 1, AAF322977; Cervid herpesvirus 3, KC810014.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3713034&req=5

pone-0069711-g003: Phylogram of nucleotide sequence data for a ~400-bp genome fragment from cervid herpesvirus 3 (corresponding to nt 61499-61904 of human herpesvirus strain RK; GenBank AF037218) compared with data for the orthologous regions of selected representative mammalian herpesviruses.The phylogenetic tree was generated with MEGA5, with the neighbor joining method and the p-distance model. Bar, evolutionary distance of 0.05. Bootstrap values (1000 reiterations) are shown. The different herpesvirus genera are indicated. Human herpesvirus 5, NC_006273; Panine herpesvirus 2, AF480884; Aotine herpesvirus 1, FJ483970; Tupaia herpesvirus 2, AF281817; Murid herpesvirus 1, HE610456:.Caviid herpesvirus 2, AB592928; Human herpesvirus 6A, X83413; Human herpesvirus 6B, AF157706; Human herpesvirus 7, AF037218; Elephantid herpesvirus 1, AAF322977; Cervid herpesvirus 3, KC810014.
Mentions: Using large-scale molecular virus screening, herpesvirus-like sequences were detected in an eye swab of clinically affected reindeer 2 (Table 1). Using BLASTx, these sequences showed homology to human herpesvirus 7 (HHV7), which belongs to the subfamily Betaherpesvirinae. Thus, this reindeer herpesvirus was different from the previously identified cervid alphaherpesviruses 1 and 2 from deer and reindeer, respectively [16] and was designated cervid herpesvirus 3 (CvHV3). A phylogenetic tree was constructed based on a ~400 bp sequence fragment of an eye swab from reindeer 2 with ~65% identity to nucleotides 61499-61904 of HHV7 strain RK (AF037218; gene U40), encoding a subunit of terminase (herpesvirus core gene UL28 family; similar to HHV5 UL56) and the corresponding sequences of representative betaherpesviruses (Figure 3). The phylogenetic tree showed that CvHV3 was most closely related to HHV7 and belonged to the genus Roseolovirus.

Bottom Line: A thorough understanding of virus diversity in wildlife provides epidemiological baseline information about pathogens.In this study, eye swab samples were obtained from semi-domesticated reindeer (Rangifertarandus tarandus) in Norway during an outbreak of infectious eye disease, possibly a very early stage of infectious keratoconjunctivitis (IKC).Whether either virus was responsible for causing the clinical signs or in any respect was associated to the disease condition remains to be determined.

View Article: PubMed Central - PubMed

Affiliation: ViroClinics BioSciences BV, Rotterdam, The Netherlands.

ABSTRACT
A thorough understanding of virus diversity in wildlife provides epidemiological baseline information about pathogens. In this study, eye swab samples were obtained from semi-domesticated reindeer (Rangifertarandus tarandus) in Norway during an outbreak of infectious eye disease, possibly a very early stage of infectious keratoconjunctivitis (IKC). Large scale molecular virus screening, based on host nucleic acid depletion, sequence-independent amplification and next-generation sequencing of partially purified viral nucleic acid, revealed the presence of a new papillomavirus in 2 out of 8 eye swab samples and a new betaherpesvirus in 3 out of 8 eye swab samples collected from animals with clinical signs and not in similar samples in 9 animals without clinical signs. Whether either virus was responsible for causing the clinical signs or in any respect was associated to the disease condition remains to be determined.

Show MeSH
Related in: MedlinePlus