Limits...
Differences in Cellulosic Supramolecular Structure of Compositionally Similar Rice Straw Affect Biomass Metabolism by Paddy Soil Microbiota.

Ogura T, Date Y, Kikuchi J - PLoS ONE (2013)

Bottom Line: We used a range of techniques including solid- and solution-state nuclear magnetic resonance (NMR) and Fourier transform infrared spectroscopy followed by thermodynamic and microbial degradability characterization using thermogravimetric analysis, solution-state NMR, and denaturing gradient gel electrophoresis.These measured data were further analyzed using an "ECOMICS" web-based toolkit.From the results, we found that physical pretreatment of rice straw alters the lignocellulosic supramolecular structure by cleaving significant molecular lignocellulose bonds.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Medical Life Science, Yokohama City University, Yokohama, Kanagawa, Japan.

ABSTRACT
Because they are strong and stable, lignocellulosic supramolecular structures in plant cell walls are resistant to decomposition. However, they can be degraded and recycled by soil microbiota. Little is known about the biomass degradation profiles of complex microbiota based on differences in cellulosic supramolecular structures without compositional variations. Here, we characterized and evaluated the cellulosic supramolecular structures and composition of rice straw biomass processed under different milling conditions. We used a range of techniques including solid- and solution-state nuclear magnetic resonance (NMR) and Fourier transform infrared spectroscopy followed by thermodynamic and microbial degradability characterization using thermogravimetric analysis, solution-state NMR, and denaturing gradient gel electrophoresis. These measured data were further analyzed using an "ECOMICS" web-based toolkit. From the results, we found that physical pretreatment of rice straw alters the lignocellulosic supramolecular structure by cleaving significant molecular lignocellulose bonds. The transformation from crystalline to amorphous cellulose shifted the thermal degradation profiles to lower temperatures. In addition, pretreated rice straw samples developed different microbiota profiles with different metabolic dynamics during the biomass degradation process. This is the first report to comprehensively characterize the structure, composition, and thermal degradation and microbiota profiles using the ECOMICS toolkit. By revealing differences between lignocellulosic supramolecular structures of biomass processed under different milling conditions, our analysis revealed how the characteristic compositions of microbiota profiles develop in addition to their metabolic profiles and dynamics during biomass degradation.

No MeSH data available.


Related in: MedlinePlus

Compositional characterization of biomass observed in 1H-13C HSQC spectra.1H-13C HSQC spectra of BM1- (A) and BM2-processed samples (B), and their ratios (BM2/BM1) of peak intensity (C). *, not detected in BM1-processed samples; α-D-Glcp, α-D-glucopyranoside; β-D-Glcp, β-D-glucopyranoside; β-D-Xylp, β-D-xylopyranoside; α-L-Araf, α-L-arabinofuranoside; α-L-Fucp, α-L-fucopyranoside; 2-O-Ac-β-D-Xylp, acetylated β-D-Xylp; X1γ, γ-position of cinnamyl alcohol end group; S, syringyl; G, guaiacyl; H, p-hydroxyphenyl; pCA, p-coumarate.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3686774&req=5

pone-0066919-g005: Compositional characterization of biomass observed in 1H-13C HSQC spectra.1H-13C HSQC spectra of BM1- (A) and BM2-processed samples (B), and their ratios (BM2/BM1) of peak intensity (C). *, not detected in BM1-processed samples; α-D-Glcp, α-D-glucopyranoside; β-D-Glcp, β-D-glucopyranoside; β-D-Xylp, β-D-xylopyranoside; α-L-Araf, α-L-arabinofuranoside; α-L-Fucp, α-L-fucopyranoside; 2-O-Ac-β-D-Xylp, acetylated β-D-Xylp; X1γ, γ-position of cinnamyl alcohol end group; S, syringyl; G, guaiacyl; H, p-hydroxyphenyl; pCA, p-coumarate.

Mentions: 1H- and 1H-13C HSQC spectra of biomass samples extracted using DMSO/pyridine were analyzed (Figs. 4 and 5). 1H-NMR spectral data were digitized using FT2DB and evaluated using PCA. AM-processed samples contributed to the positive direction of PC2, whereas BM-processed samples contributed to the positive directions of PC1 and PC2 (Fig. 4A). This separation was related to the low solubility of FM- and AM-processed samples in the DMSO/pyridine solvent compared with BM-processed samples (Fig. 4B). In addition, many peaks were annotated as lignocellulosic components such as acetyl (2.1 ppm), β-D-xylopyranoside (β-D-Xylp; 4.46 ppm and 5.04 ppm), β-O-4-p-hydroxyphenyl/guaiacyl (β-O-4-H/G; 4.96 ppm), α-L-fucopyranoside (Fucp; 5.36 ppm), α-L-arabinofuranoside (α-L-Araf; 5.64 ppm), and p-coumarate (pCA; 6.94 ppm).


Differences in Cellulosic Supramolecular Structure of Compositionally Similar Rice Straw Affect Biomass Metabolism by Paddy Soil Microbiota.

Ogura T, Date Y, Kikuchi J - PLoS ONE (2013)

Compositional characterization of biomass observed in 1H-13C HSQC spectra.1H-13C HSQC spectra of BM1- (A) and BM2-processed samples (B), and their ratios (BM2/BM1) of peak intensity (C). *, not detected in BM1-processed samples; α-D-Glcp, α-D-glucopyranoside; β-D-Glcp, β-D-glucopyranoside; β-D-Xylp, β-D-xylopyranoside; α-L-Araf, α-L-arabinofuranoside; α-L-Fucp, α-L-fucopyranoside; 2-O-Ac-β-D-Xylp, acetylated β-D-Xylp; X1γ, γ-position of cinnamyl alcohol end group; S, syringyl; G, guaiacyl; H, p-hydroxyphenyl; pCA, p-coumarate.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3686774&req=5

pone-0066919-g005: Compositional characterization of biomass observed in 1H-13C HSQC spectra.1H-13C HSQC spectra of BM1- (A) and BM2-processed samples (B), and their ratios (BM2/BM1) of peak intensity (C). *, not detected in BM1-processed samples; α-D-Glcp, α-D-glucopyranoside; β-D-Glcp, β-D-glucopyranoside; β-D-Xylp, β-D-xylopyranoside; α-L-Araf, α-L-arabinofuranoside; α-L-Fucp, α-L-fucopyranoside; 2-O-Ac-β-D-Xylp, acetylated β-D-Xylp; X1γ, γ-position of cinnamyl alcohol end group; S, syringyl; G, guaiacyl; H, p-hydroxyphenyl; pCA, p-coumarate.
Mentions: 1H- and 1H-13C HSQC spectra of biomass samples extracted using DMSO/pyridine were analyzed (Figs. 4 and 5). 1H-NMR spectral data were digitized using FT2DB and evaluated using PCA. AM-processed samples contributed to the positive direction of PC2, whereas BM-processed samples contributed to the positive directions of PC1 and PC2 (Fig. 4A). This separation was related to the low solubility of FM- and AM-processed samples in the DMSO/pyridine solvent compared with BM-processed samples (Fig. 4B). In addition, many peaks were annotated as lignocellulosic components such as acetyl (2.1 ppm), β-D-xylopyranoside (β-D-Xylp; 4.46 ppm and 5.04 ppm), β-O-4-p-hydroxyphenyl/guaiacyl (β-O-4-H/G; 4.96 ppm), α-L-fucopyranoside (Fucp; 5.36 ppm), α-L-arabinofuranoside (α-L-Araf; 5.64 ppm), and p-coumarate (pCA; 6.94 ppm).

Bottom Line: We used a range of techniques including solid- and solution-state nuclear magnetic resonance (NMR) and Fourier transform infrared spectroscopy followed by thermodynamic and microbial degradability characterization using thermogravimetric analysis, solution-state NMR, and denaturing gradient gel electrophoresis.These measured data were further analyzed using an "ECOMICS" web-based toolkit.From the results, we found that physical pretreatment of rice straw alters the lignocellulosic supramolecular structure by cleaving significant molecular lignocellulose bonds.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Medical Life Science, Yokohama City University, Yokohama, Kanagawa, Japan.

ABSTRACT
Because they are strong and stable, lignocellulosic supramolecular structures in plant cell walls are resistant to decomposition. However, they can be degraded and recycled by soil microbiota. Little is known about the biomass degradation profiles of complex microbiota based on differences in cellulosic supramolecular structures without compositional variations. Here, we characterized and evaluated the cellulosic supramolecular structures and composition of rice straw biomass processed under different milling conditions. We used a range of techniques including solid- and solution-state nuclear magnetic resonance (NMR) and Fourier transform infrared spectroscopy followed by thermodynamic and microbial degradability characterization using thermogravimetric analysis, solution-state NMR, and denaturing gradient gel electrophoresis. These measured data were further analyzed using an "ECOMICS" web-based toolkit. From the results, we found that physical pretreatment of rice straw alters the lignocellulosic supramolecular structure by cleaving significant molecular lignocellulose bonds. The transformation from crystalline to amorphous cellulose shifted the thermal degradation profiles to lower temperatures. In addition, pretreated rice straw samples developed different microbiota profiles with different metabolic dynamics during the biomass degradation process. This is the first report to comprehensively characterize the structure, composition, and thermal degradation and microbiota profiles using the ECOMICS toolkit. By revealing differences between lignocellulosic supramolecular structures of biomass processed under different milling conditions, our analysis revealed how the characteristic compositions of microbiota profiles develop in addition to their metabolic profiles and dynamics during biomass degradation.

No MeSH data available.


Related in: MedlinePlus