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Differences in Cellulosic Supramolecular Structure of Compositionally Similar Rice Straw Affect Biomass Metabolism by Paddy Soil Microbiota.

Ogura T, Date Y, Kikuchi J - PLoS ONE (2013)

Bottom Line: We used a range of techniques including solid- and solution-state nuclear magnetic resonance (NMR) and Fourier transform infrared spectroscopy followed by thermodynamic and microbial degradability characterization using thermogravimetric analysis, solution-state NMR, and denaturing gradient gel electrophoresis.These measured data were further analyzed using an "ECOMICS" web-based toolkit.From the results, we found that physical pretreatment of rice straw alters the lignocellulosic supramolecular structure by cleaving significant molecular lignocellulose bonds.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Medical Life Science, Yokohama City University, Yokohama, Kanagawa, Japan.

ABSTRACT
Because they are strong and stable, lignocellulosic supramolecular structures in plant cell walls are resistant to decomposition. However, they can be degraded and recycled by soil microbiota. Little is known about the biomass degradation profiles of complex microbiota based on differences in cellulosic supramolecular structures without compositional variations. Here, we characterized and evaluated the cellulosic supramolecular structures and composition of rice straw biomass processed under different milling conditions. We used a range of techniques including solid- and solution-state nuclear magnetic resonance (NMR) and Fourier transform infrared spectroscopy followed by thermodynamic and microbial degradability characterization using thermogravimetric analysis, solution-state NMR, and denaturing gradient gel electrophoresis. These measured data were further analyzed using an "ECOMICS" web-based toolkit. From the results, we found that physical pretreatment of rice straw alters the lignocellulosic supramolecular structure by cleaving significant molecular lignocellulose bonds. The transformation from crystalline to amorphous cellulose shifted the thermal degradation profiles to lower temperatures. In addition, pretreated rice straw samples developed different microbiota profiles with different metabolic dynamics during the biomass degradation process. This is the first report to comprehensively characterize the structure, composition, and thermal degradation and microbiota profiles using the ECOMICS toolkit. By revealing differences between lignocellulosic supramolecular structures of biomass processed under different milling conditions, our analysis revealed how the characteristic compositions of microbiota profiles develop in addition to their metabolic profiles and dynamics during biomass degradation.

No MeSH data available.


Related in: MedlinePlus

Schematic overview of this study.The effects of rice straw pretreatment on the cellulosic supramolecular structure and improvements in digestibility of lignocellulosic biomass for paddy soil microbiota were evaluated by physicochemical and biochemical methods. Rice straw samples were powdered using a blender, AM machine, and BM machine (1). Structural, compositional, thermodynamic, and degradability characterization (from 2–1 to 2–4) were performed using multimeasurement techniques such as FTIR, NMR, TG/DTA, and DGGE fingerprinting. Data were analyzed using the ECOMICS web-based toolkit.
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pone-0066919-g001: Schematic overview of this study.The effects of rice straw pretreatment on the cellulosic supramolecular structure and improvements in digestibility of lignocellulosic biomass for paddy soil microbiota were evaluated by physicochemical and biochemical methods. Rice straw samples were powdered using a blender, AM machine, and BM machine (1). Structural, compositional, thermodynamic, and degradability characterization (from 2–1 to 2–4) were performed using multimeasurement techniques such as FTIR, NMR, TG/DTA, and DGGE fingerprinting. Data were analyzed using the ECOMICS web-based toolkit.

Mentions: This study focused on the effects of rice straw pretreatment on the cellulosic supramolecular structure and aimed to improve digestibility of lignocellulosic biomass for paddy soil microbiota. Supramolecular structures and composition of biomass were characterized using multiphysicochemical approaches combined with the ECOMICS web tools for multivariate data analysis (Fig. 1). Paddy soil microbiota was classified using E-class. Subsequently, the biomass degradation profiles of paddy soil microbiota were evaluated.


Differences in Cellulosic Supramolecular Structure of Compositionally Similar Rice Straw Affect Biomass Metabolism by Paddy Soil Microbiota.

Ogura T, Date Y, Kikuchi J - PLoS ONE (2013)

Schematic overview of this study.The effects of rice straw pretreatment on the cellulosic supramolecular structure and improvements in digestibility of lignocellulosic biomass for paddy soil microbiota were evaluated by physicochemical and biochemical methods. Rice straw samples were powdered using a blender, AM machine, and BM machine (1). Structural, compositional, thermodynamic, and degradability characterization (from 2–1 to 2–4) were performed using multimeasurement techniques such as FTIR, NMR, TG/DTA, and DGGE fingerprinting. Data were analyzed using the ECOMICS web-based toolkit.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3686774&req=5

pone-0066919-g001: Schematic overview of this study.The effects of rice straw pretreatment on the cellulosic supramolecular structure and improvements in digestibility of lignocellulosic biomass for paddy soil microbiota were evaluated by physicochemical and biochemical methods. Rice straw samples were powdered using a blender, AM machine, and BM machine (1). Structural, compositional, thermodynamic, and degradability characterization (from 2–1 to 2–4) were performed using multimeasurement techniques such as FTIR, NMR, TG/DTA, and DGGE fingerprinting. Data were analyzed using the ECOMICS web-based toolkit.
Mentions: This study focused on the effects of rice straw pretreatment on the cellulosic supramolecular structure and aimed to improve digestibility of lignocellulosic biomass for paddy soil microbiota. Supramolecular structures and composition of biomass were characterized using multiphysicochemical approaches combined with the ECOMICS web tools for multivariate data analysis (Fig. 1). Paddy soil microbiota was classified using E-class. Subsequently, the biomass degradation profiles of paddy soil microbiota were evaluated.

Bottom Line: We used a range of techniques including solid- and solution-state nuclear magnetic resonance (NMR) and Fourier transform infrared spectroscopy followed by thermodynamic and microbial degradability characterization using thermogravimetric analysis, solution-state NMR, and denaturing gradient gel electrophoresis.These measured data were further analyzed using an "ECOMICS" web-based toolkit.From the results, we found that physical pretreatment of rice straw alters the lignocellulosic supramolecular structure by cleaving significant molecular lignocellulose bonds.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Medical Life Science, Yokohama City University, Yokohama, Kanagawa, Japan.

ABSTRACT
Because they are strong and stable, lignocellulosic supramolecular structures in plant cell walls are resistant to decomposition. However, they can be degraded and recycled by soil microbiota. Little is known about the biomass degradation profiles of complex microbiota based on differences in cellulosic supramolecular structures without compositional variations. Here, we characterized and evaluated the cellulosic supramolecular structures and composition of rice straw biomass processed under different milling conditions. We used a range of techniques including solid- and solution-state nuclear magnetic resonance (NMR) and Fourier transform infrared spectroscopy followed by thermodynamic and microbial degradability characterization using thermogravimetric analysis, solution-state NMR, and denaturing gradient gel electrophoresis. These measured data were further analyzed using an "ECOMICS" web-based toolkit. From the results, we found that physical pretreatment of rice straw alters the lignocellulosic supramolecular structure by cleaving significant molecular lignocellulose bonds. The transformation from crystalline to amorphous cellulose shifted the thermal degradation profiles to lower temperatures. In addition, pretreated rice straw samples developed different microbiota profiles with different metabolic dynamics during the biomass degradation process. This is the first report to comprehensively characterize the structure, composition, and thermal degradation and microbiota profiles using the ECOMICS toolkit. By revealing differences between lignocellulosic supramolecular structures of biomass processed under different milling conditions, our analysis revealed how the characteristic compositions of microbiota profiles develop in addition to their metabolic profiles and dynamics during biomass degradation.

No MeSH data available.


Related in: MedlinePlus