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Comparative Genomic Analyses of Streptococcus pseudopneumoniae Provide Insight into Virulence and Commensalism Dynamics.

Shahinas D, Thornton CS, Tamber GS, Arya G, Wong A, Jamieson FB, Ma JH, Alexander DC, Low DE, Pillai DR - PLoS ONE (2013)

Bottom Line: Although the pathogenic potential of S. pseudopneumoniae remains uncertain, it is most commonly isolated from patients with underlying medical conditions, such as chronic obstructive pulmonary disease.Analysis of gene content reveals numerous unique features that distinguish S. pseudopneumoniae from other streptococci.Additionally, the presence of several virulence factors and antibiotic resistance mechanisms suggest the potential of this commensal species to become pathogenic or to contribute to increasing antibiotic resistance levels seen among the VGS.

View Article: PubMed Central - PubMed

Affiliation: Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Canada.

ABSTRACT
Streptococcus pseudopneumoniae (SPPN) is a recently described species of the viridans group streptococci (VGS). Although the pathogenic potential of S. pseudopneumoniae remains uncertain, it is most commonly isolated from patients with underlying medical conditions, such as chronic obstructive pulmonary disease. S. pseudopneumoniae can be distinguished from the closely related species, S. pneumoniae and S. mitis, by phenotypic characteristics, including optochin resistance in the presence of 5% CO2, bile insolubility, and the lack of the pneumococcal capsule. Previously, we reported the draft genome sequence of S. pseudopneumoniae IS7493, a clinical isolate obtained from an immunocompromised patient with documented pneumonia. Here, we use comparative genomics approaches to identify similarities and key differences between S. pseudopneumoniae IS7493, S. pneumoniae and S. mitis. The genome structure of S. pseudopneumoniae IS7493 is most closely related to that of S. pneumoniae R6, but several recombination events are evident. Analysis of gene content reveals numerous unique features that distinguish S. pseudopneumoniae from other streptococci. The presence of loci for competence, iron transport, pneumolysin production and antimicrobial resistance reinforce the phylogenetic position of S. pseudopneumoniae as an intermediate species between S. pneumoniae and S. mitis. Additionally, the presence of several virulence factors and antibiotic resistance mechanisms suggest the potential of this commensal species to become pathogenic or to contribute to increasing antibiotic resistance levels seen among the VGS.

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Related in: MedlinePlus

Overview of the genome of S.pseudopneumoniae IS7493.(a) Representation of the S. pseudopneumoniae IS7493 circular genome. The inner circles represent GC content (outer) and GC bias (innermost). (b) Full assembly revealed the presence of plasmid pDRPIS7493.
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pone-0065670-g001: Overview of the genome of S.pseudopneumoniae IS7493.(a) Representation of the S. pseudopneumoniae IS7493 circular genome. The inner circles represent GC content (outer) and GC bias (innermost). (b) Full assembly revealed the presence of plasmid pDRPIS7493.

Mentions: General features of the S. pseudopneumoniae IS7493 genome and assembly statistics are listed in Table S1. In addition to a single circular (2,190,731 bp; 39.8% GC content) chromosomal genome (Figure 1a), isolate IS7493 also harbors a single plasmid of 4.7 kb (38.3% GC content), which was conventionally assigned as pDRPIS7493 (Figure 1b).


Comparative Genomic Analyses of Streptococcus pseudopneumoniae Provide Insight into Virulence and Commensalism Dynamics.

Shahinas D, Thornton CS, Tamber GS, Arya G, Wong A, Jamieson FB, Ma JH, Alexander DC, Low DE, Pillai DR - PLoS ONE (2013)

Overview of the genome of S.pseudopneumoniae IS7493.(a) Representation of the S. pseudopneumoniae IS7493 circular genome. The inner circles represent GC content (outer) and GC bias (innermost). (b) Full assembly revealed the presence of plasmid pDRPIS7493.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3686770&req=5

pone-0065670-g001: Overview of the genome of S.pseudopneumoniae IS7493.(a) Representation of the S. pseudopneumoniae IS7493 circular genome. The inner circles represent GC content (outer) and GC bias (innermost). (b) Full assembly revealed the presence of plasmid pDRPIS7493.
Mentions: General features of the S. pseudopneumoniae IS7493 genome and assembly statistics are listed in Table S1. In addition to a single circular (2,190,731 bp; 39.8% GC content) chromosomal genome (Figure 1a), isolate IS7493 also harbors a single plasmid of 4.7 kb (38.3% GC content), which was conventionally assigned as pDRPIS7493 (Figure 1b).

Bottom Line: Although the pathogenic potential of S. pseudopneumoniae remains uncertain, it is most commonly isolated from patients with underlying medical conditions, such as chronic obstructive pulmonary disease.Analysis of gene content reveals numerous unique features that distinguish S. pseudopneumoniae from other streptococci.Additionally, the presence of several virulence factors and antibiotic resistance mechanisms suggest the potential of this commensal species to become pathogenic or to contribute to increasing antibiotic resistance levels seen among the VGS.

View Article: PubMed Central - PubMed

Affiliation: Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Canada.

ABSTRACT
Streptococcus pseudopneumoniae (SPPN) is a recently described species of the viridans group streptococci (VGS). Although the pathogenic potential of S. pseudopneumoniae remains uncertain, it is most commonly isolated from patients with underlying medical conditions, such as chronic obstructive pulmonary disease. S. pseudopneumoniae can be distinguished from the closely related species, S. pneumoniae and S. mitis, by phenotypic characteristics, including optochin resistance in the presence of 5% CO2, bile insolubility, and the lack of the pneumococcal capsule. Previously, we reported the draft genome sequence of S. pseudopneumoniae IS7493, a clinical isolate obtained from an immunocompromised patient with documented pneumonia. Here, we use comparative genomics approaches to identify similarities and key differences between S. pseudopneumoniae IS7493, S. pneumoniae and S. mitis. The genome structure of S. pseudopneumoniae IS7493 is most closely related to that of S. pneumoniae R6, but several recombination events are evident. Analysis of gene content reveals numerous unique features that distinguish S. pseudopneumoniae from other streptococci. The presence of loci for competence, iron transport, pneumolysin production and antimicrobial resistance reinforce the phylogenetic position of S. pseudopneumoniae as an intermediate species between S. pneumoniae and S. mitis. Additionally, the presence of several virulence factors and antibiotic resistance mechanisms suggest the potential of this commensal species to become pathogenic or to contribute to increasing antibiotic resistance levels seen among the VGS.

No MeSH data available.


Related in: MedlinePlus