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Acetylation of Chromatin-Associated Histone H3 Lysine 56 Inhibits the Development of Encysted Artemia Embryos.

Zhou R, Yang F, Chen DF, Sun YX, Yang JS, Yang WJ - PLoS ONE (2013)

Bottom Line: We found that the level of H3K56ac on chromatin increased during diapause formation, and decreased upon diapause termination, remaining basal level throughout subsequent embryonic development.Furthermore, we found that this arrest of the cell cycle and development was induced by H3K56ac and dephosphorylation of the checkpoint protein, retinoblastoma protein.These results have revealed the dynamic change in H3K56ac on chromatin during artemia diapause formation and termination.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education and College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China.

ABSTRACT

Background: As a response to harsh environments, the crustacean artemia produces diapause gastrula embryos (cysts), in which cell division and embryonic development are totally arrested. This dormant state can last for very long periods but be terminated by specific environmental stimuli. Thus, artemia is an ideal model organism in which to study cell cycle arrest and embryonic development.

Principal finding: Our study focuses on the roles of H3K56ac in the arrest of cell cycle and development during artemia diapause formation and termination. We found that the level of H3K56ac on chromatin increased during diapause formation, and decreased upon diapause termination, remaining basal level throughout subsequent embryonic development. In both HeLa cells and artemia, blocking the deacetylation with nicotinamide, a histone deacetylase inhibitor, increased the level of H3K56ac on chromatin and induced an artificial cell cycle arrest. Furthermore, we found that this arrest of the cell cycle and development was induced by H3K56ac and dephosphorylation of the checkpoint protein, retinoblastoma protein.

Conclusions/significance: These results have revealed the dynamic change in H3K56ac on chromatin during artemia diapause formation and termination. Thus, our findings provide insight into the regulation of cell division during arrest of artemia embryonic development and provide further insight into the functions of H3K56ac.

No MeSH data available.


Related in: MedlinePlus

NM artificially arrests the cell cycle and development in post-diapause embryos.(A) BrdU incorporation assay of control and test groups in the NM treatment. Samples were treated and collected as described above. The black arrows indicate the representative positive signal. (B) Western blotting analysis. Tubulin and H3 were used as loading controls.
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pone-0068374-g006: NM artificially arrests the cell cycle and development in post-diapause embryos.(A) BrdU incorporation assay of control and test groups in the NM treatment. Samples were treated and collected as described above. The black arrows indicate the representative positive signal. (B) Western blotting analysis. Tubulin and H3 were used as loading controls.

Mentions: The BrdU incorporation assay and phosphorylation of H3S10 were used to indicate cell division during embryonic development. Cell division resumed at the emergence stage (12h in control), whereas did not occur in the arrested embryos (12-24h in test) after NM treatment (Figure 6A and 6B). Cell division resumed after NM is removed and the embryos developed into normal napulius larvae.


Acetylation of Chromatin-Associated Histone H3 Lysine 56 Inhibits the Development of Encysted Artemia Embryos.

Zhou R, Yang F, Chen DF, Sun YX, Yang JS, Yang WJ - PLoS ONE (2013)

NM artificially arrests the cell cycle and development in post-diapause embryos.(A) BrdU incorporation assay of control and test groups in the NM treatment. Samples were treated and collected as described above. The black arrows indicate the representative positive signal. (B) Western blotting analysis. Tubulin and H3 were used as loading controls.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3686719&req=5

pone-0068374-g006: NM artificially arrests the cell cycle and development in post-diapause embryos.(A) BrdU incorporation assay of control and test groups in the NM treatment. Samples were treated and collected as described above. The black arrows indicate the representative positive signal. (B) Western blotting analysis. Tubulin and H3 were used as loading controls.
Mentions: The BrdU incorporation assay and phosphorylation of H3S10 were used to indicate cell division during embryonic development. Cell division resumed at the emergence stage (12h in control), whereas did not occur in the arrested embryos (12-24h in test) after NM treatment (Figure 6A and 6B). Cell division resumed after NM is removed and the embryos developed into normal napulius larvae.

Bottom Line: We found that the level of H3K56ac on chromatin increased during diapause formation, and decreased upon diapause termination, remaining basal level throughout subsequent embryonic development.Furthermore, we found that this arrest of the cell cycle and development was induced by H3K56ac and dephosphorylation of the checkpoint protein, retinoblastoma protein.These results have revealed the dynamic change in H3K56ac on chromatin during artemia diapause formation and termination.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education and College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China.

ABSTRACT

Background: As a response to harsh environments, the crustacean artemia produces diapause gastrula embryos (cysts), in which cell division and embryonic development are totally arrested. This dormant state can last for very long periods but be terminated by specific environmental stimuli. Thus, artemia is an ideal model organism in which to study cell cycle arrest and embryonic development.

Principal finding: Our study focuses on the roles of H3K56ac in the arrest of cell cycle and development during artemia diapause formation and termination. We found that the level of H3K56ac on chromatin increased during diapause formation, and decreased upon diapause termination, remaining basal level throughout subsequent embryonic development. In both HeLa cells and artemia, blocking the deacetylation with nicotinamide, a histone deacetylase inhibitor, increased the level of H3K56ac on chromatin and induced an artificial cell cycle arrest. Furthermore, we found that this arrest of the cell cycle and development was induced by H3K56ac and dephosphorylation of the checkpoint protein, retinoblastoma protein.

Conclusions/significance: These results have revealed the dynamic change in H3K56ac on chromatin during artemia diapause formation and termination. Thus, our findings provide insight into the regulation of cell division during arrest of artemia embryonic development and provide further insight into the functions of H3K56ac.

No MeSH data available.


Related in: MedlinePlus