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Growth and potential damage of human bone-derived cells on fresh and aged fullerene c60 films.

Kopova I, Bacakova L, Lavrentiev V, Vacik J - Int J Mol Sci (2013)

Bottom Line: After seven days of cultivation, we did not observe any cytotoxic morphological changes, such as enlarged cells or cytosolic vacuole formation.The increasing age of the fullerene films did not cause enhancement of cytotoxicity.On the contrary, it resulted in an improvement in the properties of these materials, which are more suitable for cell cultivation.

View Article: PubMed Central - PubMed

Affiliation: Institute of Physiology, Academy of Sciences of the Czech Republic, Videnska 1083, 14220 Prague 4-Krc, Czech Republic. lucy@biomed.cas.cz.

ABSTRACT
Fullerenes are nanoparticles composed of carbon atoms arranged in a spherical hollow cage-like structure. Numerous studies have evaluated the therapeutic potential of fullerene derivates against oxidative stress-associated conditions, including the prevention or treatment of arthritis. On the other hand, fullerenes are not only able to quench, but also to generate harmful reactive oxygen species. The reactivity of fullerenes may change in time due to the oxidation and polymerization of fullerenes in an air atmosphere. In this study, we therefore tested the dependence between the age of fullerene films (from one week to one year) and the proliferation, viability and metabolic activity of human osteosarcoma cells (lines MG-63 and U-2 OS). We also monitored potential membrane and DNA damage and morphological changes of the cells. After seven days of cultivation, we did not observe any cytotoxic morphological changes, such as enlarged cells or cytosolic vacuole formation. Furthermore, there was no increased level of DNA damage. The increasing age of the fullerene films did not cause enhancement of cytotoxicity. On the contrary, it resulted in an improvement in the properties of these materials, which are more suitable for cell cultivation. Therefore, fullerene films could be considered as a promising material with potential use as a bioactive coating of cell carriers for bone tissue engineering.

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Related in: MedlinePlus

Height of the prominences and the grooves of the micropatterned fresh (A) and aged (B) C60 films.
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f2-ijms-14-09182: Height of the prominences and the grooves of the micropatterned fresh (A) and aged (B) C60 films.

Mentions: It is also possible to use AFM to scan and analyze the heights of the prominences and grooves. The measurements revealed, in the case of the fresh C60 films, that the prominences are about twice as high as the grooves (prominences = 53 nm, grooves = 25 nm), though in the case of the aged layers, the height of the C60 prominences are similar to the height of the grooves (prominences = 27 nm, grooves = 29 nm; Figure 2). This decrease in height of the prominences on aged C60 films can be explained by post-deposition surface diffusion of the fullerene molecules in time, i.e., during the aging period of fullerene films.


Growth and potential damage of human bone-derived cells on fresh and aged fullerene c60 films.

Kopova I, Bacakova L, Lavrentiev V, Vacik J - Int J Mol Sci (2013)

Height of the prominences and the grooves of the micropatterned fresh (A) and aged (B) C60 films.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3676779&req=5

f2-ijms-14-09182: Height of the prominences and the grooves of the micropatterned fresh (A) and aged (B) C60 films.
Mentions: It is also possible to use AFM to scan and analyze the heights of the prominences and grooves. The measurements revealed, in the case of the fresh C60 films, that the prominences are about twice as high as the grooves (prominences = 53 nm, grooves = 25 nm), though in the case of the aged layers, the height of the C60 prominences are similar to the height of the grooves (prominences = 27 nm, grooves = 29 nm; Figure 2). This decrease in height of the prominences on aged C60 films can be explained by post-deposition surface diffusion of the fullerene molecules in time, i.e., during the aging period of fullerene films.

Bottom Line: After seven days of cultivation, we did not observe any cytotoxic morphological changes, such as enlarged cells or cytosolic vacuole formation.The increasing age of the fullerene films did not cause enhancement of cytotoxicity.On the contrary, it resulted in an improvement in the properties of these materials, which are more suitable for cell cultivation.

View Article: PubMed Central - PubMed

Affiliation: Institute of Physiology, Academy of Sciences of the Czech Republic, Videnska 1083, 14220 Prague 4-Krc, Czech Republic. lucy@biomed.cas.cz.

ABSTRACT
Fullerenes are nanoparticles composed of carbon atoms arranged in a spherical hollow cage-like structure. Numerous studies have evaluated the therapeutic potential of fullerene derivates against oxidative stress-associated conditions, including the prevention or treatment of arthritis. On the other hand, fullerenes are not only able to quench, but also to generate harmful reactive oxygen species. The reactivity of fullerenes may change in time due to the oxidation and polymerization of fullerenes in an air atmosphere. In this study, we therefore tested the dependence between the age of fullerene films (from one week to one year) and the proliferation, viability and metabolic activity of human osteosarcoma cells (lines MG-63 and U-2 OS). We also monitored potential membrane and DNA damage and morphological changes of the cells. After seven days of cultivation, we did not observe any cytotoxic morphological changes, such as enlarged cells or cytosolic vacuole formation. Furthermore, there was no increased level of DNA damage. The increasing age of the fullerene films did not cause enhancement of cytotoxicity. On the contrary, it resulted in an improvement in the properties of these materials, which are more suitable for cell cultivation. Therefore, fullerene films could be considered as a promising material with potential use as a bioactive coating of cell carriers for bone tissue engineering.

Show MeSH
Related in: MedlinePlus