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Tumour cell labelling by magnetic nanoparticles with determination of intracellular iron content and spatial distribution of the intracellular iron.

Wang Z, Cuschieri A - Int J Mol Sci (2013)

Bottom Line: The material is also widely used for in vitro cell labelling, as are other SPIO-based particles.Our results on the uptake by human cancer cell lines of ferumoxides indicate that electroporation in the presence of protamine sulphate (PS) results in rapid high uptake of SPIO nanoparticles (SPIONs) by parenchymal tumour cells without significant impairment of cell viability.Quantitative determination of cellular iron uptake performed by colorimetric assay is in agreement with data from the literature.

View Article: PubMed Central - PubMed

Affiliation: Institute for Medical Science and Technology, University of Dundee, Dundee DD2 1FD, UK. z.z.wang@dundee.ac.uk.

ABSTRACT
Magnetically labelled cells are used for in vivo cell tracking by MRI, used for the clinical translation of cell-base therapies. Studies involving magnetic labelled cells may include separation of labelled cells, targeted delivery and controlled release of drugs, contrast enhanced MRI and magnetic hyperthermia for the in situ ablation of tumours. Dextran-coated super-paramagnetic iron oxide (SPIO) ferumoxides are used clinically as an MR contrast agents primarily for hepatic imaging. The material is also widely used for in vitro cell labelling, as are other SPIO-based particles. Our results on the uptake by human cancer cell lines of ferumoxides indicate that electroporation in the presence of protamine sulphate (PS) results in rapid high uptake of SPIO nanoparticles (SPIONs) by parenchymal tumour cells without significant impairment of cell viability. Quantitative determination of cellular iron uptake performed by colorimetric assay is in agreement with data from the literature. These results on intracellular iron content together with the intracellular distribution of SPIONs by magnetic force microscopy (MFM) following in vitro uptake by parenchymal tumour cells confirm the potential of this technique for clinical tumour cell detection and destruction.

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Cell labelling by electroporation and PS/electroporation: (a) stained promptly after electroporation (Cytospin staining); (b) stained after overnight culture. PS, protamine sulphate; EP, electroporation; IO-nPs, ferumoxides.
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f1-ijms-14-09111: Cell labelling by electroporation and PS/electroporation: (a) stained promptly after electroporation (Cytospin staining); (b) stained after overnight culture. PS, protamine sulphate; EP, electroporation; IO-nPs, ferumoxides.

Mentions: Cells were successfully labelled by magnetic nanoparticles (IO-nPs or ferumoxides) using Prussian blue staining, as illustrated (Figure 1). The resulting cell labelling efficiency and cell viability for three labelling methods used in this study are shown in Table 1. A very high labelling efficiency (95%) was obtained using protamine sulphate (PS) alone, while electroporation (EP) was only capable of loading cells with an efficiency of 72%. When PS was added to magnetic nanoparticles solution in electroporation mixtures, the loading efficiency increased from 72% to 88%.


Tumour cell labelling by magnetic nanoparticles with determination of intracellular iron content and spatial distribution of the intracellular iron.

Wang Z, Cuschieri A - Int J Mol Sci (2013)

Cell labelling by electroporation and PS/electroporation: (a) stained promptly after electroporation (Cytospin staining); (b) stained after overnight culture. PS, protamine sulphate; EP, electroporation; IO-nPs, ferumoxides.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3676776&req=5

f1-ijms-14-09111: Cell labelling by electroporation and PS/electroporation: (a) stained promptly after electroporation (Cytospin staining); (b) stained after overnight culture. PS, protamine sulphate; EP, electroporation; IO-nPs, ferumoxides.
Mentions: Cells were successfully labelled by magnetic nanoparticles (IO-nPs or ferumoxides) using Prussian blue staining, as illustrated (Figure 1). The resulting cell labelling efficiency and cell viability for three labelling methods used in this study are shown in Table 1. A very high labelling efficiency (95%) was obtained using protamine sulphate (PS) alone, while electroporation (EP) was only capable of loading cells with an efficiency of 72%. When PS was added to magnetic nanoparticles solution in electroporation mixtures, the loading efficiency increased from 72% to 88%.

Bottom Line: The material is also widely used for in vitro cell labelling, as are other SPIO-based particles.Our results on the uptake by human cancer cell lines of ferumoxides indicate that electroporation in the presence of protamine sulphate (PS) results in rapid high uptake of SPIO nanoparticles (SPIONs) by parenchymal tumour cells without significant impairment of cell viability.Quantitative determination of cellular iron uptake performed by colorimetric assay is in agreement with data from the literature.

View Article: PubMed Central - PubMed

Affiliation: Institute for Medical Science and Technology, University of Dundee, Dundee DD2 1FD, UK. z.z.wang@dundee.ac.uk.

ABSTRACT
Magnetically labelled cells are used for in vivo cell tracking by MRI, used for the clinical translation of cell-base therapies. Studies involving magnetic labelled cells may include separation of labelled cells, targeted delivery and controlled release of drugs, contrast enhanced MRI and magnetic hyperthermia for the in situ ablation of tumours. Dextran-coated super-paramagnetic iron oxide (SPIO) ferumoxides are used clinically as an MR contrast agents primarily for hepatic imaging. The material is also widely used for in vitro cell labelling, as are other SPIO-based particles. Our results on the uptake by human cancer cell lines of ferumoxides indicate that electroporation in the presence of protamine sulphate (PS) results in rapid high uptake of SPIO nanoparticles (SPIONs) by parenchymal tumour cells without significant impairment of cell viability. Quantitative determination of cellular iron uptake performed by colorimetric assay is in agreement with data from the literature. These results on intracellular iron content together with the intracellular distribution of SPIONs by magnetic force microscopy (MFM) following in vitro uptake by parenchymal tumour cells confirm the potential of this technique for clinical tumour cell detection and destruction.

Show MeSH
Related in: MedlinePlus