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Direct visualization of changes of lymphatic function and drainage pathways in lymph node metastasis of B16F10 melanoma using near-infrared fluorescence imaging.

Kwon S, Agollah GD, Wu G, Chan W, Sevick-Muraca EM - Biomed Opt Express (2013)

Bottom Line: We observed significant reduction of lymphatic contractility from 7 days p.i. until 21 days p.i..Since lymphatic function and architecture were progressively altered during tumor growth and metastasis, non-invasive NIRF imaging may provide a new method to stage disease.In addition, this novel technique can be used as a diagnostic method to non-invasively assess lymphatic response as mechanism of therapeutic action.

View Article: PubMed Central - PubMed

Affiliation: Center for Molecular Imaging, The Brown Foundation Institute of Molecular Medicine, The University of Texas Health Science Center, Houston, TX 77030, USA.

ABSTRACT
The lymphatic system provides an initial route for cancer cell dissemination in many cancers including melanoma. However, it is largely unknown how the lymphatic system changes during tumor progression due in part to the lack of imaging techniques currently available. In this study, we non-invasively imaged changes of lymphatic function and drainage patterns using near-infrared fluorescence (NIRF) imaging. Dynamic NIRF imaging following intradermal injection of indocyanine green (ICG) was conducted in C57BL/6 mice prior to inoculation of B16F10 murine melanoma cells to the dorsal aspect of the left hindpaw for baseline data or directly to the popliteal lymph node (PLN) and until 21 days post-implantation (p.i.). A series of acquired fluorescent images were quantified to measure lymphatic contractile function. Computed tomography (CT) was also performed to measure the volume of tumor-draining lymph nodes (LNs). We observed significant reduction of lymphatic contractility from 7 days p.i. until 21 days p.i.. Altered lymphatic drainage patterns were also detected at 21 days p.i. in mice with tumor in the paw and at 11 days p.i. in mice with tumor in the PLN, due to lymphatic obstruction of normal lymphatic drainages caused by extensive tumor invasion of draining LNs. Since lymphatic function and architecture were progressively altered during tumor growth and metastasis, non-invasive NIRF imaging may provide a new method to stage disease. In addition, this novel technique can be used as a diagnostic method to non-invasively assess lymphatic response as mechanism of therapeutic action.

No MeSH data available.


Related in: MedlinePlus

Representative CT images (A) of a mouse prior to and at 7, 14, and 21 days p.i. showing a gradually enlarged tumor-draining PLN as shown in red dotted circles. The primary tumor volume on the left paw (B) is significantly increased at 21 days p.i. as compared to that at 14 days p.i.. * p < 0.05 vs. days 7. The volume of tumor-draining PLNs at 21 days p.i. (C) is significantly larger than that in contralateral PLNs. * p < 0.05 vs. contralateral right PLN.
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g005: Representative CT images (A) of a mouse prior to and at 7, 14, and 21 days p.i. showing a gradually enlarged tumor-draining PLN as shown in red dotted circles. The primary tumor volume on the left paw (B) is significantly increased at 21 days p.i. as compared to that at 14 days p.i.. * p < 0.05 vs. days 7. The volume of tumor-draining PLNs at 21 days p.i. (C) is significantly larger than that in contralateral PLNs. * p < 0.05 vs. contralateral right PLN.

Mentions: Although CT cannot accurately assess the cancer status of enlarged LNs and differentiate LN tumor volume from overall LN volume, CT was conducted to measure the volume of tumor draining LNs. Our CT data showed significantly enlarged tumor-draining PLNs at 21 days p.i. (Figs. 5AFig. 5


Direct visualization of changes of lymphatic function and drainage pathways in lymph node metastasis of B16F10 melanoma using near-infrared fluorescence imaging.

Kwon S, Agollah GD, Wu G, Chan W, Sevick-Muraca EM - Biomed Opt Express (2013)

Representative CT images (A) of a mouse prior to and at 7, 14, and 21 days p.i. showing a gradually enlarged tumor-draining PLN as shown in red dotted circles. The primary tumor volume on the left paw (B) is significantly increased at 21 days p.i. as compared to that at 14 days p.i.. * p < 0.05 vs. days 7. The volume of tumor-draining PLNs at 21 days p.i. (C) is significantly larger than that in contralateral PLNs. * p < 0.05 vs. contralateral right PLN.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3675875&req=5

g005: Representative CT images (A) of a mouse prior to and at 7, 14, and 21 days p.i. showing a gradually enlarged tumor-draining PLN as shown in red dotted circles. The primary tumor volume on the left paw (B) is significantly increased at 21 days p.i. as compared to that at 14 days p.i.. * p < 0.05 vs. days 7. The volume of tumor-draining PLNs at 21 days p.i. (C) is significantly larger than that in contralateral PLNs. * p < 0.05 vs. contralateral right PLN.
Mentions: Although CT cannot accurately assess the cancer status of enlarged LNs and differentiate LN tumor volume from overall LN volume, CT was conducted to measure the volume of tumor draining LNs. Our CT data showed significantly enlarged tumor-draining PLNs at 21 days p.i. (Figs. 5AFig. 5

Bottom Line: We observed significant reduction of lymphatic contractility from 7 days p.i. until 21 days p.i..Since lymphatic function and architecture were progressively altered during tumor growth and metastasis, non-invasive NIRF imaging may provide a new method to stage disease.In addition, this novel technique can be used as a diagnostic method to non-invasively assess lymphatic response as mechanism of therapeutic action.

View Article: PubMed Central - PubMed

Affiliation: Center for Molecular Imaging, The Brown Foundation Institute of Molecular Medicine, The University of Texas Health Science Center, Houston, TX 77030, USA.

ABSTRACT
The lymphatic system provides an initial route for cancer cell dissemination in many cancers including melanoma. However, it is largely unknown how the lymphatic system changes during tumor progression due in part to the lack of imaging techniques currently available. In this study, we non-invasively imaged changes of lymphatic function and drainage patterns using near-infrared fluorescence (NIRF) imaging. Dynamic NIRF imaging following intradermal injection of indocyanine green (ICG) was conducted in C57BL/6 mice prior to inoculation of B16F10 murine melanoma cells to the dorsal aspect of the left hindpaw for baseline data or directly to the popliteal lymph node (PLN) and until 21 days post-implantation (p.i.). A series of acquired fluorescent images were quantified to measure lymphatic contractile function. Computed tomography (CT) was also performed to measure the volume of tumor-draining lymph nodes (LNs). We observed significant reduction of lymphatic contractility from 7 days p.i. until 21 days p.i.. Altered lymphatic drainage patterns were also detected at 21 days p.i. in mice with tumor in the paw and at 11 days p.i. in mice with tumor in the PLN, due to lymphatic obstruction of normal lymphatic drainages caused by extensive tumor invasion of draining LNs. Since lymphatic function and architecture were progressively altered during tumor growth and metastasis, non-invasive NIRF imaging may provide a new method to stage disease. In addition, this novel technique can be used as a diagnostic method to non-invasively assess lymphatic response as mechanism of therapeutic action.

No MeSH data available.


Related in: MedlinePlus