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Digital holographic microscopy of the myelin figure structural dynamics and the effect of thermal gradient.

Fathi N, Moradi AR, Habibi M, Vashaee D, Tayebi L - Biomed Opt Express (2013)

Bottom Line: Myelin figures (MFs) are cylindrical multilamellar lipid tubes that can be found in various healthy and diseased living cells.However, after approximately ten seconds, the thickness stabilizes and does not alter significantly.We further studied the effect of the thermal gradient on the length growth.

View Article: PubMed Central - PubMed

Affiliation: Department of Physics, University of Zanjan, PO Box 45195-313, Zanjan, Iran.

ABSTRACT
Myelin figures (MFs) are cylindrical multilamellar lipid tubes that can be found in various healthy and diseased living cells. Their formation and dynamics involve some of the most mysterious configurations that lipid molecules can adopt under certain conditions. They have been studied with different microscopy methods. Due to the frequent coiling of their structure, the usual methods of microscopy fail to give precise quantitative information about their dynamics. In this paper, we introduced Digital Holographic Microscopy (DHM) as a useful method to calculate the precise dynamical volume, thickness, surface and length of the myelin figures. As an example of DHM imaging of myelin figures, their structure and growth rate in the presence and absence of temperature gradient have been studied in this work. We showed that the thickness of a myelin figure can be changed during the first few seconds. However, after approximately ten seconds, the thickness stabilizes and does not alter significantly. We further studied the effect of the thermal gradient on the length growth. The calculation of the length growth from the measurement of the myelin figure volume shows that the length (L) grows in time (t) as [Formula: see text]at the early stage of the myelin protrusion in both the presence and the absence of the thermal gradient. However, thermal gradient facilitates the growth and increases its rate.

No MeSH data available.


Related in: MedlinePlus

(a) Temperature gradient used in the experiments; (b) Conventional microscopy image of myelin figures recorded 10 sec. after hydration of the parent lipid dry drop. The arrow indicates the direction of the thermal gradient from low to high temperature. As thermal gradient can act as an external force, it facilitates the growth of MFs.
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g002: (a) Temperature gradient used in the experiments; (b) Conventional microscopy image of myelin figures recorded 10 sec. after hydration of the parent lipid dry drop. The arrow indicates the direction of the thermal gradient from low to high temperature. As thermal gradient can act as an external force, it facilitates the growth of MFs.

Mentions: The temperature gradient obtained in our set-up and used in the experiments is shown in Fig. 2(a)Fig. 2


Digital holographic microscopy of the myelin figure structural dynamics and the effect of thermal gradient.

Fathi N, Moradi AR, Habibi M, Vashaee D, Tayebi L - Biomed Opt Express (2013)

(a) Temperature gradient used in the experiments; (b) Conventional microscopy image of myelin figures recorded 10 sec. after hydration of the parent lipid dry drop. The arrow indicates the direction of the thermal gradient from low to high temperature. As thermal gradient can act as an external force, it facilitates the growth of MFs.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3675873&req=5

g002: (a) Temperature gradient used in the experiments; (b) Conventional microscopy image of myelin figures recorded 10 sec. after hydration of the parent lipid dry drop. The arrow indicates the direction of the thermal gradient from low to high temperature. As thermal gradient can act as an external force, it facilitates the growth of MFs.
Mentions: The temperature gradient obtained in our set-up and used in the experiments is shown in Fig. 2(a)Fig. 2

Bottom Line: Myelin figures (MFs) are cylindrical multilamellar lipid tubes that can be found in various healthy and diseased living cells.However, after approximately ten seconds, the thickness stabilizes and does not alter significantly.We further studied the effect of the thermal gradient on the length growth.

View Article: PubMed Central - PubMed

Affiliation: Department of Physics, University of Zanjan, PO Box 45195-313, Zanjan, Iran.

ABSTRACT
Myelin figures (MFs) are cylindrical multilamellar lipid tubes that can be found in various healthy and diseased living cells. Their formation and dynamics involve some of the most mysterious configurations that lipid molecules can adopt under certain conditions. They have been studied with different microscopy methods. Due to the frequent coiling of their structure, the usual methods of microscopy fail to give precise quantitative information about their dynamics. In this paper, we introduced Digital Holographic Microscopy (DHM) as a useful method to calculate the precise dynamical volume, thickness, surface and length of the myelin figures. As an example of DHM imaging of myelin figures, their structure and growth rate in the presence and absence of temperature gradient have been studied in this work. We showed that the thickness of a myelin figure can be changed during the first few seconds. However, after approximately ten seconds, the thickness stabilizes and does not alter significantly. We further studied the effect of the thermal gradient on the length growth. The calculation of the length growth from the measurement of the myelin figure volume shows that the length (L) grows in time (t) as [Formula: see text]at the early stage of the myelin protrusion in both the presence and the absence of the thermal gradient. However, thermal gradient facilitates the growth and increases its rate.

No MeSH data available.


Related in: MedlinePlus