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Chiral imaging of collagen by second-harmonic generation circular dichroism.

Lee H, Huttunen MJ, Hsu KJ, Partanen M, Zhuo GY, Kauranen M, Chu SW - Biomed Opt Express (2013)

Bottom Line: Although chirality is an intrinsic structural property of collagen, most of the previous studies ignore that property.We demonstrate chiral imaging of individual collagen fibers by using a laser scanning microscope and type-I collagen from pig ligaments. 100% contrast level of SHG-CD is achieved with sub-micrometer spatial resolution.As a new contrast mechanism for imaging chiral structures in bio-tissues, this technique provides information about collagen morphology and three-dimensional orientation of collagen molecules.

View Article: PubMed Central - PubMed

Affiliation: Department of Physics, National Taiwan University, Taipei 10617, Taiwan.

ABSTRACT
We provide evidence that the chirality of collagen can give rise to strong second-harmonic generation circular dichroism (SHG-CD) responses in nonlinear microscopy. Although chirality is an intrinsic structural property of collagen, most of the previous studies ignore that property. We demonstrate chiral imaging of individual collagen fibers by using a laser scanning microscope and type-I collagen from pig ligaments. 100% contrast level of SHG-CD is achieved with sub-micrometer spatial resolution. As a new contrast mechanism for imaging chiral structures in bio-tissues, this technique provides information about collagen morphology and three-dimensional orientation of collagen molecules.

No MeSH data available.


Optical sections of SHG-CD in a collagen fiber (Media 1).
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Related In: Results  -  Collection


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g003: Optical sections of SHG-CD in a collagen fiber (Media 1).

Mentions: One major advantage of SHG microscopy is its intrinsic optical sectioning capability. With our setup, both the lateral and the axial resolutions are less than 1-μm, as shown in Fig. 3Fig. 3


Chiral imaging of collagen by second-harmonic generation circular dichroism.

Lee H, Huttunen MJ, Hsu KJ, Partanen M, Zhuo GY, Kauranen M, Chu SW - Biomed Opt Express (2013)

Optical sections of SHG-CD in a collagen fiber (Media 1).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3675869&req=5

g003: Optical sections of SHG-CD in a collagen fiber (Media 1).
Mentions: One major advantage of SHG microscopy is its intrinsic optical sectioning capability. With our setup, both the lateral and the axial resolutions are less than 1-μm, as shown in Fig. 3Fig. 3

Bottom Line: Although chirality is an intrinsic structural property of collagen, most of the previous studies ignore that property.We demonstrate chiral imaging of individual collagen fibers by using a laser scanning microscope and type-I collagen from pig ligaments. 100% contrast level of SHG-CD is achieved with sub-micrometer spatial resolution.As a new contrast mechanism for imaging chiral structures in bio-tissues, this technique provides information about collagen morphology and three-dimensional orientation of collagen molecules.

View Article: PubMed Central - PubMed

Affiliation: Department of Physics, National Taiwan University, Taipei 10617, Taiwan.

ABSTRACT
We provide evidence that the chirality of collagen can give rise to strong second-harmonic generation circular dichroism (SHG-CD) responses in nonlinear microscopy. Although chirality is an intrinsic structural property of collagen, most of the previous studies ignore that property. We demonstrate chiral imaging of individual collagen fibers by using a laser scanning microscope and type-I collagen from pig ligaments. 100% contrast level of SHG-CD is achieved with sub-micrometer spatial resolution. As a new contrast mechanism for imaging chiral structures in bio-tissues, this technique provides information about collagen morphology and three-dimensional orientation of collagen molecules.

No MeSH data available.