Expression of zinc and cadmium responsive genes in leaves of willow (Salix caprea L.) genotypes with different accumulation characteristics.
Bottom Line: Salix caprea is well suited for phytoextraction strategies.In a previous survey we showed that genetically distinct S. caprea plants isolated from metal-polluted and unpolluted sites differed in their zinc (Zn) and cadmium (Cd) tolerance and accumulation abilities.These data demonstrate that naturally adapted woody non-model species can help to discover potential novel molecular mechanisms for metal accumulation and tolerance.
Affiliation: Department of Applied Genetics and Cell Biology, BOKU - University of Natural Resources and Life Sciences, Muthgasse 18, A-1190 Vienna, Austria.Show MeSH
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Mentions: The expression of ScMTP1, ScPCS1 and ScPCS2 were below the detection limit (data not shown). All other S. caprea orthologs of known metal responsive genes were expressed in a genotype-specific manner. According to their expression patterns during a combined Zn and Cd treatment of three months they could be grouped into three categories. The first group included the metallothionein genes, ScMT2B and ScMT3, which were induced under metal stress independent of the genotype's origin and metal uptake capacity (Fig. 1). ScMT2B and ScMT3 were highly induced already after two weeks in the metallicolous genotype KH21, whereas the ScMT2B induction was delayed in F20. The second group contained ScSAT1 which was constitutively higher expressed in the metallicolous genotype throughout the whole experiment and additionally strongly induced at the two week time point of metal treatment (Fig. 2). The third group comprised genes that were inducible only in the metallicolous genotype KH21 and not induced or even downregulated in the non-metallicolous genotype F20 (Fig. 3).
Affiliation: Department of Applied Genetics and Cell Biology, BOKU - University of Natural Resources and Life Sciences, Muthgasse 18, A-1190 Vienna, Austria.