Limits...
Expression of zinc and cadmium responsive genes in leaves of willow (Salix caprea L.) genotypes with different accumulation characteristics.

Konlechner C, Türktaş M, Langer I, Vaculík M, Wenzel WW, Puschenreiter M, Hauser MT - Environ. Pollut. (2013)

Bottom Line: Salix caprea is well suited for phytoextraction strategies.In a previous survey we showed that genetically distinct S. caprea plants isolated from metal-polluted and unpolluted sites differed in their zinc (Zn) and cadmium (Cd) tolerance and accumulation abilities.These data demonstrate that naturally adapted woody non-model species can help to discover potential novel molecular mechanisms for metal accumulation and tolerance.

View Article: PubMed Central - PubMed

Affiliation: Department of Applied Genetics and Cell Biology, BOKU - University of Natural Resources and Life Sciences, Muthgasse 18, A-1190 Vienna, Austria.

Show MeSH

Related in: MedlinePlus

Glutathione biosynthesis and transporter genes are only induced after Zn + Cd treatment in leaves of the metallicolous genotype and thus display category (3) expression pattern. qPCR analysis of genotypes KH21 (full bars) and F20 (striped bars) after 2 weeks in perlite cultures without (control, gray) and with 5 mg Zn L−1 and 0.5 mg Cd L−1 (Zn + Cd, black). Expression levels of the glutathione biosynthesis genes, ScOASA1 and ScCAD2, and the transporter genes, ScZIP6 and ScHMA1, were normalized to ScACTIN2. Measurements were carried out in triplicates and values are means ± SE of four biological replicates. Note that ScZIP6 is constitutively higher expressed in F20 in control condition and downregulated to the level of KH21 upon Zn + Cd exposure. Different letters represent significant differences (Student's t-test, p < 0.05).
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3675671&req=5

fig3: Glutathione biosynthesis and transporter genes are only induced after Zn + Cd treatment in leaves of the metallicolous genotype and thus display category (3) expression pattern. qPCR analysis of genotypes KH21 (full bars) and F20 (striped bars) after 2 weeks in perlite cultures without (control, gray) and with 5 mg Zn L−1 and 0.5 mg Cd L−1 (Zn + Cd, black). Expression levels of the glutathione biosynthesis genes, ScOASA1 and ScCAD2, and the transporter genes, ScZIP6 and ScHMA1, were normalized to ScACTIN2. Measurements were carried out in triplicates and values are means ± SE of four biological replicates. Note that ScZIP6 is constitutively higher expressed in F20 in control condition and downregulated to the level of KH21 upon Zn + Cd exposure. Different letters represent significant differences (Student's t-test, p < 0.05).

Mentions: The expression of ScMTP1, ScPCS1 and ScPCS2 were below the detection limit (data not shown). All other S. caprea orthologs of known metal responsive genes were expressed in a genotype-specific manner. According to their expression patterns during a combined Zn and Cd treatment of three months they could be grouped into three categories. The first group included the metallothionein genes, ScMT2B and ScMT3, which were induced under metal stress independent of the genotype's origin and metal uptake capacity (Fig. 1). ScMT2B and ScMT3 were highly induced already after two weeks in the metallicolous genotype KH21, whereas the ScMT2B induction was delayed in F20. The second group contained ScSAT1 which was constitutively higher expressed in the metallicolous genotype throughout the whole experiment and additionally strongly induced at the two week time point of metal treatment (Fig. 2). The third group comprised genes that were inducible only in the metallicolous genotype KH21 and not induced or even downregulated in the non-metallicolous genotype F20 (Fig. 3).


Expression of zinc and cadmium responsive genes in leaves of willow (Salix caprea L.) genotypes with different accumulation characteristics.

Konlechner C, Türktaş M, Langer I, Vaculík M, Wenzel WW, Puschenreiter M, Hauser MT - Environ. Pollut. (2013)

Glutathione biosynthesis and transporter genes are only induced after Zn + Cd treatment in leaves of the metallicolous genotype and thus display category (3) expression pattern. qPCR analysis of genotypes KH21 (full bars) and F20 (striped bars) after 2 weeks in perlite cultures without (control, gray) and with 5 mg Zn L−1 and 0.5 mg Cd L−1 (Zn + Cd, black). Expression levels of the glutathione biosynthesis genes, ScOASA1 and ScCAD2, and the transporter genes, ScZIP6 and ScHMA1, were normalized to ScACTIN2. Measurements were carried out in triplicates and values are means ± SE of four biological replicates. Note that ScZIP6 is constitutively higher expressed in F20 in control condition and downregulated to the level of KH21 upon Zn + Cd exposure. Different letters represent significant differences (Student's t-test, p < 0.05).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3675671&req=5

fig3: Glutathione biosynthesis and transporter genes are only induced after Zn + Cd treatment in leaves of the metallicolous genotype and thus display category (3) expression pattern. qPCR analysis of genotypes KH21 (full bars) and F20 (striped bars) after 2 weeks in perlite cultures without (control, gray) and with 5 mg Zn L−1 and 0.5 mg Cd L−1 (Zn + Cd, black). Expression levels of the glutathione biosynthesis genes, ScOASA1 and ScCAD2, and the transporter genes, ScZIP6 and ScHMA1, were normalized to ScACTIN2. Measurements were carried out in triplicates and values are means ± SE of four biological replicates. Note that ScZIP6 is constitutively higher expressed in F20 in control condition and downregulated to the level of KH21 upon Zn + Cd exposure. Different letters represent significant differences (Student's t-test, p < 0.05).
Mentions: The expression of ScMTP1, ScPCS1 and ScPCS2 were below the detection limit (data not shown). All other S. caprea orthologs of known metal responsive genes were expressed in a genotype-specific manner. According to their expression patterns during a combined Zn and Cd treatment of three months they could be grouped into three categories. The first group included the metallothionein genes, ScMT2B and ScMT3, which were induced under metal stress independent of the genotype's origin and metal uptake capacity (Fig. 1). ScMT2B and ScMT3 were highly induced already after two weeks in the metallicolous genotype KH21, whereas the ScMT2B induction was delayed in F20. The second group contained ScSAT1 which was constitutively higher expressed in the metallicolous genotype throughout the whole experiment and additionally strongly induced at the two week time point of metal treatment (Fig. 2). The third group comprised genes that were inducible only in the metallicolous genotype KH21 and not induced or even downregulated in the non-metallicolous genotype F20 (Fig. 3).

Bottom Line: Salix caprea is well suited for phytoextraction strategies.In a previous survey we showed that genetically distinct S. caprea plants isolated from metal-polluted and unpolluted sites differed in their zinc (Zn) and cadmium (Cd) tolerance and accumulation abilities.These data demonstrate that naturally adapted woody non-model species can help to discover potential novel molecular mechanisms for metal accumulation and tolerance.

View Article: PubMed Central - PubMed

Affiliation: Department of Applied Genetics and Cell Biology, BOKU - University of Natural Resources and Life Sciences, Muthgasse 18, A-1190 Vienna, Austria.

Show MeSH
Related in: MedlinePlus