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Fingolimod--a sphingosine-like molecule inhibits vesicle mobility and secretion in astrocytes.

Trkov S, Stenovec M, Kreft M, Potokar M, Parpura V, Davletov B, Zorec R - Glia (2012)

Bottom Line: In the brain, astrocytes signal to the neighboring cells by the release of chemical messengers (gliotransmitters) via regulated exocytosis.Recent studies uncovered a potential role of signaling lipids in modulation of exocytosis.We conclude that in astrocytes, cell permeable sphingosine-like lipids affect regulated exocytosis by attenuating vesicle mobility, thereby preventing effective vesicle access/interaction with the plasma membrane docking/release sites.

View Article: PubMed Central - PubMed

Affiliation: Celica d.o.o., Biomedical Center, Technology Park 24, Ljubljana, Slovenia.

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Related in: MedlinePlus

The mobility of astrocytic peptidergic vesicles is reduced by FTY720. (A) Live cultured astrocyte under DIC optics and (B) the confocal image of the same cell transfected to express ANP.emd that is stored inside peptidergic vesicles observed as numerous green fluorescent puncta; scale bars, 20 μm. (C) Vesicle tracks (n = 40) obtained in nontreated control astrocyte (Cont) and in (D) another astrocyte treated with 20 μM FTY720. Many elongated vesicles tracks in the control cell indicate substantial vesicle mobility. In the cell treated with 20 μM FTY720, the mobility of vesicles was attenuated; note the absence of elongated vesicle tracks. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]
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fig01: The mobility of astrocytic peptidergic vesicles is reduced by FTY720. (A) Live cultured astrocyte under DIC optics and (B) the confocal image of the same cell transfected to express ANP.emd that is stored inside peptidergic vesicles observed as numerous green fluorescent puncta; scale bars, 20 μm. (C) Vesicle tracks (n = 40) obtained in nontreated control astrocyte (Cont) and in (D) another astrocyte treated with 20 μM FTY720. Many elongated vesicles tracks in the control cell indicate substantial vesicle mobility. In the cell treated with 20 μM FTY720, the mobility of vesicles was attenuated; note the absence of elongated vesicle tracks. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]

Mentions: To investigate the effect of FTY720 treatment on vesicle mobility, the fluorescently labeled peptidergic vesicles preloaded with ANP.emd (Han et al.,1999; Krzan et al.,2003) were monitored in cultured astrocytes (Fig. 1A) by confocal microscopy and the motion patterns of individual vesicles (Fig. 1B) analyzed. The vesicle pathways (tracks) obtained in nontreated cells were elongated (Fig. 1C), indicating substantial mobility, while in FTY720 treated cells vesicle tracks were highly contorted (Fig. 1D), indicating strongly attenuated vesicle mobility.


Fingolimod--a sphingosine-like molecule inhibits vesicle mobility and secretion in astrocytes.

Trkov S, Stenovec M, Kreft M, Potokar M, Parpura V, Davletov B, Zorec R - Glia (2012)

The mobility of astrocytic peptidergic vesicles is reduced by FTY720. (A) Live cultured astrocyte under DIC optics and (B) the confocal image of the same cell transfected to express ANP.emd that is stored inside peptidergic vesicles observed as numerous green fluorescent puncta; scale bars, 20 μm. (C) Vesicle tracks (n = 40) obtained in nontreated control astrocyte (Cont) and in (D) another astrocyte treated with 20 μM FTY720. Many elongated vesicles tracks in the control cell indicate substantial vesicle mobility. In the cell treated with 20 μM FTY720, the mobility of vesicles was attenuated; note the absence of elongated vesicle tracks. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3675637&req=5

fig01: The mobility of astrocytic peptidergic vesicles is reduced by FTY720. (A) Live cultured astrocyte under DIC optics and (B) the confocal image of the same cell transfected to express ANP.emd that is stored inside peptidergic vesicles observed as numerous green fluorescent puncta; scale bars, 20 μm. (C) Vesicle tracks (n = 40) obtained in nontreated control astrocyte (Cont) and in (D) another astrocyte treated with 20 μM FTY720. Many elongated vesicles tracks in the control cell indicate substantial vesicle mobility. In the cell treated with 20 μM FTY720, the mobility of vesicles was attenuated; note the absence of elongated vesicle tracks. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]
Mentions: To investigate the effect of FTY720 treatment on vesicle mobility, the fluorescently labeled peptidergic vesicles preloaded with ANP.emd (Han et al.,1999; Krzan et al.,2003) were monitored in cultured astrocytes (Fig. 1A) by confocal microscopy and the motion patterns of individual vesicles (Fig. 1B) analyzed. The vesicle pathways (tracks) obtained in nontreated cells were elongated (Fig. 1C), indicating substantial mobility, while in FTY720 treated cells vesicle tracks were highly contorted (Fig. 1D), indicating strongly attenuated vesicle mobility.

Bottom Line: In the brain, astrocytes signal to the neighboring cells by the release of chemical messengers (gliotransmitters) via regulated exocytosis.Recent studies uncovered a potential role of signaling lipids in modulation of exocytosis.We conclude that in astrocytes, cell permeable sphingosine-like lipids affect regulated exocytosis by attenuating vesicle mobility, thereby preventing effective vesicle access/interaction with the plasma membrane docking/release sites.

View Article: PubMed Central - PubMed

Affiliation: Celica d.o.o., Biomedical Center, Technology Park 24, Ljubljana, Slovenia.

Show MeSH
Related in: MedlinePlus