Fingolimod--a sphingosine-like molecule inhibits vesicle mobility and secretion in astrocytes.
Bottom Line: In the brain, astrocytes signal to the neighboring cells by the release of chemical messengers (gliotransmitters) via regulated exocytosis.Recent studies uncovered a potential role of signaling lipids in modulation of exocytosis.We conclude that in astrocytes, cell permeable sphingosine-like lipids affect regulated exocytosis by attenuating vesicle mobility, thereby preventing effective vesicle access/interaction with the plasma membrane docking/release sites.
Affiliation: Celica d.o.o., Biomedical Center, Technology Park 24, Ljubljana, Slovenia.Show MeSH
Related in: MedlinePlus
Mentions: To investigate the effect of FTY720 treatment on vesicle mobility, the fluorescently labeled peptidergic vesicles preloaded with ANP.emd (Han et al.,1999; Krzan et al.,2003) were monitored in cultured astrocytes (Fig. 1A) by confocal microscopy and the motion patterns of individual vesicles (Fig. 1B) analyzed. The vesicle pathways (tracks) obtained in nontreated cells were elongated (Fig. 1C), indicating substantial mobility, while in FTY720 treated cells vesicle tracks were highly contorted (Fig. 1D), indicating strongly attenuated vesicle mobility.
Affiliation: Celica d.o.o., Biomedical Center, Technology Park 24, Ljubljana, Slovenia.