Limits...
Clinical presentation and sequence analyses of HA and NA antigens of the novel H7N9 viruses.

Han J, Niu F, Jin M, Wang L, Liu J, Zhang P, Xie B, Wu X, Wen D, Ji L, Liu G, Yang Z, Zhang C, Zhou D, Leng Q, Lan K - Emerg Microbes Infect (2013)

Bottom Line: The patient had been exposed to poultry before disease onset.Phylogenetic analyses of hemagglutinin and neuraminidase genes showed a close genetic relationship between viruses from the patient and from poultry booths where he had visited, indicating that the patient may have been exposed from the infected poultry.Furthermore, we found five novel H7N9 virus-specific sequence variations in receptor-binding site of hemagglutinin, which may be associated with the acquisition of the ability to infect humans.

View Article: PubMed Central - PubMed

Affiliation: Huzhou Center for Disease Control and Prevention , Huzhou 313000, Zhejiang, China.

ABSTRACT
Recently, a novel H7N9 avian influenza A virus has led to a human influenza outbreak in China. Here we report a 64-year old man with possible history of chronic bronchitis died from the H7N9 infection in Huzhou City, Zhejiang Province in Eastern China. The patient had been exposed to poultry before disease onset. Phylogenetic analyses of hemagglutinin and neuraminidase genes showed a close genetic relationship between viruses from the patient and from poultry booths where he had visited, indicating that the patient may have been exposed from the infected poultry. Two poultry venders and close contacts of the patient were negative for H7N9, suggesting that there are some unknown mechanisms to prevent them from being infected by the novel H7N9 virus. Furthermore, we found five novel H7N9 virus-specific sequence variations in receptor-binding site of hemagglutinin, which may be associated with the acquisition of the ability to infect humans.

No MeSH data available.


Related in: MedlinePlus

Phylogenetic tree of influenza HA (A) and NA (B) sequences. The phylogenetic trees were constructed with MEGA 5.0 using the neighbor-joining method. The stability of the nodes was assessed by bootstrap analysis with 1000 replications, and only bootstrap values of ≥80 were shown at the corresponding nodes. The sequences from this patient and the poultry in Huzhou are highlighted by red circle and triangles, respectively. The blue diamonds indicate the sequences of A/H7N9 viruses from other regions of China.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3675404&req=5

fig2: Phylogenetic tree of influenza HA (A) and NA (B) sequences. The phylogenetic trees were constructed with MEGA 5.0 using the neighbor-joining method. The stability of the nodes was assessed by bootstrap analysis with 1000 replications, and only bootstrap values of ≥80 were shown at the corresponding nodes. The sequences from this patient and the poultry in Huzhou are highlighted by red circle and triangles, respectively. The blue diamonds indicate the sequences of A/H7N9 viruses from other regions of China.

Mentions: Phylogenetic trees of HA and NA fragments showed that the novel H7N9 strains form a well-supported clade (with a bootstrap value of 100%) and the sequence from this patient clusters closely with those from the patients in Shanghai, Auhui and Zhejiang, as well as those from the poultry (including chickens and pigeon) from the local area and Shanghai (Figure 2). These results not only support that this patient was infected by the novel H7N9 virus, but also suggest that the novel H7N9 virus was present in the environment where the patient was exposed.


Clinical presentation and sequence analyses of HA and NA antigens of the novel H7N9 viruses.

Han J, Niu F, Jin M, Wang L, Liu J, Zhang P, Xie B, Wu X, Wen D, Ji L, Liu G, Yang Z, Zhang C, Zhou D, Leng Q, Lan K - Emerg Microbes Infect (2013)

Phylogenetic tree of influenza HA (A) and NA (B) sequences. The phylogenetic trees were constructed with MEGA 5.0 using the neighbor-joining method. The stability of the nodes was assessed by bootstrap analysis with 1000 replications, and only bootstrap values of ≥80 were shown at the corresponding nodes. The sequences from this patient and the poultry in Huzhou are highlighted by red circle and triangles, respectively. The blue diamonds indicate the sequences of A/H7N9 viruses from other regions of China.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3675404&req=5

fig2: Phylogenetic tree of influenza HA (A) and NA (B) sequences. The phylogenetic trees were constructed with MEGA 5.0 using the neighbor-joining method. The stability of the nodes was assessed by bootstrap analysis with 1000 replications, and only bootstrap values of ≥80 were shown at the corresponding nodes. The sequences from this patient and the poultry in Huzhou are highlighted by red circle and triangles, respectively. The blue diamonds indicate the sequences of A/H7N9 viruses from other regions of China.
Mentions: Phylogenetic trees of HA and NA fragments showed that the novel H7N9 strains form a well-supported clade (with a bootstrap value of 100%) and the sequence from this patient clusters closely with those from the patients in Shanghai, Auhui and Zhejiang, as well as those from the poultry (including chickens and pigeon) from the local area and Shanghai (Figure 2). These results not only support that this patient was infected by the novel H7N9 virus, but also suggest that the novel H7N9 virus was present in the environment where the patient was exposed.

Bottom Line: The patient had been exposed to poultry before disease onset.Phylogenetic analyses of hemagglutinin and neuraminidase genes showed a close genetic relationship between viruses from the patient and from poultry booths where he had visited, indicating that the patient may have been exposed from the infected poultry.Furthermore, we found five novel H7N9 virus-specific sequence variations in receptor-binding site of hemagglutinin, which may be associated with the acquisition of the ability to infect humans.

View Article: PubMed Central - PubMed

Affiliation: Huzhou Center for Disease Control and Prevention , Huzhou 313000, Zhejiang, China.

ABSTRACT
Recently, a novel H7N9 avian influenza A virus has led to a human influenza outbreak in China. Here we report a 64-year old man with possible history of chronic bronchitis died from the H7N9 infection in Huzhou City, Zhejiang Province in Eastern China. The patient had been exposed to poultry before disease onset. Phylogenetic analyses of hemagglutinin and neuraminidase genes showed a close genetic relationship between viruses from the patient and from poultry booths where he had visited, indicating that the patient may have been exposed from the infected poultry. Two poultry venders and close contacts of the patient were negative for H7N9, suggesting that there are some unknown mechanisms to prevent them from being infected by the novel H7N9 virus. Furthermore, we found five novel H7N9 virus-specific sequence variations in receptor-binding site of hemagglutinin, which may be associated with the acquisition of the ability to infect humans.

No MeSH data available.


Related in: MedlinePlus