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Effect of biofilm formation, and biocorrosion on denture base fractures.

Sahin C, Ergin A, Ayyildiz S, Cosgun E, Uzun G - J Adv Prosthodont (2013)

Bottom Line: The aim of this study was to investigate the destructive effects of biofilm formation and/or biocorrosive activity of 6 different oral microorganisms.According to normality assumptions ANOVA or Kruskal-Wallis was selected for statistical analysis (α=0.05).Significant differences were obtained among the adhesion potential of 6 different microorganisms and there were significant differences among their adhesion onto 3 different denture base materials.

View Article: PubMed Central - PubMed

Affiliation: School of Health Services, Dental Prosthetics Technology, Hacettepe University, Ankara, Turkey.

ABSTRACT

Purpose: The aim of this study was to investigate the destructive effects of biofilm formation and/or biocorrosive activity of 6 different oral microorganisms.

Materials and methods: Three different heat polymerized acrylic resins (Ivocap Plus, Lucitone 550, QC 20) were used to prepare three different types of samples. Type "A" samples with "V" type notch was used to measure the fracture strength, "B" type to evaluate the surfaces with scanning electron microscopy and "C" type for quantitative biofilm assay. Development and calculation of biofilm covered surfaces on denture base materials were accomplished by SEM and quantitative biofilm assay. According to normality assumptions ANOVA or Kruskal-Wallis was selected for statistical analysis (α=0.05).

Results: Significant differences were obtained among the adhesion potential of 6 different microorganisms and there were significant differences among their adhesion onto 3 different denture base materials. Compared to the control groups after contamination with the microorganisms, the three point bending test values of denture base materials decreased significantly (P<.05); microorganisms diffused at least 52% of the denture base surface. The highest median quantitative biofilm value within all the denture base materials was obtained with P. aeruginosa on Lucitone 550. The type of denture base material did not alter the diffusion potential of the microorganisms significantly (P>.05).

Conclusion: All the tested microorganisms had destructive effect over the structure and composition of the denture base materials.

No MeSH data available.


Related in: MedlinePlus

Schematic view of 'V' type notch carving.
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Related In: Results  -  Collection

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Figure 1: Schematic view of 'V' type notch carving.

Mentions: Three different heat polymerized acrylic resins (Table 1) were used to prepare 50 × 15 × 4 mm (Type A; for three point bending (TPB) test, n=210), 8 × 8 × 1 mm (Type B; for SEM analysis, n=54) and 2 × 2 × 2 mm (Type C; for spectrophotometer analysis, n=180) samples (Table 2). Negative molds of the metal masters were obtained with a medium viscosity impression material. This technique was used to fabricate all types of the specimens. Wax patterns were invested in metal dental flasks. Acrylic resins were polymerized according to manufacturers' instructions. Flasks were left for 180 minutes cooling. Next, each specimen were deflasked and finished with 320, 400 and 600-grit silicone carbid papers. To simulate a crack line on the denture base, "V" type notch was carved in the middle of each specimen of impact test groups (A type) along with the 15 mm surface by using a milling machine and a milling tool as shown in the Fig. 1. The depth was 2 ± 0.2 mm. All type of specimens were ultrasonically cleaned for 20 minutes and immersed in distilled water for 48 hours at 37℃ before tests.


Effect of biofilm formation, and biocorrosion on denture base fractures.

Sahin C, Ergin A, Ayyildiz S, Cosgun E, Uzun G - J Adv Prosthodont (2013)

Schematic view of 'V' type notch carving.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3675286&req=5

Figure 1: Schematic view of 'V' type notch carving.
Mentions: Three different heat polymerized acrylic resins (Table 1) were used to prepare 50 × 15 × 4 mm (Type A; for three point bending (TPB) test, n=210), 8 × 8 × 1 mm (Type B; for SEM analysis, n=54) and 2 × 2 × 2 mm (Type C; for spectrophotometer analysis, n=180) samples (Table 2). Negative molds of the metal masters were obtained with a medium viscosity impression material. This technique was used to fabricate all types of the specimens. Wax patterns were invested in metal dental flasks. Acrylic resins were polymerized according to manufacturers' instructions. Flasks were left for 180 minutes cooling. Next, each specimen were deflasked and finished with 320, 400 and 600-grit silicone carbid papers. To simulate a crack line on the denture base, "V" type notch was carved in the middle of each specimen of impact test groups (A type) along with the 15 mm surface by using a milling machine and a milling tool as shown in the Fig. 1. The depth was 2 ± 0.2 mm. All type of specimens were ultrasonically cleaned for 20 minutes and immersed in distilled water for 48 hours at 37℃ before tests.

Bottom Line: The aim of this study was to investigate the destructive effects of biofilm formation and/or biocorrosive activity of 6 different oral microorganisms.According to normality assumptions ANOVA or Kruskal-Wallis was selected for statistical analysis (α=0.05).Significant differences were obtained among the adhesion potential of 6 different microorganisms and there were significant differences among their adhesion onto 3 different denture base materials.

View Article: PubMed Central - PubMed

Affiliation: School of Health Services, Dental Prosthetics Technology, Hacettepe University, Ankara, Turkey.

ABSTRACT

Purpose: The aim of this study was to investigate the destructive effects of biofilm formation and/or biocorrosive activity of 6 different oral microorganisms.

Materials and methods: Three different heat polymerized acrylic resins (Ivocap Plus, Lucitone 550, QC 20) were used to prepare three different types of samples. Type "A" samples with "V" type notch was used to measure the fracture strength, "B" type to evaluate the surfaces with scanning electron microscopy and "C" type for quantitative biofilm assay. Development and calculation of biofilm covered surfaces on denture base materials were accomplished by SEM and quantitative biofilm assay. According to normality assumptions ANOVA or Kruskal-Wallis was selected for statistical analysis (α=0.05).

Results: Significant differences were obtained among the adhesion potential of 6 different microorganisms and there were significant differences among their adhesion onto 3 different denture base materials. Compared to the control groups after contamination with the microorganisms, the three point bending test values of denture base materials decreased significantly (P<.05); microorganisms diffused at least 52% of the denture base surface. The highest median quantitative biofilm value within all the denture base materials was obtained with P. aeruginosa on Lucitone 550. The type of denture base material did not alter the diffusion potential of the microorganisms significantly (P>.05).

Conclusion: All the tested microorganisms had destructive effect over the structure and composition of the denture base materials.

No MeSH data available.


Related in: MedlinePlus