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Global expression profiling of transcription factor genes provides new insights into pathogenicity and stress responses in the rice blast fungus.

Park SY, Choi J, Lim SE, Lee GW, Park J, Kim Y, Kong S, Kim SR, Rho HS, Jeon J, Chi MH, Kim S, Khang CH, Kang S, Lee YH - PLoS Pathog. (2013)

Bottom Line: Mutants of in planta inducible genes not only exhibited sensitive to oxidative stress but also failed to infect rice.These experimental validations clearly demonstrated the value of TF expression patterns in predicting the function of individual TF genes.The regulatory network of TF genes revealed by this study provides a solid foundation for elucidating how M. oryzae regulates its pathogenesis, development, and stress responses.

View Article: PubMed Central - PubMed

Affiliation: Department of Agricultural Biotechnology, Fungal Bioinformatics Laboratory, Center for Fungal Genetic Resources, and Center for Fungal Pathogenesis, Seoul National University, Seoul, Korea.

ABSTRACT
Because most efforts to understand the molecular mechanisms underpinning fungal pathogenicity have focused on studying the function and role of individual genes, relatively little is known about how transcriptional machineries globally regulate and coordinate the expression of a large group of genes involved in pathogenesis. Using quantitative real-time PCR, we analyzed the expression patterns of 206 transcription factor (TF) genes in the rice blast fungus Magnaporthe oryzae under 32 conditions, including multiple infection-related developmental stages and various abiotic stresses. The resulting data, which are publicly available via an online platform, provided new insights into how these TFs are regulated and potentially work together to control cellular responses to a diverse array of stimuli. High degrees of differential TF expression were observed under the conditions tested. More than 50% of the 206 TF genes were up-regulated during conidiation and/or in conidia. Mutations in ten conidiation-specific TF genes caused defects in conidiation. Expression patterns in planta were similar to those under oxidative stress conditions. Mutants of in planta inducible genes not only exhibited sensitive to oxidative stress but also failed to infect rice. These experimental validations clearly demonstrated the value of TF expression patterns in predicting the function of individual TF genes. The regulatory network of TF genes revealed by this study provides a solid foundation for elucidating how M. oryzae regulates its pathogenesis, development, and stress responses.

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Expression profiles of 57 conidiation-specific TF genes in six TF gene deletion mutants.The mutants included ΔMoaps1, ΔMoaps2, ΔMohox2, ΔMohox4, ΔMoleu3, and ΔMonit4. Up-regulated genes in the mutants (more than 2 fold) are indicated by red bars, and down-regulated genes (less than 0.5 fold) are noted by blue bars. The genes that did not show differential expression in the six mutants are marked in blue.
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ppat-1003350-g006: Expression profiles of 57 conidiation-specific TF genes in six TF gene deletion mutants.The mutants included ΔMoaps1, ΔMoaps2, ΔMohox2, ΔMohox4, ΔMoleu3, and ΔMonit4. Up-regulated genes in the mutants (more than 2 fold) are indicated by red bars, and down-regulated genes (less than 0.5 fold) are noted by blue bars. The genes that did not show differential expression in the six mutants are marked in blue.

Mentions: To investigate the regulatory network controlling the expression and interactions of these 57 genes during conidiation and/or in conidia, we examined their expression in six TF gene deletion mutants. These mutants showed conidiation-related phenotypes such as no conidial production (ΔMohox2[12]), smaller conidia (ΔMohox4[12]), and reduced conidial production (ΔMoaps1(this study), ΔMoaps2 (this study), ΔMoleu3[48], and ΔMonit4[48]). We compared gene expression profiles of these 57 genes in the six mutants with those in KJ201 to determine if and how their gene expression was affected by each mutation (Figure 6). Sixteen genes (Figure 6) were not affected by any of the mutations.


Global expression profiling of transcription factor genes provides new insights into pathogenicity and stress responses in the rice blast fungus.

Park SY, Choi J, Lim SE, Lee GW, Park J, Kim Y, Kong S, Kim SR, Rho HS, Jeon J, Chi MH, Kim S, Khang CH, Kang S, Lee YH - PLoS Pathog. (2013)

Expression profiles of 57 conidiation-specific TF genes in six TF gene deletion mutants.The mutants included ΔMoaps1, ΔMoaps2, ΔMohox2, ΔMohox4, ΔMoleu3, and ΔMonit4. Up-regulated genes in the mutants (more than 2 fold) are indicated by red bars, and down-regulated genes (less than 0.5 fold) are noted by blue bars. The genes that did not show differential expression in the six mutants are marked in blue.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3675110&req=5

ppat-1003350-g006: Expression profiles of 57 conidiation-specific TF genes in six TF gene deletion mutants.The mutants included ΔMoaps1, ΔMoaps2, ΔMohox2, ΔMohox4, ΔMoleu3, and ΔMonit4. Up-regulated genes in the mutants (more than 2 fold) are indicated by red bars, and down-regulated genes (less than 0.5 fold) are noted by blue bars. The genes that did not show differential expression in the six mutants are marked in blue.
Mentions: To investigate the regulatory network controlling the expression and interactions of these 57 genes during conidiation and/or in conidia, we examined their expression in six TF gene deletion mutants. These mutants showed conidiation-related phenotypes such as no conidial production (ΔMohox2[12]), smaller conidia (ΔMohox4[12]), and reduced conidial production (ΔMoaps1(this study), ΔMoaps2 (this study), ΔMoleu3[48], and ΔMonit4[48]). We compared gene expression profiles of these 57 genes in the six mutants with those in KJ201 to determine if and how their gene expression was affected by each mutation (Figure 6). Sixteen genes (Figure 6) were not affected by any of the mutations.

Bottom Line: Mutants of in planta inducible genes not only exhibited sensitive to oxidative stress but also failed to infect rice.These experimental validations clearly demonstrated the value of TF expression patterns in predicting the function of individual TF genes.The regulatory network of TF genes revealed by this study provides a solid foundation for elucidating how M. oryzae regulates its pathogenesis, development, and stress responses.

View Article: PubMed Central - PubMed

Affiliation: Department of Agricultural Biotechnology, Fungal Bioinformatics Laboratory, Center for Fungal Genetic Resources, and Center for Fungal Pathogenesis, Seoul National University, Seoul, Korea.

ABSTRACT
Because most efforts to understand the molecular mechanisms underpinning fungal pathogenicity have focused on studying the function and role of individual genes, relatively little is known about how transcriptional machineries globally regulate and coordinate the expression of a large group of genes involved in pathogenesis. Using quantitative real-time PCR, we analyzed the expression patterns of 206 transcription factor (TF) genes in the rice blast fungus Magnaporthe oryzae under 32 conditions, including multiple infection-related developmental stages and various abiotic stresses. The resulting data, which are publicly available via an online platform, provided new insights into how these TFs are regulated and potentially work together to control cellular responses to a diverse array of stimuli. High degrees of differential TF expression were observed under the conditions tested. More than 50% of the 206 TF genes were up-regulated during conidiation and/or in conidia. Mutations in ten conidiation-specific TF genes caused defects in conidiation. Expression patterns in planta were similar to those under oxidative stress conditions. Mutants of in planta inducible genes not only exhibited sensitive to oxidative stress but also failed to infect rice. These experimental validations clearly demonstrated the value of TF expression patterns in predicting the function of individual TF genes. The regulatory network of TF genes revealed by this study provides a solid foundation for elucidating how M. oryzae regulates its pathogenesis, development, and stress responses.

Show MeSH
Related in: MedlinePlus