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Antibody responses to Sarcoptes scabiei apolipoprotein in a porcine model: relevance to immunodiagnosis of recent infection.

Rampton M, Walton SF, Holt DC, Pasay C, Kelly A, Currie BJ, McCarthy JS, Mounsey KE - PLoS ONE (2013)

Bottom Line: We utilised a porcine model to prospectively compare specific antibody responses to a primary infestation by ELISA, to Sar s 14.3 and to S. scabiei whole mite antigen extract (WMA).Differences in the antibody profile between antigens were apparent, with Sar s 14.3 responses detected earlier, and declining significantly after peak infestation compared to WMA.Both antigens resulted in >90% diagnostic sensitivity from weeks 8-16 post infestation.

View Article: PubMed Central - PubMed

Affiliation: School of Health and Sport Sciences, University of the Sunshine Coast, Maroochydore, Queensland, Australia.

ABSTRACT
No commercial immunodiagnostic tests for human scabies are currently available, and existing animal tests are not sufficiently sensitive. The recombinant Sarcoptes scabiei apolipoprotein antigen Sar s 14.3 is a promising immunodiagnostic, eliciting high levels of IgE and IgG in infected people. Limited data are available regarding the temporal development of antibodies to Sar s 14.3, an issue of relevance in terms of immunodiagnosis. We utilised a porcine model to prospectively compare specific antibody responses to a primary infestation by ELISA, to Sar s 14.3 and to S. scabiei whole mite antigen extract (WMA). Differences in the antibody profile between antigens were apparent, with Sar s 14.3 responses detected earlier, and declining significantly after peak infestation compared to WMA. Both antigens resulted in >90% diagnostic sensitivity from weeks 8-16 post infestation. These data provide important information on the temporal development of humoral immune responses in scabies and further supports the development of recombinant antigen based immunodiagnostic tests for recent scabies infestations.

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Related in: MedlinePlus

Correlation between lesion severity and antibody response.Scatter plot showing lesion score and ELISA units from mange infected pigs in the non-Dex treatment group from weeks 8–20 post infection. Each point represents the lesion score and antibody level for an individual pig at a single time point. R: Spearmans correlation coefficient.
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pone-0065354-g004: Correlation between lesion severity and antibody response.Scatter plot showing lesion score and ELISA units from mange infected pigs in the non-Dex treatment group from weeks 8–20 post infection. Each point represents the lesion score and antibody level for an individual pig at a single time point. R: Spearmans correlation coefficient.

Mentions: Moderate positive correlations were observed in the anti-WMA, IgA (r = 0.53, p = 0.003) and anti-Sar s 14.3, IgG1 (r = 0.52, p = 0.006) ELISAs. Conversely, a negative correlation was observed in anti-WMA IgG2 ELISA (r = −0.5, p−0.007) (Fig. 4). There was no correlation observed for any other antigen/antibody combinations.


Antibody responses to Sarcoptes scabiei apolipoprotein in a porcine model: relevance to immunodiagnosis of recent infection.

Rampton M, Walton SF, Holt DC, Pasay C, Kelly A, Currie BJ, McCarthy JS, Mounsey KE - PLoS ONE (2013)

Correlation between lesion severity and antibody response.Scatter plot showing lesion score and ELISA units from mange infected pigs in the non-Dex treatment group from weeks 8–20 post infection. Each point represents the lesion score and antibody level for an individual pig at a single time point. R: Spearmans correlation coefficient.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3675102&req=5

pone-0065354-g004: Correlation between lesion severity and antibody response.Scatter plot showing lesion score and ELISA units from mange infected pigs in the non-Dex treatment group from weeks 8–20 post infection. Each point represents the lesion score and antibody level for an individual pig at a single time point. R: Spearmans correlation coefficient.
Mentions: Moderate positive correlations were observed in the anti-WMA, IgA (r = 0.53, p = 0.003) and anti-Sar s 14.3, IgG1 (r = 0.52, p = 0.006) ELISAs. Conversely, a negative correlation was observed in anti-WMA IgG2 ELISA (r = −0.5, p−0.007) (Fig. 4). There was no correlation observed for any other antigen/antibody combinations.

Bottom Line: We utilised a porcine model to prospectively compare specific antibody responses to a primary infestation by ELISA, to Sar s 14.3 and to S. scabiei whole mite antigen extract (WMA).Differences in the antibody profile between antigens were apparent, with Sar s 14.3 responses detected earlier, and declining significantly after peak infestation compared to WMA.Both antigens resulted in >90% diagnostic sensitivity from weeks 8-16 post infestation.

View Article: PubMed Central - PubMed

Affiliation: School of Health and Sport Sciences, University of the Sunshine Coast, Maroochydore, Queensland, Australia.

ABSTRACT
No commercial immunodiagnostic tests for human scabies are currently available, and existing animal tests are not sufficiently sensitive. The recombinant Sarcoptes scabiei apolipoprotein antigen Sar s 14.3 is a promising immunodiagnostic, eliciting high levels of IgE and IgG in infected people. Limited data are available regarding the temporal development of antibodies to Sar s 14.3, an issue of relevance in terms of immunodiagnosis. We utilised a porcine model to prospectively compare specific antibody responses to a primary infestation by ELISA, to Sar s 14.3 and to S. scabiei whole mite antigen extract (WMA). Differences in the antibody profile between antigens were apparent, with Sar s 14.3 responses detected earlier, and declining significantly after peak infestation compared to WMA. Both antigens resulted in >90% diagnostic sensitivity from weeks 8-16 post infestation. These data provide important information on the temporal development of humoral immune responses in scabies and further supports the development of recombinant antigen based immunodiagnostic tests for recent scabies infestations.

Show MeSH
Related in: MedlinePlus