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Antibody responses to Sarcoptes scabiei apolipoprotein in a porcine model: relevance to immunodiagnosis of recent infection.

Rampton M, Walton SF, Holt DC, Pasay C, Kelly A, Currie BJ, McCarthy JS, Mounsey KE - PLoS ONE (2013)

Bottom Line: We utilised a porcine model to prospectively compare specific antibody responses to a primary infestation by ELISA, to Sar s 14.3 and to S. scabiei whole mite antigen extract (WMA).Differences in the antibody profile between antigens were apparent, with Sar s 14.3 responses detected earlier, and declining significantly after peak infestation compared to WMA.Both antigens resulted in >90% diagnostic sensitivity from weeks 8-16 post infestation.

View Article: PubMed Central - PubMed

Affiliation: School of Health and Sport Sciences, University of the Sunshine Coast, Maroochydore, Queensland, Australia.

ABSTRACT
No commercial immunodiagnostic tests for human scabies are currently available, and existing animal tests are not sufficiently sensitive. The recombinant Sarcoptes scabiei apolipoprotein antigen Sar s 14.3 is a promising immunodiagnostic, eliciting high levels of IgE and IgG in infected people. Limited data are available regarding the temporal development of antibodies to Sar s 14.3, an issue of relevance in terms of immunodiagnosis. We utilised a porcine model to prospectively compare specific antibody responses to a primary infestation by ELISA, to Sar s 14.3 and to S. scabiei whole mite antigen extract (WMA). Differences in the antibody profile between antigens were apparent, with Sar s 14.3 responses detected earlier, and declining significantly after peak infestation compared to WMA. Both antigens resulted in >90% diagnostic sensitivity from weeks 8-16 post infestation. These data provide important information on the temporal development of humoral immune responses in scabies and further supports the development of recombinant antigen based immunodiagnostic tests for recent scabies infestations.

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Related in: MedlinePlus

IgG reactivity of S. scabiei whole mite antigen extract (WMA) and Sar s 14.3 with porcine sera.A: 4–20% coomassie stained SDS-PAGE gel loaded with lane 1: prestained molecular weight marker, lane 2: WMA, 5 µg, lane 3: Sar s 14.3 (2 µg). Corresponding Western Blots probed with sera from mange infected pigs (B) and non-infected pigs (C).
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pone-0065354-g001: IgG reactivity of S. scabiei whole mite antigen extract (WMA) and Sar s 14.3 with porcine sera.A: 4–20% coomassie stained SDS-PAGE gel loaded with lane 1: prestained molecular weight marker, lane 2: WMA, 5 µg, lane 3: Sar s 14.3 (2 µg). Corresponding Western Blots probed with sera from mange infected pigs (B) and non-infected pigs (C).

Mentions: Sar s 14.3 cDNA was PCR amplified from S. scabiei var. suis using primers based on the S. scabiei var. hominis sequence. The sequenced pig mite PCR product had 100% amino acid identity to the human mite Sar s 14.3, and was submitted to Genbank (Accession KC691249). Recombinant Sar s 14.3 derived from S. scabiei var. hominis was recognised by sera from mange infected pigs, with strong IgG binding to both Sar s 14.3 and the WMA positive control (Fig. 1B). Non-infected pig sera reacted only weakly with these antigens (Fig. 1C).


Antibody responses to Sarcoptes scabiei apolipoprotein in a porcine model: relevance to immunodiagnosis of recent infection.

Rampton M, Walton SF, Holt DC, Pasay C, Kelly A, Currie BJ, McCarthy JS, Mounsey KE - PLoS ONE (2013)

IgG reactivity of S. scabiei whole mite antigen extract (WMA) and Sar s 14.3 with porcine sera.A: 4–20% coomassie stained SDS-PAGE gel loaded with lane 1: prestained molecular weight marker, lane 2: WMA, 5 µg, lane 3: Sar s 14.3 (2 µg). Corresponding Western Blots probed with sera from mange infected pigs (B) and non-infected pigs (C).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3675102&req=5

pone-0065354-g001: IgG reactivity of S. scabiei whole mite antigen extract (WMA) and Sar s 14.3 with porcine sera.A: 4–20% coomassie stained SDS-PAGE gel loaded with lane 1: prestained molecular weight marker, lane 2: WMA, 5 µg, lane 3: Sar s 14.3 (2 µg). Corresponding Western Blots probed with sera from mange infected pigs (B) and non-infected pigs (C).
Mentions: Sar s 14.3 cDNA was PCR amplified from S. scabiei var. suis using primers based on the S. scabiei var. hominis sequence. The sequenced pig mite PCR product had 100% amino acid identity to the human mite Sar s 14.3, and was submitted to Genbank (Accession KC691249). Recombinant Sar s 14.3 derived from S. scabiei var. hominis was recognised by sera from mange infected pigs, with strong IgG binding to both Sar s 14.3 and the WMA positive control (Fig. 1B). Non-infected pig sera reacted only weakly with these antigens (Fig. 1C).

Bottom Line: We utilised a porcine model to prospectively compare specific antibody responses to a primary infestation by ELISA, to Sar s 14.3 and to S. scabiei whole mite antigen extract (WMA).Differences in the antibody profile between antigens were apparent, with Sar s 14.3 responses detected earlier, and declining significantly after peak infestation compared to WMA.Both antigens resulted in >90% diagnostic sensitivity from weeks 8-16 post infestation.

View Article: PubMed Central - PubMed

Affiliation: School of Health and Sport Sciences, University of the Sunshine Coast, Maroochydore, Queensland, Australia.

ABSTRACT
No commercial immunodiagnostic tests for human scabies are currently available, and existing animal tests are not sufficiently sensitive. The recombinant Sarcoptes scabiei apolipoprotein antigen Sar s 14.3 is a promising immunodiagnostic, eliciting high levels of IgE and IgG in infected people. Limited data are available regarding the temporal development of antibodies to Sar s 14.3, an issue of relevance in terms of immunodiagnosis. We utilised a porcine model to prospectively compare specific antibody responses to a primary infestation by ELISA, to Sar s 14.3 and to S. scabiei whole mite antigen extract (WMA). Differences in the antibody profile between antigens were apparent, with Sar s 14.3 responses detected earlier, and declining significantly after peak infestation compared to WMA. Both antigens resulted in >90% diagnostic sensitivity from weeks 8-16 post infestation. These data provide important information on the temporal development of humoral immune responses in scabies and further supports the development of recombinant antigen based immunodiagnostic tests for recent scabies infestations.

Show MeSH
Related in: MedlinePlus