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Molecular epidemiology of porcine Cytomegalovirus (PCMV) in Sichuan Province, China: 2010-2012.

Liu X, Liao S, Zhu L, Xu Z, Zhou Y - PLoS ONE (2013)

Bottom Line: PCMV does not appear to have evolved into different serotypes, and two distinct sequence groups were identified (A and B).However, whether PCMV from this region has evolved into different genotypes requires further research.Analysis of the amino acid sequences confirmed the conservation of gB, but amino acid substitutions in the major epitope region have caused antigenic drift, which may have altered the immunogenicity of PCMV.

View Article: PubMed Central - PubMed

Affiliation: Animal Biotechnology Center, College of Veterinary Medicine, Sichuan Agricultural University, Ya' an, China.

ABSTRACT
Porcine cytomegalovirus (PCMV) is an immunosuppressive virus that mainly inhibits the immune function of the macrophage and T-cell lymphatic systems, and has caused huge economic losses to the porcine breeding industry. Molecular epidemiological investigation of PCMV is important for prevention and treatment, and this study is the first such investigation in Sichuan Province, Southwest China. A PCMV positive infection rate of 84.4% (865/1025) confirmed that PCMV is widely distributed in Sichuan Province. A phylogenetic tree was constructed based on the PCMV glycoprotein B gene (gB) nucleotide and amino acid sequences from 24 novel Sichuan isolates and 18 other PCMV gB sequences from Genbank. PCMV does not appear to have evolved into different serotypes, and two distinct sequence groups were identified (A and B). However, whether PCMV from this region has evolved into different genotypes requires further research. Analysis of the amino acid sequences confirmed the conservation of gB, but amino acid substitutions in the major epitope region have caused antigenic drift, which may have altered the immunogenicity of PCMV.

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Index of amino acid substitution propensity.(A) Analysis of 34 PCMV strains from China. (B) Analysis of global PCMV strains.
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pone-0064648-g005: Index of amino acid substitution propensity.(A) Analysis of 34 PCMV strains from China. (B) Analysis of global PCMV strains.

Mentions: The 18 deduced glycoprotein B amino acid sequences from the global strains were aligned and analyzed along with the novel 24 Sichuan strains. The maximum index of substitution propensity was 0.9% between the Spanish 55b strain (accession no. AF268040.2), the B6 strain (accession no. AF268039.2), the Japanese OF-1 strain (accession no. AF268041.2), the Japanese Yamaguchi strain (accession no. AB771707.1, AB771708.1, AB771706.1), and the Swedish strain (accession no. AF394056, AF394057.1), in comparison with 1.3%% between the SC strain (accession no. JN701021.1), the ZZ strain (accession no. FJ870562.1), the NB strain (accession no. FJ844360.1, FJ870561.1), the JH strain (accession no. FJ870563.1), the FJ strain (accession no. FJ870564.1), and the HN isolates (accession no.HQ686081.1, HQ686080.1, FJ595497.1, EF460488.1) (Figure 5).


Molecular epidemiology of porcine Cytomegalovirus (PCMV) in Sichuan Province, China: 2010-2012.

Liu X, Liao S, Zhu L, Xu Z, Zhou Y - PLoS ONE (2013)

Index of amino acid substitution propensity.(A) Analysis of 34 PCMV strains from China. (B) Analysis of global PCMV strains.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3675093&req=5

pone-0064648-g005: Index of amino acid substitution propensity.(A) Analysis of 34 PCMV strains from China. (B) Analysis of global PCMV strains.
Mentions: The 18 deduced glycoprotein B amino acid sequences from the global strains were aligned and analyzed along with the novel 24 Sichuan strains. The maximum index of substitution propensity was 0.9% between the Spanish 55b strain (accession no. AF268040.2), the B6 strain (accession no. AF268039.2), the Japanese OF-1 strain (accession no. AF268041.2), the Japanese Yamaguchi strain (accession no. AB771707.1, AB771708.1, AB771706.1), and the Swedish strain (accession no. AF394056, AF394057.1), in comparison with 1.3%% between the SC strain (accession no. JN701021.1), the ZZ strain (accession no. FJ870562.1), the NB strain (accession no. FJ844360.1, FJ870561.1), the JH strain (accession no. FJ870563.1), the FJ strain (accession no. FJ870564.1), and the HN isolates (accession no.HQ686081.1, HQ686080.1, FJ595497.1, EF460488.1) (Figure 5).

Bottom Line: PCMV does not appear to have evolved into different serotypes, and two distinct sequence groups were identified (A and B).However, whether PCMV from this region has evolved into different genotypes requires further research.Analysis of the amino acid sequences confirmed the conservation of gB, but amino acid substitutions in the major epitope region have caused antigenic drift, which may have altered the immunogenicity of PCMV.

View Article: PubMed Central - PubMed

Affiliation: Animal Biotechnology Center, College of Veterinary Medicine, Sichuan Agricultural University, Ya' an, China.

ABSTRACT
Porcine cytomegalovirus (PCMV) is an immunosuppressive virus that mainly inhibits the immune function of the macrophage and T-cell lymphatic systems, and has caused huge economic losses to the porcine breeding industry. Molecular epidemiological investigation of PCMV is important for prevention and treatment, and this study is the first such investigation in Sichuan Province, Southwest China. A PCMV positive infection rate of 84.4% (865/1025) confirmed that PCMV is widely distributed in Sichuan Province. A phylogenetic tree was constructed based on the PCMV glycoprotein B gene (gB) nucleotide and amino acid sequences from 24 novel Sichuan isolates and 18 other PCMV gB sequences from Genbank. PCMV does not appear to have evolved into different serotypes, and two distinct sequence groups were identified (A and B). However, whether PCMV from this region has evolved into different genotypes requires further research. Analysis of the amino acid sequences confirmed the conservation of gB, but amino acid substitutions in the major epitope region have caused antigenic drift, which may have altered the immunogenicity of PCMV.

Show MeSH
Related in: MedlinePlus