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Molecular epidemiology of porcine Cytomegalovirus (PCMV) in Sichuan Province, China: 2010-2012.

Liu X, Liao S, Zhu L, Xu Z, Zhou Y - PLoS ONE (2013)

Bottom Line: PCMV does not appear to have evolved into different serotypes, and two distinct sequence groups were identified (A and B).However, whether PCMV from this region has evolved into different genotypes requires further research.Analysis of the amino acid sequences confirmed the conservation of gB, but amino acid substitutions in the major epitope region have caused antigenic drift, which may have altered the immunogenicity of PCMV.

View Article: PubMed Central - PubMed

Affiliation: Animal Biotechnology Center, College of Veterinary Medicine, Sichuan Agricultural University, Ya' an, China.

ABSTRACT
Porcine cytomegalovirus (PCMV) is an immunosuppressive virus that mainly inhibits the immune function of the macrophage and T-cell lymphatic systems, and has caused huge economic losses to the porcine breeding industry. Molecular epidemiological investigation of PCMV is important for prevention and treatment, and this study is the first such investigation in Sichuan Province, Southwest China. A PCMV positive infection rate of 84.4% (865/1025) confirmed that PCMV is widely distributed in Sichuan Province. A phylogenetic tree was constructed based on the PCMV glycoprotein B gene (gB) nucleotide and amino acid sequences from 24 novel Sichuan isolates and 18 other PCMV gB sequences from Genbank. PCMV does not appear to have evolved into different serotypes, and two distinct sequence groups were identified (A and B). However, whether PCMV from this region has evolved into different genotypes requires further research. Analysis of the amino acid sequences confirmed the conservation of gB, but amino acid substitutions in the major epitope region have caused antigenic drift, which may have altered the immunogenicity of PCMV.

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Phylogenetic analysis of Herpesviridae viruses.Glycoprotein B nucleotide sequences of the alphaherpesviruses, betaherpesviruses, and gammaherpesviruses were obtained from GenBank: PCMV SC strain (accession no. JN701021.1); RhCMV (Rhesus cytomegalovirus; accession no. U76749.1); CyCMV (Cynomolgus macaque cytomegalovirus; accession no. HQ198248.1); MaCMV (Mycobacteriophage Barnyard; accession no. AY129339); PTCMV (Pan troglodytes cytomegalovirus; accession no. FJ538485); HCMV (Human cytomegalovirus; accession no. M6092.1); GgCMV (Gorilla cytomegalovirus; accession no. FJ538490); MuHV-2 (Murine herpesvirus type 2; accession no. AF232689.2); MCMV (Murine cytomegalovirus; M86302.1); HHV-6 (Human herpesvirus; accession no. M97927.1); PRV (Pseudorabies virus; accession no. AF257079.1; HHV-7 (Human herpesvirus; accession no. AF007830.1); AlHV-1 (Alcelaphine herpesvirus 1; accession no. AF005370); BoHV-1 (Bovine herpesvirus type 1; accession no. Z78205); BoHV-2 (Bovine herpesvirus type 2; accession no. Z78205); BoHV-4 (Bovine herpesvirus type 4; accession no. Z15044.1); EBV (Epstein-Barr virus; accession no. V01555.2); EHV-1 (Equine herpesvirus 1 strain Ab4; accession no. AY665713.1); EHV-4 (Equine herpesvirus 4 strain NS80567; accession no. AF030027.1); HHV-8 (Human herpesvirus 8 type M; accession no. U75698.1); VZV (Varicella-Zoster virus; accession no. X04370); PLHV-2 (porcine lymphotropic herpesvirus 2; accession no. AF191043); PLHV-1 (lymphotropic herpesvirus 1; accession no. AF478169.1); MuHV-2 (Murine herpesvirus 2; accession no. GU018179.1); HSV-2 (Herpes simplex virus 2; accession no. Z86099); AHV (Ateles herpesvirus; accession no. AF083424); ILTV (Laryngotracheitis Virus; accession no. X56093.1).Bootstrap values of >65% from 1000 pseudo-replicates are indicated at the branch nodes.
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pone-0064648-g002: Phylogenetic analysis of Herpesviridae viruses.Glycoprotein B nucleotide sequences of the alphaherpesviruses, betaherpesviruses, and gammaherpesviruses were obtained from GenBank: PCMV SC strain (accession no. JN701021.1); RhCMV (Rhesus cytomegalovirus; accession no. U76749.1); CyCMV (Cynomolgus macaque cytomegalovirus; accession no. HQ198248.1); MaCMV (Mycobacteriophage Barnyard; accession no. AY129339); PTCMV (Pan troglodytes cytomegalovirus; accession no. FJ538485); HCMV (Human cytomegalovirus; accession no. M6092.1); GgCMV (Gorilla cytomegalovirus; accession no. FJ538490); MuHV-2 (Murine herpesvirus type 2; accession no. AF232689.2); MCMV (Murine cytomegalovirus; M86302.1); HHV-6 (Human herpesvirus; accession no. M97927.1); PRV (Pseudorabies virus; accession no. AF257079.1; HHV-7 (Human herpesvirus; accession no. AF007830.1); AlHV-1 (Alcelaphine herpesvirus 1; accession no. AF005370); BoHV-1 (Bovine herpesvirus type 1; accession no. Z78205); BoHV-2 (Bovine herpesvirus type 2; accession no. Z78205); BoHV-4 (Bovine herpesvirus type 4; accession no. Z15044.1); EBV (Epstein-Barr virus; accession no. V01555.2); EHV-1 (Equine herpesvirus 1 strain Ab4; accession no. AY665713.1); EHV-4 (Equine herpesvirus 4 strain NS80567; accession no. AF030027.1); HHV-8 (Human herpesvirus 8 type M; accession no. U75698.1); VZV (Varicella-Zoster virus; accession no. X04370); PLHV-2 (porcine lymphotropic herpesvirus 2; accession no. AF191043); PLHV-1 (lymphotropic herpesvirus 1; accession no. AF478169.1); MuHV-2 (Murine herpesvirus 2; accession no. GU018179.1); HSV-2 (Herpes simplex virus 2; accession no. Z86099); AHV (Ateles herpesvirus; accession no. AF083424); ILTV (Laryngotracheitis Virus; accession no. X56093.1).Bootstrap values of >65% from 1000 pseudo-replicates are indicated at the branch nodes.

Mentions: The novel PCMV gB nucleotide sequences have been submitted to NCBI GenBank and assigned accession numbers KC342266–KC342289. Further Herpesviridae glycoprotein B nucleotide sequences were obtained from GenBank (http://www.ncbi.nlm.nih.gov/nuccore), Specific accession numbers are given in Figure 2 and Figure 3.


Molecular epidemiology of porcine Cytomegalovirus (PCMV) in Sichuan Province, China: 2010-2012.

Liu X, Liao S, Zhu L, Xu Z, Zhou Y - PLoS ONE (2013)

Phylogenetic analysis of Herpesviridae viruses.Glycoprotein B nucleotide sequences of the alphaherpesviruses, betaherpesviruses, and gammaherpesviruses were obtained from GenBank: PCMV SC strain (accession no. JN701021.1); RhCMV (Rhesus cytomegalovirus; accession no. U76749.1); CyCMV (Cynomolgus macaque cytomegalovirus; accession no. HQ198248.1); MaCMV (Mycobacteriophage Barnyard; accession no. AY129339); PTCMV (Pan troglodytes cytomegalovirus; accession no. FJ538485); HCMV (Human cytomegalovirus; accession no. M6092.1); GgCMV (Gorilla cytomegalovirus; accession no. FJ538490); MuHV-2 (Murine herpesvirus type 2; accession no. AF232689.2); MCMV (Murine cytomegalovirus; M86302.1); HHV-6 (Human herpesvirus; accession no. M97927.1); PRV (Pseudorabies virus; accession no. AF257079.1; HHV-7 (Human herpesvirus; accession no. AF007830.1); AlHV-1 (Alcelaphine herpesvirus 1; accession no. AF005370); BoHV-1 (Bovine herpesvirus type 1; accession no. Z78205); BoHV-2 (Bovine herpesvirus type 2; accession no. Z78205); BoHV-4 (Bovine herpesvirus type 4; accession no. Z15044.1); EBV (Epstein-Barr virus; accession no. V01555.2); EHV-1 (Equine herpesvirus 1 strain Ab4; accession no. AY665713.1); EHV-4 (Equine herpesvirus 4 strain NS80567; accession no. AF030027.1); HHV-8 (Human herpesvirus 8 type M; accession no. U75698.1); VZV (Varicella-Zoster virus; accession no. X04370); PLHV-2 (porcine lymphotropic herpesvirus 2; accession no. AF191043); PLHV-1 (lymphotropic herpesvirus 1; accession no. AF478169.1); MuHV-2 (Murine herpesvirus 2; accession no. GU018179.1); HSV-2 (Herpes simplex virus 2; accession no. Z86099); AHV (Ateles herpesvirus; accession no. AF083424); ILTV (Laryngotracheitis Virus; accession no. X56093.1).Bootstrap values of >65% from 1000 pseudo-replicates are indicated at the branch nodes.
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Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3675093&req=5

pone-0064648-g002: Phylogenetic analysis of Herpesviridae viruses.Glycoprotein B nucleotide sequences of the alphaherpesviruses, betaherpesviruses, and gammaherpesviruses were obtained from GenBank: PCMV SC strain (accession no. JN701021.1); RhCMV (Rhesus cytomegalovirus; accession no. U76749.1); CyCMV (Cynomolgus macaque cytomegalovirus; accession no. HQ198248.1); MaCMV (Mycobacteriophage Barnyard; accession no. AY129339); PTCMV (Pan troglodytes cytomegalovirus; accession no. FJ538485); HCMV (Human cytomegalovirus; accession no. M6092.1); GgCMV (Gorilla cytomegalovirus; accession no. FJ538490); MuHV-2 (Murine herpesvirus type 2; accession no. AF232689.2); MCMV (Murine cytomegalovirus; M86302.1); HHV-6 (Human herpesvirus; accession no. M97927.1); PRV (Pseudorabies virus; accession no. AF257079.1; HHV-7 (Human herpesvirus; accession no. AF007830.1); AlHV-1 (Alcelaphine herpesvirus 1; accession no. AF005370); BoHV-1 (Bovine herpesvirus type 1; accession no. Z78205); BoHV-2 (Bovine herpesvirus type 2; accession no. Z78205); BoHV-4 (Bovine herpesvirus type 4; accession no. Z15044.1); EBV (Epstein-Barr virus; accession no. V01555.2); EHV-1 (Equine herpesvirus 1 strain Ab4; accession no. AY665713.1); EHV-4 (Equine herpesvirus 4 strain NS80567; accession no. AF030027.1); HHV-8 (Human herpesvirus 8 type M; accession no. U75698.1); VZV (Varicella-Zoster virus; accession no. X04370); PLHV-2 (porcine lymphotropic herpesvirus 2; accession no. AF191043); PLHV-1 (lymphotropic herpesvirus 1; accession no. AF478169.1); MuHV-2 (Murine herpesvirus 2; accession no. GU018179.1); HSV-2 (Herpes simplex virus 2; accession no. Z86099); AHV (Ateles herpesvirus; accession no. AF083424); ILTV (Laryngotracheitis Virus; accession no. X56093.1).Bootstrap values of >65% from 1000 pseudo-replicates are indicated at the branch nodes.
Mentions: The novel PCMV gB nucleotide sequences have been submitted to NCBI GenBank and assigned accession numbers KC342266–KC342289. Further Herpesviridae glycoprotein B nucleotide sequences were obtained from GenBank (http://www.ncbi.nlm.nih.gov/nuccore), Specific accession numbers are given in Figure 2 and Figure 3.

Bottom Line: PCMV does not appear to have evolved into different serotypes, and two distinct sequence groups were identified (A and B).However, whether PCMV from this region has evolved into different genotypes requires further research.Analysis of the amino acid sequences confirmed the conservation of gB, but amino acid substitutions in the major epitope region have caused antigenic drift, which may have altered the immunogenicity of PCMV.

View Article: PubMed Central - PubMed

Affiliation: Animal Biotechnology Center, College of Veterinary Medicine, Sichuan Agricultural University, Ya' an, China.

ABSTRACT
Porcine cytomegalovirus (PCMV) is an immunosuppressive virus that mainly inhibits the immune function of the macrophage and T-cell lymphatic systems, and has caused huge economic losses to the porcine breeding industry. Molecular epidemiological investigation of PCMV is important for prevention and treatment, and this study is the first such investigation in Sichuan Province, Southwest China. A PCMV positive infection rate of 84.4% (865/1025) confirmed that PCMV is widely distributed in Sichuan Province. A phylogenetic tree was constructed based on the PCMV glycoprotein B gene (gB) nucleotide and amino acid sequences from 24 novel Sichuan isolates and 18 other PCMV gB sequences from Genbank. PCMV does not appear to have evolved into different serotypes, and two distinct sequence groups were identified (A and B). However, whether PCMV from this region has evolved into different genotypes requires further research. Analysis of the amino acid sequences confirmed the conservation of gB, but amino acid substitutions in the major epitope region have caused antigenic drift, which may have altered the immunogenicity of PCMV.

Show MeSH
Related in: MedlinePlus