Limits...
A mechanochemical model of cell reorientation on substrates under cyclic stretch.

Qian J, Liu H, Lin Y, Chen W, Gao H - PLoS ONE (2013)

Bottom Line: We report a theoretical study on the cyclic stretch-induced reorientation of spindle-shaped cells.Our main hypothesis is that cells tend to orient in the direction where the formation of stress fibers is energetically most favorable.This theory also provides a simple explanation on the regulation of protein Rho in the formation of stretch-induced stress fibers in cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Engineering Mechanics, Soft Matter Research Center, Zhejiang University, Hangzhou, Zhejiang, China.

ABSTRACT
We report a theoretical study on the cyclic stretch-induced reorientation of spindle-shaped cells. Specifically, by taking into account the evolution of sub-cellular structures like the contractile stress fibers and adhesive receptor-ligand clusters, we develop a mechanochemical model to describe the dynamics of cell realignment in response to cyclically stretched substrates. Our main hypothesis is that cells tend to orient in the direction where the formation of stress fibers is energetically most favorable. We show that, when subjected to cyclic stretch, the final alignment of cells reflects the competition between the elevated force within stress fibers that accelerates their disassembly and the disruption of cell-substrate adhesion as well, and an effectively increased substrate rigidity that promotes more stable focal adhesions. Our model predictions are consistent with various observations like the substrate rigidity dependent formation of stable adhesions and the stretching frequency, as well as stretching amplitude, dependence of cell realignment. This theory also provides a simple explanation on the regulation of protein Rho in the formation of stretch-induced stress fibers in cells.

Show MeSH

Related in: MedlinePlus

Long-time average filament density  as a function of the cell orientation angle  under different values of stretching amplitude (stretch frequency: 1 Hz).
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3675090&req=5

pone-0065864-g008: Long-time average filament density as a function of the cell orientation angle under different values of stretching amplitude (stretch frequency: 1 Hz).

Mentions: Alternatively, when the stretching frequency is fixed at 1 Hz, Fig. 8 shows the variation of as functions of cell orientation under different stretching amplitudes. An immediate observation is that becomes insensitive to when the stretching amplitude is small (∼1%), suggesting that cells do not have a preferable orientation in this case. Interestingly, it has been reported that cells indeed do not respond to stretches with amplitude less than ∼1–2% [4], [7]–[14].


A mechanochemical model of cell reorientation on substrates under cyclic stretch.

Qian J, Liu H, Lin Y, Chen W, Gao H - PLoS ONE (2013)

Long-time average filament density  as a function of the cell orientation angle  under different values of stretching amplitude (stretch frequency: 1 Hz).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3675090&req=5

pone-0065864-g008: Long-time average filament density as a function of the cell orientation angle under different values of stretching amplitude (stretch frequency: 1 Hz).
Mentions: Alternatively, when the stretching frequency is fixed at 1 Hz, Fig. 8 shows the variation of as functions of cell orientation under different stretching amplitudes. An immediate observation is that becomes insensitive to when the stretching amplitude is small (∼1%), suggesting that cells do not have a preferable orientation in this case. Interestingly, it has been reported that cells indeed do not respond to stretches with amplitude less than ∼1–2% [4], [7]–[14].

Bottom Line: We report a theoretical study on the cyclic stretch-induced reorientation of spindle-shaped cells.Our main hypothesis is that cells tend to orient in the direction where the formation of stress fibers is energetically most favorable.This theory also provides a simple explanation on the regulation of protein Rho in the formation of stretch-induced stress fibers in cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Engineering Mechanics, Soft Matter Research Center, Zhejiang University, Hangzhou, Zhejiang, China.

ABSTRACT
We report a theoretical study on the cyclic stretch-induced reorientation of spindle-shaped cells. Specifically, by taking into account the evolution of sub-cellular structures like the contractile stress fibers and adhesive receptor-ligand clusters, we develop a mechanochemical model to describe the dynamics of cell realignment in response to cyclically stretched substrates. Our main hypothesis is that cells tend to orient in the direction where the formation of stress fibers is energetically most favorable. We show that, when subjected to cyclic stretch, the final alignment of cells reflects the competition between the elevated force within stress fibers that accelerates their disassembly and the disruption of cell-substrate adhesion as well, and an effectively increased substrate rigidity that promotes more stable focal adhesions. Our model predictions are consistent with various observations like the substrate rigidity dependent formation of stable adhesions and the stretching frequency, as well as stretching amplitude, dependence of cell realignment. This theory also provides a simple explanation on the regulation of protein Rho in the formation of stretch-induced stress fibers in cells.

Show MeSH
Related in: MedlinePlus