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Spray nozzles, pressures, additives and stirring time on viability and pathogenicity of entomopathogenic nematodes (nematoda: rhabditida) for greenhouses.

Moreira GF, Batista ES, Campos HB, Lemos RE, Ferreira Mda C - PLoS ONE (2013)

Bottom Line: The different nozzles, at pressures of up to 620 kPa, were found to be compatible with S. feltiae.Vegetable oil, mineral oil and molasses were found to be compatible adjuvants for S. feltiae, and stirring in a motorized backpack sprayer for 30 minutes did not impact the viability or pathogenicity of this nematode.Appropriate techniques for the application of nematodes with backpack sprayers are discussed.

View Article: PubMed Central - PubMed

Affiliation: Department of Fitossanidade, Universidade Estadual Paulista (UNESP), Jaboticabal Campus, Jaboticabal, São Paulo, Brazil. grabiologia@yahoo.com.br

ABSTRACT
The objective of this study was to evaluate different strategies for the application of entomopathogenic nematodes (EPN). Three different models of spray nozzles with air induction (AI 11003, TTI 11003 and AD-IA 11004), three spray pressures (207, 413 and 720 kPa), four different additives for tank mixtures (cane molasses, mineral oil, vegetable oil and glycerin) and the influence of tank mixture stirring time were all evaluated for their effect on EPN (Steinernema feltiae) viability and pathogenicity. The different nozzles, at pressures of up to 620 kPa, were found to be compatible with S. feltiae. Vegetable oil, mineral oil and molasses were found to be compatible adjuvants for S. feltiae, and stirring in a motorized backpack sprayer for 30 minutes did not impact the viability or pathogenicity of this nematode. Appropriate techniques for the application of nematodes with backpack sprayers are discussed.

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Compatibility of spray with different adjuvants on viability and pathogenicity of Steinernema feltiae.
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pone-0065759-g001: Compatibility of spray with different adjuvants on viability and pathogenicity of Steinernema feltiae.

Mentions: From the various adjuvants used, vegetable oil, mineral oil and molasses were shown to be compatible with S. feltiae, with the IJ viability of 97%, not differing significantly from the control treatment (95%). The lowest viability (77%) was obtained in the tank mixture containing glycerin-based treatments, which differed significantly from the other ones. Concerning pathogenicity, however, there was no significant difference between treatments, and the glycerin-based treatments had a 93% mortality rate with the G. mellonella larvae (Figure 1).


Spray nozzles, pressures, additives and stirring time on viability and pathogenicity of entomopathogenic nematodes (nematoda: rhabditida) for greenhouses.

Moreira GF, Batista ES, Campos HB, Lemos RE, Ferreira Mda C - PLoS ONE (2013)

Compatibility of spray with different adjuvants on viability and pathogenicity of Steinernema feltiae.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3675033&req=5

pone-0065759-g001: Compatibility of spray with different adjuvants on viability and pathogenicity of Steinernema feltiae.
Mentions: From the various adjuvants used, vegetable oil, mineral oil and molasses were shown to be compatible with S. feltiae, with the IJ viability of 97%, not differing significantly from the control treatment (95%). The lowest viability (77%) was obtained in the tank mixture containing glycerin-based treatments, which differed significantly from the other ones. Concerning pathogenicity, however, there was no significant difference between treatments, and the glycerin-based treatments had a 93% mortality rate with the G. mellonella larvae (Figure 1).

Bottom Line: The different nozzles, at pressures of up to 620 kPa, were found to be compatible with S. feltiae.Vegetable oil, mineral oil and molasses were found to be compatible adjuvants for S. feltiae, and stirring in a motorized backpack sprayer for 30 minutes did not impact the viability or pathogenicity of this nematode.Appropriate techniques for the application of nematodes with backpack sprayers are discussed.

View Article: PubMed Central - PubMed

Affiliation: Department of Fitossanidade, Universidade Estadual Paulista (UNESP), Jaboticabal Campus, Jaboticabal, São Paulo, Brazil. grabiologia@yahoo.com.br

ABSTRACT
The objective of this study was to evaluate different strategies for the application of entomopathogenic nematodes (EPN). Three different models of spray nozzles with air induction (AI 11003, TTI 11003 and AD-IA 11004), three spray pressures (207, 413 and 720 kPa), four different additives for tank mixtures (cane molasses, mineral oil, vegetable oil and glycerin) and the influence of tank mixture stirring time were all evaluated for their effect on EPN (Steinernema feltiae) viability and pathogenicity. The different nozzles, at pressures of up to 620 kPa, were found to be compatible with S. feltiae. Vegetable oil, mineral oil and molasses were found to be compatible adjuvants for S. feltiae, and stirring in a motorized backpack sprayer for 30 minutes did not impact the viability or pathogenicity of this nematode. Appropriate techniques for the application of nematodes with backpack sprayers are discussed.

Show MeSH
Related in: MedlinePlus