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Frequency of regulatory T-cells in the peripheral blood of patients with pulmonary tuberculosis from shanxi province, china.

Pang H, Yu Q, Guo B, Jiang Y, Wan L, Li J, Wu Y, Wan K - PLoS ONE (2013)

Bottom Line: Frequencyof natural regulatory T cells and inductive regulatory T cells in the peripheral blood samples from Group 1 patients were all significantly higher (P<0.05) than those from Groups 2 and 3.Our results indicate that frequency of natural regulatory T cells and inductive regulatory T cells are significantly higher in the peripheral blood of patients with active pulmonary tuberculosis.These findings have potential application in improving TB diagnostic methods.

View Article: PubMed Central - PubMed

Affiliation: Department of Immunology, Changzhi Medical College, Changzhi, Shanxi Province, PR China.

ABSTRACT

Background: Tuberculosis (TB) is a disease caused by the chronic and continuous infection of the pathogen Mycobacterium tuberculosis (M. tuberculosis). M. tuberculosis is an intracellular bacterial pathogen and is eliminated mainly through CD4(+) effector Th cells. M. tuberculosis induces regulatory T lymphocytes (Tregs) that mediate immune suppression by cell-to-cell contact or by secreting cytokines such as transforming growth factor-β (TGF-β). To understand the role of regulatory T-cells in the pathogenesis of TB, we have measured the in vivo frequency of regulatory T-cells and associated in vivo cytokine production in pulmonary tuberculosis patients.

Methodology/principal findings: In this study, we analyzed blood samples from 3 different populations (Group 1: patients with active TB, Group 2: patients recovered from TB and Group 3: healthy controls). We measured natural regulatory T-cell expression in peripheral blood using flow cytometry, and levels of blood serum IFN-γ and TGF-β1 using ELISA. The in vivo function of inductive regulatory T cells was mainly indicated by the expression of IFN-γ, TGF-β1, etc. Frequencyof natural regulatory T cells and inductive regulatory T cells in the peripheral blood samples from Group 1 patients were all significantly higher (P<0.05) than those from Groups 2 and 3.

Conclusion/significance: Our results indicate that frequency of natural regulatory T cells and inductive regulatory T cells are significantly higher in the peripheral blood of patients with active pulmonary tuberculosis. These findings have potential application in improving TB diagnostic methods.

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Related in: MedlinePlus

Representative flow plots from a recovered TB patient labeled with anti-CD4, CD25 and Foxp3.Appropriate isotype controls were included to establish the positive gates. (I) FSC and SSC to identify lymphocytes. (II) CD4+ cells within the lymphocyte gate. (III) Cells were gated on the CD4+ cells shown in B and CD4+CD25+ cells were determined. (IV) Cells were gated on CD4+CD25+ cells and Foxp3 expression was determined.
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pone-0065496-g002: Representative flow plots from a recovered TB patient labeled with anti-CD4, CD25 and Foxp3.Appropriate isotype controls were included to establish the positive gates. (I) FSC and SSC to identify lymphocytes. (II) CD4+ cells within the lymphocyte gate. (III) Cells were gated on the CD4+ cells shown in B and CD4+CD25+ cells were determined. (IV) Cells were gated on CD4+CD25+ cells and Foxp3 expression was determined.

Mentions: Flow cytometry was used to detect CD4+CD25+ lymphocytes and CD4+CD25+Foxp3+ lymphocytes. Results for Group 1, Group 2 and Group 3 are shown in Figures 1, 2, 3, respectively; in the figures, L, A, n and P2 representing lymphocytes, CD4+ cells, CD4+CD25+ cells and CD4+CD25+Foxp3+ cells, respectively.


Frequency of regulatory T-cells in the peripheral blood of patients with pulmonary tuberculosis from shanxi province, china.

Pang H, Yu Q, Guo B, Jiang Y, Wan L, Li J, Wu Y, Wan K - PLoS ONE (2013)

Representative flow plots from a recovered TB patient labeled with anti-CD4, CD25 and Foxp3.Appropriate isotype controls were included to establish the positive gates. (I) FSC and SSC to identify lymphocytes. (II) CD4+ cells within the lymphocyte gate. (III) Cells were gated on the CD4+ cells shown in B and CD4+CD25+ cells were determined. (IV) Cells were gated on CD4+CD25+ cells and Foxp3 expression was determined.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3675031&req=5

pone-0065496-g002: Representative flow plots from a recovered TB patient labeled with anti-CD4, CD25 and Foxp3.Appropriate isotype controls were included to establish the positive gates. (I) FSC and SSC to identify lymphocytes. (II) CD4+ cells within the lymphocyte gate. (III) Cells were gated on the CD4+ cells shown in B and CD4+CD25+ cells were determined. (IV) Cells were gated on CD4+CD25+ cells and Foxp3 expression was determined.
Mentions: Flow cytometry was used to detect CD4+CD25+ lymphocytes and CD4+CD25+Foxp3+ lymphocytes. Results for Group 1, Group 2 and Group 3 are shown in Figures 1, 2, 3, respectively; in the figures, L, A, n and P2 representing lymphocytes, CD4+ cells, CD4+CD25+ cells and CD4+CD25+Foxp3+ cells, respectively.

Bottom Line: Frequencyof natural regulatory T cells and inductive regulatory T cells in the peripheral blood samples from Group 1 patients were all significantly higher (P<0.05) than those from Groups 2 and 3.Our results indicate that frequency of natural regulatory T cells and inductive regulatory T cells are significantly higher in the peripheral blood of patients with active pulmonary tuberculosis.These findings have potential application in improving TB diagnostic methods.

View Article: PubMed Central - PubMed

Affiliation: Department of Immunology, Changzhi Medical College, Changzhi, Shanxi Province, PR China.

ABSTRACT

Background: Tuberculosis (TB) is a disease caused by the chronic and continuous infection of the pathogen Mycobacterium tuberculosis (M. tuberculosis). M. tuberculosis is an intracellular bacterial pathogen and is eliminated mainly through CD4(+) effector Th cells. M. tuberculosis induces regulatory T lymphocytes (Tregs) that mediate immune suppression by cell-to-cell contact or by secreting cytokines such as transforming growth factor-β (TGF-β). To understand the role of regulatory T-cells in the pathogenesis of TB, we have measured the in vivo frequency of regulatory T-cells and associated in vivo cytokine production in pulmonary tuberculosis patients.

Methodology/principal findings: In this study, we analyzed blood samples from 3 different populations (Group 1: patients with active TB, Group 2: patients recovered from TB and Group 3: healthy controls). We measured natural regulatory T-cell expression in peripheral blood using flow cytometry, and levels of blood serum IFN-γ and TGF-β1 using ELISA. The in vivo function of inductive regulatory T cells was mainly indicated by the expression of IFN-γ, TGF-β1, etc. Frequencyof natural regulatory T cells and inductive regulatory T cells in the peripheral blood samples from Group 1 patients were all significantly higher (P<0.05) than those from Groups 2 and 3.

Conclusion/significance: Our results indicate that frequency of natural regulatory T cells and inductive regulatory T cells are significantly higher in the peripheral blood of patients with active pulmonary tuberculosis. These findings have potential application in improving TB diagnostic methods.

Show MeSH
Related in: MedlinePlus