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Metabonomic profiling of serum and urine by (1)H NMR-based spectroscopy discriminates patients with chronic obstructive pulmonary disease and healthy individuals.

Wang L, Tang Y, Liu S, Mao S, Ling Y, Liu D, He X, Wang X - PLoS ONE (2013)

Bottom Line: Samples were analyzed by high resolution (1)H NMR (600 MHz), and the obtained spectral profiles were then subjected to multivariate data analysis.Moreover, metabolic differences in urine were more significant than in serum.Conversely, acetate, ketone bodies, carnosine, m-hydroxyphenylacetate, phenylacetyglycine, pyruvate and α-ketoglutarate exhibited enhanced expression levels in COPD patients relative to healthy subjects.

View Article: PubMed Central - PubMed

Affiliation: Department of Respiratory Medicine, The Fourth Affiliated Hospital of China Medical University, Shenyang, People's Republic of China.

ABSTRACT
Chronic obstructive pulmonary disease (COPD) has seriously impacted the health of individuals and populations. In this study, proton nuclear magnetic resonance ((1)H NMR)-based metabonomics combined with multivariate pattern recognition analysis was applied to investigate the metabolic signatures of patients with COPD. Serum and urine samples were collected from COPD patients (n = 32) and healthy controls (n = 21), respectively. Samples were analyzed by high resolution (1)H NMR (600 MHz), and the obtained spectral profiles were then subjected to multivariate data analysis. Consistent metabolic differences have been found in serum as well as in urine samples from COPD patients and healthy controls. Compared to healthy controls, COPD patients displayed decreased lipoprotein and amino acids, including branched-chain amino acids (BCAAs), and increased glycerolphosphocholine in serum. Moreover, metabolic differences in urine were more significant than in serum. Decreased urinary 1-methylnicotinamide, creatinine and lactate have been discovered in COPD patients in comparison with healthy controls. Conversely, acetate, ketone bodies, carnosine, m-hydroxyphenylacetate, phenylacetyglycine, pyruvate and α-ketoglutarate exhibited enhanced expression levels in COPD patients relative to healthy subjects. Our results illustrate the potential application of NMR-based metabonomics in early diagnosis and understanding the mechanisms of COPD.

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Representative NOESYPR1D 1H NMR spectra of urine obtained from (A) controls and (B) COPD patients.The region of δ 5.0–9.5 (in the dashed box) was magnified 16 times compared with corresponding region of δ 0.5–4.5 for the purpose of clarity. Keys: 1-MN: 1-Methylnicotinamide; Ace: Acetate; Act: Acetone; Ala: Alanine; All: Allantoin; AD: Acetamide; Bu: Butyrate; Car: Carnosine; Cit: Citrate; Cho: Choline; Cn: Creatinine; DMA: Dimethylamine; DMG: Dimethylglycine; For: Formate; Gly: Glycine; Hip: Hippurate; IB: Isobutyrate; Kg: α-Ketoglutarate; Lac: Lactate; MM: Methylmalonate; N-MN: N-Methylnicotinamide; NAG: N-Acetylglutamate; PAG: Phenylacetyglycine; p-HPA: p-Hydroxyphenylacetate; Py: Pyruvate; Pyr: Pyridoxine; Suc: Succinate; Tau: Taurine; TMA: Trimethylamine; TMAO: Trimethylamine-N-Oxide; U: Unknown; α-HB: α-Hydroxybutyrate; α-HIB: α-Hydrxoy-isobutyrate.
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pone-0065675-g002: Representative NOESYPR1D 1H NMR spectra of urine obtained from (A) controls and (B) COPD patients.The region of δ 5.0–9.5 (in the dashed box) was magnified 16 times compared with corresponding region of δ 0.5–4.5 for the purpose of clarity. Keys: 1-MN: 1-Methylnicotinamide; Ace: Acetate; Act: Acetone; Ala: Alanine; All: Allantoin; AD: Acetamide; Bu: Butyrate; Car: Carnosine; Cit: Citrate; Cho: Choline; Cn: Creatinine; DMA: Dimethylamine; DMG: Dimethylglycine; For: Formate; Gly: Glycine; Hip: Hippurate; IB: Isobutyrate; Kg: α-Ketoglutarate; Lac: Lactate; MM: Methylmalonate; N-MN: N-Methylnicotinamide; NAG: N-Acetylglutamate; PAG: Phenylacetyglycine; p-HPA: p-Hydroxyphenylacetate; Py: Pyruvate; Pyr: Pyridoxine; Suc: Succinate; Tau: Taurine; TMA: Trimethylamine; TMAO: Trimethylamine-N-Oxide; U: Unknown; α-HB: α-Hydroxybutyrate; α-HIB: α-Hydrxoy-isobutyrate.

Mentions: Serum and urine samples were collected from COPD patients (n = 32) and healthy controls (n = 21), respectively. Representative 600 MHz 1H NMR spectra of serum and urine samples from a COPD patient and a healthy subject are shown in Figure 1 and 2, respectively. Abundant endogenous metabolites were detected both in serum and urine according to these spectral data. The dominant metabolites detected in serum were lipoproteins and several amino acids (e.g., alanine, lysine, glutamine, glycine, histidine) (Figure 1). The most intense signals in urine arose from glycine, creatinine, citrate, α-ketoglutarate, trimethylamine-N-oxide and p-hydroxyphenylacetate (Figure 2). Due to the high interindividual variability and large complexity, it’s impossible to get consistent comparison results across all the subjects. Thus, the NMR spectra of serum and urine samples were subjected to multivariate statistical data analysis in order to identify the metabolic differences between COPD patients and healthy controls.


Metabonomic profiling of serum and urine by (1)H NMR-based spectroscopy discriminates patients with chronic obstructive pulmonary disease and healthy individuals.

Wang L, Tang Y, Liu S, Mao S, Ling Y, Liu D, He X, Wang X - PLoS ONE (2013)

Representative NOESYPR1D 1H NMR spectra of urine obtained from (A) controls and (B) COPD patients.The region of δ 5.0–9.5 (in the dashed box) was magnified 16 times compared with corresponding region of δ 0.5–4.5 for the purpose of clarity. Keys: 1-MN: 1-Methylnicotinamide; Ace: Acetate; Act: Acetone; Ala: Alanine; All: Allantoin; AD: Acetamide; Bu: Butyrate; Car: Carnosine; Cit: Citrate; Cho: Choline; Cn: Creatinine; DMA: Dimethylamine; DMG: Dimethylglycine; For: Formate; Gly: Glycine; Hip: Hippurate; IB: Isobutyrate; Kg: α-Ketoglutarate; Lac: Lactate; MM: Methylmalonate; N-MN: N-Methylnicotinamide; NAG: N-Acetylglutamate; PAG: Phenylacetyglycine; p-HPA: p-Hydroxyphenylacetate; Py: Pyruvate; Pyr: Pyridoxine; Suc: Succinate; Tau: Taurine; TMA: Trimethylamine; TMAO: Trimethylamine-N-Oxide; U: Unknown; α-HB: α-Hydroxybutyrate; α-HIB: α-Hydrxoy-isobutyrate.
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pone-0065675-g002: Representative NOESYPR1D 1H NMR spectra of urine obtained from (A) controls and (B) COPD patients.The region of δ 5.0–9.5 (in the dashed box) was magnified 16 times compared with corresponding region of δ 0.5–4.5 for the purpose of clarity. Keys: 1-MN: 1-Methylnicotinamide; Ace: Acetate; Act: Acetone; Ala: Alanine; All: Allantoin; AD: Acetamide; Bu: Butyrate; Car: Carnosine; Cit: Citrate; Cho: Choline; Cn: Creatinine; DMA: Dimethylamine; DMG: Dimethylglycine; For: Formate; Gly: Glycine; Hip: Hippurate; IB: Isobutyrate; Kg: α-Ketoglutarate; Lac: Lactate; MM: Methylmalonate; N-MN: N-Methylnicotinamide; NAG: N-Acetylglutamate; PAG: Phenylacetyglycine; p-HPA: p-Hydroxyphenylacetate; Py: Pyruvate; Pyr: Pyridoxine; Suc: Succinate; Tau: Taurine; TMA: Trimethylamine; TMAO: Trimethylamine-N-Oxide; U: Unknown; α-HB: α-Hydroxybutyrate; α-HIB: α-Hydrxoy-isobutyrate.
Mentions: Serum and urine samples were collected from COPD patients (n = 32) and healthy controls (n = 21), respectively. Representative 600 MHz 1H NMR spectra of serum and urine samples from a COPD patient and a healthy subject are shown in Figure 1 and 2, respectively. Abundant endogenous metabolites were detected both in serum and urine according to these spectral data. The dominant metabolites detected in serum were lipoproteins and several amino acids (e.g., alanine, lysine, glutamine, glycine, histidine) (Figure 1). The most intense signals in urine arose from glycine, creatinine, citrate, α-ketoglutarate, trimethylamine-N-oxide and p-hydroxyphenylacetate (Figure 2). Due to the high interindividual variability and large complexity, it’s impossible to get consistent comparison results across all the subjects. Thus, the NMR spectra of serum and urine samples were subjected to multivariate statistical data analysis in order to identify the metabolic differences between COPD patients and healthy controls.

Bottom Line: Samples were analyzed by high resolution (1)H NMR (600 MHz), and the obtained spectral profiles were then subjected to multivariate data analysis.Moreover, metabolic differences in urine were more significant than in serum.Conversely, acetate, ketone bodies, carnosine, m-hydroxyphenylacetate, phenylacetyglycine, pyruvate and α-ketoglutarate exhibited enhanced expression levels in COPD patients relative to healthy subjects.

View Article: PubMed Central - PubMed

Affiliation: Department of Respiratory Medicine, The Fourth Affiliated Hospital of China Medical University, Shenyang, People's Republic of China.

ABSTRACT
Chronic obstructive pulmonary disease (COPD) has seriously impacted the health of individuals and populations. In this study, proton nuclear magnetic resonance ((1)H NMR)-based metabonomics combined with multivariate pattern recognition analysis was applied to investigate the metabolic signatures of patients with COPD. Serum and urine samples were collected from COPD patients (n = 32) and healthy controls (n = 21), respectively. Samples were analyzed by high resolution (1)H NMR (600 MHz), and the obtained spectral profiles were then subjected to multivariate data analysis. Consistent metabolic differences have been found in serum as well as in urine samples from COPD patients and healthy controls. Compared to healthy controls, COPD patients displayed decreased lipoprotein and amino acids, including branched-chain amino acids (BCAAs), and increased glycerolphosphocholine in serum. Moreover, metabolic differences in urine were more significant than in serum. Decreased urinary 1-methylnicotinamide, creatinine and lactate have been discovered in COPD patients in comparison with healthy controls. Conversely, acetate, ketone bodies, carnosine, m-hydroxyphenylacetate, phenylacetyglycine, pyruvate and α-ketoglutarate exhibited enhanced expression levels in COPD patients relative to healthy subjects. Our results illustrate the potential application of NMR-based metabonomics in early diagnosis and understanding the mechanisms of COPD.

Show MeSH
Related in: MedlinePlus