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Replacing a native Wolbachia with a novel strain results in an increase in endosymbiont load and resistance to dengue virus in a mosquito vector.

Bian G, Zhou G, Lu P, Xi Z - PLoS Negl Trop Dis (2013)

Bottom Line: In a previous study, the native wPolA in Ae. polynesiensis was replaced with wAlbB from Ae. albopictus, and resulted in the generation of the transinfected "MTB" strain with low susceptibility for filarial worms.The results show that wAlbB can induce a strong resistance to DENV-2 in the MTB mosquito.Evidence also supports that this resistance is related to a dramatic increase in Wolbachia density in the MTB's somatic tissues, including the midgut and salivary gland.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan, United States of America.

ABSTRACT
Wolbachia is a maternally transmitted endosymbiotic bacterium that is estimated to infect up to 65% of insect species. The ability of Wolbachia to both induce pathogen interference and spread into mosquito vector populations makes it possible to develop Wolbachia as a biological control agent for vector-borne disease control. Although Wolbachia induces resistance to dengue virus (DENV), filarial worms, and Plasmodium in mosquitoes, species like Aedes polynesiensis and Aedes albopictus, which carry native Wolbachia infections, are able to transmit dengue and filariasis. In a previous study, the native wPolA in Ae. polynesiensis was replaced with wAlbB from Ae. albopictus, and resulted in the generation of the transinfected "MTB" strain with low susceptibility for filarial worms. In this study, we compare the dynamics of DENV serotype 2 (DENV-2) within the wild type "APM" strain and the MTB strain of Ae. polynesiensis by measuring viral infection in the mosquito whole body, midgut, head, and saliva at different time points post infection. The results show that wAlbB can induce a strong resistance to DENV-2 in the MTB mosquito. Evidence also supports that this resistance is related to a dramatic increase in Wolbachia density in the MTB's somatic tissues, including the midgut and salivary gland. Our results suggests that replacement of a native Wolbachia with a novel infection could serve as a strategy for developing a Wolbachia-based approach to target naturally infected insects for vector-borne disease control.

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Resistance of MTB mosquitoes to DENV-2 is associated with a high density of Wolbachia in mosquito somatic tissues.The fold change in genome copy of the Wolbachia surface protein (WSP) gene in MTB mosquitoes is compared to APM mosquitoes. The copy number of the Wolbachia wsp was normalized by Ae. polynesiensis RPS6. In all the assays, the midguts, salivary glands, fat bodies, and ovaries of 7-day-old non-blood-fed females were dissected and used for extraction of total genomic DNA. Error bars are standard errors of the mean of twelve biological replicates.
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pntd-0002250-g004: Resistance of MTB mosquitoes to DENV-2 is associated with a high density of Wolbachia in mosquito somatic tissues.The fold change in genome copy of the Wolbachia surface protein (WSP) gene in MTB mosquitoes is compared to APM mosquitoes. The copy number of the Wolbachia wsp was normalized by Ae. polynesiensis RPS6. In all the assays, the midguts, salivary glands, fat bodies, and ovaries of 7-day-old non-blood-fed females were dissected and used for extraction of total genomic DNA. Error bars are standard errors of the mean of twelve biological replicates.

Mentions: We previously found that Wolbachia can induce resistance to dengue infection in a Wolbachia-density dependent and a tissue-specific manner [18]. To test whether the above viral inhibition is caused by an increased Wolbachia density in the somatic tissues of MTB mosquitoes, we compared the Wolbachia density between MTB and APM mosquitoes using qPCR. We found that the Wolbachia density is significantly higher in the salivary glands (5,738-fold), fat bodies (68-fold), and midguts (269-fold) of MTB mosquitoes as compared to APM mosquitoes, while no differences were observed in the ovaries (1-fold) of APM and MTB mosquitoes (Fig. 4). These results suggest that the high density of Wolbachia in somatic tissues of MTB mosquitoes may contribute directly to their low susceptibility for DENV.


Replacing a native Wolbachia with a novel strain results in an increase in endosymbiont load and resistance to dengue virus in a mosquito vector.

Bian G, Zhou G, Lu P, Xi Z - PLoS Negl Trop Dis (2013)

Resistance of MTB mosquitoes to DENV-2 is associated with a high density of Wolbachia in mosquito somatic tissues.The fold change in genome copy of the Wolbachia surface protein (WSP) gene in MTB mosquitoes is compared to APM mosquitoes. The copy number of the Wolbachia wsp was normalized by Ae. polynesiensis RPS6. In all the assays, the midguts, salivary glands, fat bodies, and ovaries of 7-day-old non-blood-fed females were dissected and used for extraction of total genomic DNA. Error bars are standard errors of the mean of twelve biological replicates.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3675004&req=5

pntd-0002250-g004: Resistance of MTB mosquitoes to DENV-2 is associated with a high density of Wolbachia in mosquito somatic tissues.The fold change in genome copy of the Wolbachia surface protein (WSP) gene in MTB mosquitoes is compared to APM mosquitoes. The copy number of the Wolbachia wsp was normalized by Ae. polynesiensis RPS6. In all the assays, the midguts, salivary glands, fat bodies, and ovaries of 7-day-old non-blood-fed females were dissected and used for extraction of total genomic DNA. Error bars are standard errors of the mean of twelve biological replicates.
Mentions: We previously found that Wolbachia can induce resistance to dengue infection in a Wolbachia-density dependent and a tissue-specific manner [18]. To test whether the above viral inhibition is caused by an increased Wolbachia density in the somatic tissues of MTB mosquitoes, we compared the Wolbachia density between MTB and APM mosquitoes using qPCR. We found that the Wolbachia density is significantly higher in the salivary glands (5,738-fold), fat bodies (68-fold), and midguts (269-fold) of MTB mosquitoes as compared to APM mosquitoes, while no differences were observed in the ovaries (1-fold) of APM and MTB mosquitoes (Fig. 4). These results suggest that the high density of Wolbachia in somatic tissues of MTB mosquitoes may contribute directly to their low susceptibility for DENV.

Bottom Line: In a previous study, the native wPolA in Ae. polynesiensis was replaced with wAlbB from Ae. albopictus, and resulted in the generation of the transinfected "MTB" strain with low susceptibility for filarial worms.The results show that wAlbB can induce a strong resistance to DENV-2 in the MTB mosquito.Evidence also supports that this resistance is related to a dramatic increase in Wolbachia density in the MTB's somatic tissues, including the midgut and salivary gland.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan, United States of America.

ABSTRACT
Wolbachia is a maternally transmitted endosymbiotic bacterium that is estimated to infect up to 65% of insect species. The ability of Wolbachia to both induce pathogen interference and spread into mosquito vector populations makes it possible to develop Wolbachia as a biological control agent for vector-borne disease control. Although Wolbachia induces resistance to dengue virus (DENV), filarial worms, and Plasmodium in mosquitoes, species like Aedes polynesiensis and Aedes albopictus, which carry native Wolbachia infections, are able to transmit dengue and filariasis. In a previous study, the native wPolA in Ae. polynesiensis was replaced with wAlbB from Ae. albopictus, and resulted in the generation of the transinfected "MTB" strain with low susceptibility for filarial worms. In this study, we compare the dynamics of DENV serotype 2 (DENV-2) within the wild type "APM" strain and the MTB strain of Ae. polynesiensis by measuring viral infection in the mosquito whole body, midgut, head, and saliva at different time points post infection. The results show that wAlbB can induce a strong resistance to DENV-2 in the MTB mosquito. Evidence also supports that this resistance is related to a dramatic increase in Wolbachia density in the MTB's somatic tissues, including the midgut and salivary gland. Our results suggests that replacement of a native Wolbachia with a novel infection could serve as a strategy for developing a Wolbachia-based approach to target naturally infected insects for vector-borne disease control.

Show MeSH
Related in: MedlinePlus