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Preferential killing of human lung cancer cell lines with mitochondrial dysfunction by nonthermal dielectric barrier discharge plasma.

Panngom K, Baik KY, Nam MK, Han JH, Rhim H, Choi EH - Cell Death Dis (2013)

Bottom Line: It is found that the cell number of lung cancer cells has been reduced more than that of the lung normal cells.The mitochondrial vulnerability to reactive species in H460 may induce distinctively selective responses.Differential mitochondrial membrane potential decrease, mitochondrial enzymatic dysfunction, and mitochondrial morphological alteration are exhibited in two cell lines.

View Article: PubMed Central - PubMed

Affiliation: Department of Plasma Bioscience and Display, Kwangwoon University, Seoul, Republic of Korea.

ABSTRACT
The distinctive cellular and mitochondrial dysfunctions of two human lung cancer cell lines (H460 and HCC1588) from two human lung normal cell lines (MRC5 and L132) have been studied by dielectric barrier discharge (DBD) plasma treatment. This cytotoxicity is exposure time-dependent, which is strongly mediated by the large amount of H2O2 and NOx in culture media generated by DBD nonthermal plasma. It is found that the cell number of lung cancer cells has been reduced more than that of the lung normal cells. The mitochondrial vulnerability to reactive species in H460 may induce distinctively selective responses. Differential mitochondrial membrane potential decrease, mitochondrial enzymatic dysfunction, and mitochondrial morphological alteration are exhibited in two cell lines. These results suggest the nonthermal plasma treatment as an efficacious modality in lung cancer therapy.

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DBD plasma effects on mitochondrial metabolic activity of H460 and MRC5 after 24 h from treatment. (a) The relative MTS value of H460 and MRC5, and (b) the relative ATP value of H460 and MRC5 in galactose media. H460 showed much decreased mitochondrial enzymatic activity with plasma exposure time in both assays. Students' t-test was performed to control (* denotes P<0.05 and ** denotes P<0.01)
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fig5: DBD plasma effects on mitochondrial metabolic activity of H460 and MRC5 after 24 h from treatment. (a) The relative MTS value of H460 and MRC5, and (b) the relative ATP value of H460 and MRC5 in galactose media. H460 showed much decreased mitochondrial enzymatic activity with plasma exposure time in both assays. Students' t-test was performed to control (* denotes P<0.05 and ** denotes P<0.01)

Mentions: In order to examine the distinctive nonthermal plasma effects on mitochondria that has important roles in apoptosis, we measured MTS reduction and ATP generation in H460 and MRC5. MTS reduction is mainly attributed to mitochondrial enzymes and electron carriers,20 which indicates the mitochondrial respiratory activity. Their final product ATP was quantified by luciferase in galactose-containing cell culture media.21 Galactose was used instead of glucose to exclude the ATP generation through glycolysis in cytoplasm in cancer cells.22, 23 Interestingly, plasma induced significant changes in MTS reduction and ATP production with almost exactly same tendency (Figures 5a and b). Both relative values reduced more with plasma exposure time increase at day 1. The effect of 1-min-plasma exposure was almost negligible; however, longer plasma exposure (3 and 5 min exposure) induced significant reduction in mitochondrial enzyme activity as well as mitochondrial malfunction, especially in H460. These reductions in mitochondrial respiration show similar tendency to the increase of Annexin V-positive apoptosis.


Preferential killing of human lung cancer cell lines with mitochondrial dysfunction by nonthermal dielectric barrier discharge plasma.

Panngom K, Baik KY, Nam MK, Han JH, Rhim H, Choi EH - Cell Death Dis (2013)

DBD plasma effects on mitochondrial metabolic activity of H460 and MRC5 after 24 h from treatment. (a) The relative MTS value of H460 and MRC5, and (b) the relative ATP value of H460 and MRC5 in galactose media. H460 showed much decreased mitochondrial enzymatic activity with plasma exposure time in both assays. Students' t-test was performed to control (* denotes P<0.05 and ** denotes P<0.01)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3674375&req=5

fig5: DBD plasma effects on mitochondrial metabolic activity of H460 and MRC5 after 24 h from treatment. (a) The relative MTS value of H460 and MRC5, and (b) the relative ATP value of H460 and MRC5 in galactose media. H460 showed much decreased mitochondrial enzymatic activity with plasma exposure time in both assays. Students' t-test was performed to control (* denotes P<0.05 and ** denotes P<0.01)
Mentions: In order to examine the distinctive nonthermal plasma effects on mitochondria that has important roles in apoptosis, we measured MTS reduction and ATP generation in H460 and MRC5. MTS reduction is mainly attributed to mitochondrial enzymes and electron carriers,20 which indicates the mitochondrial respiratory activity. Their final product ATP was quantified by luciferase in galactose-containing cell culture media.21 Galactose was used instead of glucose to exclude the ATP generation through glycolysis in cytoplasm in cancer cells.22, 23 Interestingly, plasma induced significant changes in MTS reduction and ATP production with almost exactly same tendency (Figures 5a and b). Both relative values reduced more with plasma exposure time increase at day 1. The effect of 1-min-plasma exposure was almost negligible; however, longer plasma exposure (3 and 5 min exposure) induced significant reduction in mitochondrial enzyme activity as well as mitochondrial malfunction, especially in H460. These reductions in mitochondrial respiration show similar tendency to the increase of Annexin V-positive apoptosis.

Bottom Line: It is found that the cell number of lung cancer cells has been reduced more than that of the lung normal cells.The mitochondrial vulnerability to reactive species in H460 may induce distinctively selective responses.Differential mitochondrial membrane potential decrease, mitochondrial enzymatic dysfunction, and mitochondrial morphological alteration are exhibited in two cell lines.

View Article: PubMed Central - PubMed

Affiliation: Department of Plasma Bioscience and Display, Kwangwoon University, Seoul, Republic of Korea.

ABSTRACT
The distinctive cellular and mitochondrial dysfunctions of two human lung cancer cell lines (H460 and HCC1588) from two human lung normal cell lines (MRC5 and L132) have been studied by dielectric barrier discharge (DBD) plasma treatment. This cytotoxicity is exposure time-dependent, which is strongly mediated by the large amount of H2O2 and NOx in culture media generated by DBD nonthermal plasma. It is found that the cell number of lung cancer cells has been reduced more than that of the lung normal cells. The mitochondrial vulnerability to reactive species in H460 may induce distinctively selective responses. Differential mitochondrial membrane potential decrease, mitochondrial enzymatic dysfunction, and mitochondrial morphological alteration are exhibited in two cell lines. These results suggest the nonthermal plasma treatment as an efficacious modality in lung cancer therapy.

Show MeSH
Related in: MedlinePlus