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Quantitative phase imaging techniques for the study of cell pathophysiology: from principles to applications.

Lee K, Kim K, Jung J, Heo J, Cho S, Lee S, Chang G, Jo Y, Park H, Park Y - Sensors (Basel) (2013)

Bottom Line: A cellular-level study of the pathophysiology is crucial for understanding the mechanisms behind human diseases.Recent advances in quantitative phase imaging (QPI) techniques show promises for the cellular-level understanding of the pathophysiology of diseases.To provide important insight on how the QPI techniques potentially improve the study of cell pathophysiology, here we present the principles of QPI and highlight some of the recent applications of QPI ranging from cell homeostasis to infectious diseases and cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Physics, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea. kyeo@kaist.ac.kr

ABSTRACT
A cellular-level study of the pathophysiology is crucial for understanding the mechanisms behind human diseases. Recent advances in quantitative phase imaging (QPI) techniques show promises for the cellular-level understanding of the pathophysiology of diseases. To provide important insight on how the QPI techniques potentially improve the study of cell pathophysiology, here we present the principles of QPI and highlight some of the recent applications of QPI ranging from cell homeostasis to infectious diseases and cancer.

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The study of cell pathology using QPI (A) Cross-sectional images of 3-D RI tomograms of Pf-RBCs measured by TPM. Black arrows: parasitophorous vacuole; gray arrows: hemozoin; (B) Topograms of RBCs from a SCD patient, measured by DPM. II-IV: classification based on morphology; (C) morphology of rat basophilic leukemia RBL-2H3 cells infected with V. vulnificus strains; (D) Phase image of high-definition-CTC measured by non-interferometric quantitative phase microscopy. (A–D) are modified from refs. [78,112,127], and [132], respectively, with permissions.
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f4-sensors-13-04170: The study of cell pathology using QPI (A) Cross-sectional images of 3-D RI tomograms of Pf-RBCs measured by TPM. Black arrows: parasitophorous vacuole; gray arrows: hemozoin; (B) Topograms of RBCs from a SCD patient, measured by DPM. II-IV: classification based on morphology; (C) morphology of rat basophilic leukemia RBL-2H3 cells infected with V. vulnificus strains; (D) Phase image of high-definition-CTC measured by non-interferometric quantitative phase microscopy. (A–D) are modified from refs. [78,112,127], and [132], respectively, with permissions.

Mentions: While healthy RBCs show uniform RI distribution, Pf-RBCs are optically inhomogeneous; they have vacuoles of parasites with low RI and the hemozoin crystals, insoluble polymerized forms of heme with high RI.These RI information can be used to determine the Hb concentration (Figure 4(A)) [78]. Using 3D QPI, the dynamics of the egress process of malaria infection have been investigated [86], which demonstrated that the inhibitors E64d and EGTA-AM prevent the merozoites from escaping host Pf-RBCs. Utilizing the DPM in combination with genetic knock-out technique, it has been shown thatPf155/Ring-Infected Erythrocyte Surface Antigen (RESA) are responsible for the decreased dynamic microcirculatory behavior of ring-stage Pf-RBCs [126]. In addition, analyzing the light scattering of the individual Pf-RBCs through the DPM and FTLS techniques, the specific disease state of Pf-RBC were identified and the alterations in mechanical properties of the cell membranes were determined [68].Other than malaria infection, QPI has been used for studying the morphology and volumes of rat basophilic leukemia RBL-2H3 cells infected with V. vulnificus strains (Figure 4(C)) [127], and the cellular damage induced by Shiga toxin in human brain microvascular endothelial cells, which results in haemolytic uraemic syndrome caused by enterohaemorrhagic E. coli [128]. QPI can also be used for the study of cytotoxicity assessment [129].


Quantitative phase imaging techniques for the study of cell pathophysiology: from principles to applications.

Lee K, Kim K, Jung J, Heo J, Cho S, Lee S, Chang G, Jo Y, Park H, Park Y - Sensors (Basel) (2013)

The study of cell pathology using QPI (A) Cross-sectional images of 3-D RI tomograms of Pf-RBCs measured by TPM. Black arrows: parasitophorous vacuole; gray arrows: hemozoin; (B) Topograms of RBCs from a SCD patient, measured by DPM. II-IV: classification based on morphology; (C) morphology of rat basophilic leukemia RBL-2H3 cells infected with V. vulnificus strains; (D) Phase image of high-definition-CTC measured by non-interferometric quantitative phase microscopy. (A–D) are modified from refs. [78,112,127], and [132], respectively, with permissions.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3673078&req=5

f4-sensors-13-04170: The study of cell pathology using QPI (A) Cross-sectional images of 3-D RI tomograms of Pf-RBCs measured by TPM. Black arrows: parasitophorous vacuole; gray arrows: hemozoin; (B) Topograms of RBCs from a SCD patient, measured by DPM. II-IV: classification based on morphology; (C) morphology of rat basophilic leukemia RBL-2H3 cells infected with V. vulnificus strains; (D) Phase image of high-definition-CTC measured by non-interferometric quantitative phase microscopy. (A–D) are modified from refs. [78,112,127], and [132], respectively, with permissions.
Mentions: While healthy RBCs show uniform RI distribution, Pf-RBCs are optically inhomogeneous; they have vacuoles of parasites with low RI and the hemozoin crystals, insoluble polymerized forms of heme with high RI.These RI information can be used to determine the Hb concentration (Figure 4(A)) [78]. Using 3D QPI, the dynamics of the egress process of malaria infection have been investigated [86], which demonstrated that the inhibitors E64d and EGTA-AM prevent the merozoites from escaping host Pf-RBCs. Utilizing the DPM in combination with genetic knock-out technique, it has been shown thatPf155/Ring-Infected Erythrocyte Surface Antigen (RESA) are responsible for the decreased dynamic microcirculatory behavior of ring-stage Pf-RBCs [126]. In addition, analyzing the light scattering of the individual Pf-RBCs through the DPM and FTLS techniques, the specific disease state of Pf-RBC were identified and the alterations in mechanical properties of the cell membranes were determined [68].Other than malaria infection, QPI has been used for studying the morphology and volumes of rat basophilic leukemia RBL-2H3 cells infected with V. vulnificus strains (Figure 4(C)) [127], and the cellular damage induced by Shiga toxin in human brain microvascular endothelial cells, which results in haemolytic uraemic syndrome caused by enterohaemorrhagic E. coli [128]. QPI can also be used for the study of cytotoxicity assessment [129].

Bottom Line: A cellular-level study of the pathophysiology is crucial for understanding the mechanisms behind human diseases.Recent advances in quantitative phase imaging (QPI) techniques show promises for the cellular-level understanding of the pathophysiology of diseases.To provide important insight on how the QPI techniques potentially improve the study of cell pathophysiology, here we present the principles of QPI and highlight some of the recent applications of QPI ranging from cell homeostasis to infectious diseases and cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Physics, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea. kyeo@kaist.ac.kr

ABSTRACT
A cellular-level study of the pathophysiology is crucial for understanding the mechanisms behind human diseases. Recent advances in quantitative phase imaging (QPI) techniques show promises for the cellular-level understanding of the pathophysiology of diseases. To provide important insight on how the QPI techniques potentially improve the study of cell pathophysiology, here we present the principles of QPI and highlight some of the recent applications of QPI ranging from cell homeostasis to infectious diseases and cancer.

Show MeSH
Related in: MedlinePlus