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Quantitative phase imaging techniques for the study of cell pathophysiology: from principles to applications.

Lee K, Kim K, Jung J, Heo J, Cho S, Lee S, Chang G, Jo Y, Park H, Park Y - Sensors (Basel) (2013)

Bottom Line: A cellular-level study of the pathophysiology is crucial for understanding the mechanisms behind human diseases.Recent advances in quantitative phase imaging (QPI) techniques show promises for the cellular-level understanding of the pathophysiology of diseases.To provide important insight on how the QPI techniques potentially improve the study of cell pathophysiology, here we present the principles of QPI and highlight some of the recent applications of QPI ranging from cell homeostasis to infectious diseases and cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Physics, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea. kyeo@kaist.ac.kr

ABSTRACT
A cellular-level study of the pathophysiology is crucial for understanding the mechanisms behind human diseases. Recent advances in quantitative phase imaging (QPI) techniques show promises for the cellular-level understanding of the pathophysiology of diseases. To provide important insight on how the QPI techniques potentially improve the study of cell pathophysiology, here we present the principles of QPI and highlight some of the recent applications of QPI ranging from cell homeostasis to infectious diseases and cancer.

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Related in: MedlinePlus

Principles of QPI (A) Conventional bright-field imaging measures amplitude information only; (B) QPI employs the principle of interferometry or holography, and measures both amplitude and phase information.
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f1-sensors-13-04170: Principles of QPI (A) Conventional bright-field imaging measures amplitude information only; (B) QPI employs the principle of interferometry or holography, and measures both amplitude and phase information.

Mentions: QPI techniques employ the principle of interferometry to measure the optical field, consisting of amplitude and phase information, whereas conventional bright-field imaging only measures the amplitude (Figure 1). Since most biological samples, including biomolecules, cells, and tissues, are optically transparent in visible light, information of amplitude does not provide good contrast for imaging. However, even these transparent samples provide significant optical phase delay, which serves as imaging contrast for QPI. Full details of the QPI techniques can be found elsewhere [1].


Quantitative phase imaging techniques for the study of cell pathophysiology: from principles to applications.

Lee K, Kim K, Jung J, Heo J, Cho S, Lee S, Chang G, Jo Y, Park H, Park Y - Sensors (Basel) (2013)

Principles of QPI (A) Conventional bright-field imaging measures amplitude information only; (B) QPI employs the principle of interferometry or holography, and measures both amplitude and phase information.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3673078&req=5

f1-sensors-13-04170: Principles of QPI (A) Conventional bright-field imaging measures amplitude information only; (B) QPI employs the principle of interferometry or holography, and measures both amplitude and phase information.
Mentions: QPI techniques employ the principle of interferometry to measure the optical field, consisting of amplitude and phase information, whereas conventional bright-field imaging only measures the amplitude (Figure 1). Since most biological samples, including biomolecules, cells, and tissues, are optically transparent in visible light, information of amplitude does not provide good contrast for imaging. However, even these transparent samples provide significant optical phase delay, which serves as imaging contrast for QPI. Full details of the QPI techniques can be found elsewhere [1].

Bottom Line: A cellular-level study of the pathophysiology is crucial for understanding the mechanisms behind human diseases.Recent advances in quantitative phase imaging (QPI) techniques show promises for the cellular-level understanding of the pathophysiology of diseases.To provide important insight on how the QPI techniques potentially improve the study of cell pathophysiology, here we present the principles of QPI and highlight some of the recent applications of QPI ranging from cell homeostasis to infectious diseases and cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Physics, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea. kyeo@kaist.ac.kr

ABSTRACT
A cellular-level study of the pathophysiology is crucial for understanding the mechanisms behind human diseases. Recent advances in quantitative phase imaging (QPI) techniques show promises for the cellular-level understanding of the pathophysiology of diseases. To provide important insight on how the QPI techniques potentially improve the study of cell pathophysiology, here we present the principles of QPI and highlight some of the recent applications of QPI ranging from cell homeostasis to infectious diseases and cancer.

Show MeSH
Related in: MedlinePlus