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Optimalization of Poly(neutral red) Coated-wire Electrode for Determination of Citrate in Soft Drinks

View Article: PubMed Central - PubMed

ABSTRACT

This report presents an optimization of potentiometric measurements with citrate-selective electropolymerized poly(neutral red) electrodes. The optimal background electrolyte for these measurements is a TRIS buffer with nitrate at pH 8.5. The electrodes described here exhibit stable and reproducible near-Nernstian response to citrates with a low detection limit of 6 × 10-6 M. Electrodes polymerized from sulfuric acid and acetonitrile are compared in detail. Simple and sensitive method for quantification of citrate in real-life samples by potentiometry with poly(neutral red) electrodes are presented. Data from potentiometric measurements of citrate are compared with capillary electrophoresis.

No MeSH data available.


Related in: MedlinePlus

Citrate electrophoregrams of soft drinks. The citrate peak is marked by a rectangle; 1 – Joe's cola, 2 – Mountain Dew, 3 – Kofola, 4 – Zón, 5 – Poděbradka, 6 – Gatorade, 7 – Hello.
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f1-sensors-08-00594: Citrate electrophoregrams of soft drinks. The citrate peak is marked by a rectangle; 1 – Joe's cola, 2 – Mountain Dew, 3 – Kofola, 4 – Zón, 5 – Poděbradka, 6 – Gatorade, 7 – Hello.

Mentions: The samples were injected into the capillary hydrodynamically (1.5 kPa/30 s) and the analyses were run at reverse polarity, -10 kV, at 20 °C for 8 min. The instrument was first calibrated using standards of citrate in water at concentrations from 0.01 mM to 0.2 mM. The peak citrate concentration was registered at around the 6th minute. The 7 soft drinks were then analyzed. The samples were diluted 50- or 100-fold before analysis. Figure 1 shows electrophoregrams of the samples. 6 analyses were carried out of each standard and sample. The citrate contents were calculated from the peak areas.


Optimalization of Poly(neutral red) Coated-wire Electrode for Determination of Citrate in Soft Drinks
Citrate electrophoregrams of soft drinks. The citrate peak is marked by a rectangle; 1 – Joe's cola, 2 – Mountain Dew, 3 – Kofola, 4 – Zón, 5 – Poděbradka, 6 – Gatorade, 7 – Hello.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3672997&req=5

f1-sensors-08-00594: Citrate electrophoregrams of soft drinks. The citrate peak is marked by a rectangle; 1 – Joe's cola, 2 – Mountain Dew, 3 – Kofola, 4 – Zón, 5 – Poděbradka, 6 – Gatorade, 7 – Hello.
Mentions: The samples were injected into the capillary hydrodynamically (1.5 kPa/30 s) and the analyses were run at reverse polarity, -10 kV, at 20 °C for 8 min. The instrument was first calibrated using standards of citrate in water at concentrations from 0.01 mM to 0.2 mM. The peak citrate concentration was registered at around the 6th minute. The 7 soft drinks were then analyzed. The samples were diluted 50- or 100-fold before analysis. Figure 1 shows electrophoregrams of the samples. 6 analyses were carried out of each standard and sample. The citrate contents were calculated from the peak areas.

View Article: PubMed Central - PubMed

ABSTRACT

This report presents an optimization of potentiometric measurements with citrate-selective electropolymerized poly(neutral red) electrodes. The optimal background electrolyte for these measurements is a TRIS buffer with nitrate at pH 8.5. The electrodes described here exhibit stable and reproducible near-Nernstian response to citrates with a low detection limit of 6 × 10-6 M. Electrodes polymerized from sulfuric acid and acetonitrile are compared in detail. Simple and sensitive method for quantification of citrate in real-life samples by potentiometry with poly(neutral red) electrodes are presented. Data from potentiometric measurements of citrate are compared with capillary electrophoresis.

No MeSH data available.


Related in: MedlinePlus